| http://purl.uniprot.org/citations/7592673 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7592673 | http://www.w3.org/2000/01/rdf-schema#comment | "Interleukin-5 (IL-5) plays a central role in the growth and differentiation of eosinophils and contributes to several disease states including asthma. Accumulating evidence suggests a role for cAMP as an immunomodulator; agents that increase intracellular cAMP levels have been shown to inhibit production of cytokines predominantly produced by T helper (Th) 1 cells such as IL-2 and interferon gamma (IFN-gamma). In contrast, the production of IL-5, predominantly produced by Th2 cells, is actually enhanced by these agents. In this report, we have performed transient transfection experiments with IL-5 promoter-reporter gene constructs, DNase I footprinting assays, and electrophoretic mobility shift assays to investigate the key regulatory regions necessary for activation of the IL-5 promoter by dibutyryl cAMP and phorbol esters in the mouse thymoma line EL-4. Taken together, our data demonstrate the critical importance of two sequences within the IL-5 5'-flanking region for activation by these agents in EL-4 cells: one, a highly conserved 15-base pair element present in genes expressed by Th2 cells, called the conserved lymphokine element 0 (CLE0; located between -53 and -39 in the IL-5 promoter), and the other, two overlapping binding sites for the transcription factor GATA-3 (but not GATA-4) between -70 and -59. Taken together, our data suggest that activation via the unique sequence combination GATA/CLE0 results in selective expression of the IL-5 gene in response to elevated levels of intracellular cAMP."xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.41.24548"xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/author | "Ray P."xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/author | "Ray A."xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/author | "Zhang D.H."xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/author | "Siegel M.D."xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/pages | "24548-24555"xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/title | "Activation of the interleukin-5 promoter by cAMP in murine EL-4 cells requires the GATA-3 and CLE0 elements."xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7592673 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7592673 |
| http://purl.uniprot.org/citations/7592673 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7592673 |
| http://purl.uniprot.org/uniprot/P23771#attribution-3340B23F5138DB4C2EF87A86C70541AB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/7592673 |