| http://purl.uniprot.org/citations/7545961 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7545961 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7545961 | http://www.w3.org/2000/01/rdf-schema#comment | "Conjugative transfer of the Enterococcus faecalis tetracycline resistance plasmid pCF10 is stimulated by a peptide pheromone, cCF10. Once a recipient strain acquires pCF10 and thus becomes a pheromone-responsive donor, cCF10 activity is no longer detected in culture filtrates. Here we show that pCF10 encodes a peptide inhibitor, iCF10, secreted by donor cells; this inhibitor antagonizes the cCF10 activity in culture filtrates. In order to detect and quantitate iCF10, we developed a reverse-phase high-performance liquid chromatography assay in which the inhibitor peptide elutes separately from the pheromone; this type of assay enabled us to determine that lack of pheromone activity in donor culture filtrates was due to secretion of a mixture of iCF10 and cCF10, rather than abolition of cCF10 secretion. The gene encoding iCF10, prgQ, is located on the EcoRI-C fragment of pCF10. The open reading frame comprising the prgQ gene encodes a 23-amino-acid precursor that resembles a signal peptide. This precursor is cleaved to the mature heptapeptide iCF10 during the secretion process."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.176.23.7405-7408.1994"xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Nakayama J."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Nakayama J."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Suzuki A."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Suzuki A."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Dunny G.M."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Dunny G.M."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Isogai A."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Isogai A."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Ruhfel R.E."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/author | "Ruhfel R.E."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/name | "J Bacteriol"xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/pages | "7405-7408"xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/pages | "7405-7408"xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/title | "The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/title | "The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10."xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/volume | "176"xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://purl.uniprot.org/core/volume | "176"xsd:string |
| http://purl.uniprot.org/citations/7545961 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7545961 |