| http://purl.uniprot.org/citations/7737162 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7737162 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7737162 | http://www.w3.org/2000/01/rdf-schema#comment | "PC-1 is an ecto-enzyme possessing alkaline phosphodiesterase I (EC 3.1.4.1) and nucleotide pyrophosphatase (EC 3.6.1.9) activities. It has also been proposed to be an ecto-protein kinase capable of phosphorylating itself as well as exogenous proteins. We have investigated the phosphorylation capability of PC-1 and have developed a novel method for its detection and characterization based on autophosphorylation, which allows detection without the use of antibodies. When cells expressing membrane PC-1 were held on ice with [gamma-32P]ATP, SDS/PAGE of whole cell lysates showed a single band which was PC-1; this band was absent in cells not expressing PC-1. Immunoprecipitates of soluble PC-1 isolated from culture supernatants of cells expressing PC-1 were also capable of autophosphorylation, and the size of the labeled protein was the same as previously reported for soluble PC-1. PC-1 was also labeled with [alpha-32P]ATP and [35S]dATP[alpha S]. We found no evidence that PC-1 was capable of phosphorylating proteins other than itself, and conclude that it is not a true kinase, and that the observed labeling with [gamma-32P]ATP, [alpha-32P]ATP and [35S]dATP[alpha S] reflect transient covalent adducts that are part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity rather than intrinsic kinase activity. Mutation of the active-site threonine to tyrosine, serine or alanine reduced the 5'-nucleotide phosphodiesterase activity of PC-1 and its ability to autophosphorylate to undetectable levels. Together, these data suggest that both activities depend on the same site."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1995.tb20308.x"xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1995.tb20308.x"xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Sali A."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Sali A."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Belli S.I."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Belli S.I."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Goding J.W."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Goding J.W."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Mercuri F.A."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/author | "Mercuri F.A."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/pages | "669-676"xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/pages | "669-676"xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/title | "Autophosphorylation of PC-1 (alkaline phosphodiesterase I/nucleotide pyrophosphatase) and analysis of the active site."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/title | "Autophosphorylation of PC-1 (alkaline phosphodiesterase I/nucleotide pyrophosphatase) and analysis of the active site."xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/volume | "228"xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://purl.uniprot.org/core/volume | "228"xsd:string |
| http://purl.uniprot.org/citations/7737162 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7737162 |
| http://purl.uniprot.org/citations/7737162 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7737162 |