| http://purl.uniprot.org/citations/7845680 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7845680 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7845680 | http://www.w3.org/2000/01/rdf-schema#comment | "The Epstein-Barr virus (EBV) encoded latent membrane protein, LMP1, is oncogenic in rodent fibroblasts and is an essential effector protein in EBV-induced growth-transformation of human B lymphocytes. Previous structure-function studies with LMP1 have relied largely on rodent fibroblast transformation as a functional readout, with apparently conflicting results. We have now analysed several LMP1 mutants in various human cell types, including B cells, T cells and epithelial cells, using two independent functional assays; (i) activation of NF-kappa B, and (ii) induction of two cell surface activation markers, CD54 and CD40. The results suggest that the cytosolic N-terminus is not essential for LMP1 function in any cell type studied. The third and fourth transmembrane helices and the intracytosolic loops are dispensable for activation of NF-kappa B, but they do influence the induction of CD54 and CD40. The major effector domain appears to be the cytosolic C-terminus in which were identified two 'C-terminal activating regions', CTAR-1 (residues 194-232) and CTAR-2 (residues 351-386). Whilst the exact results depended upon the host cell line, CTAR-2 was generally more important for activation of NF-kappa B, and both CTAR-1 and CTAR-2 were required for optimal induction of CD54 and CD40. Analysis of NF-kappa B activation by LMP1 in Rat-1 fibroblasts indicated that many mutations that were functional in human cells were poorly tolerated in the rodent cells; a result that is in broad agreement with published Rat-1 transformation data."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Rowe M."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Rowe M."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Croom-Carter D."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Croom-Carter D."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Henderson S.A."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Henderson S.A."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Huen D.S."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/author | "Huen D.S."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/pages | "549-560"xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/pages | "549-560"xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/title | "The Epstein-Barr virus latent membrane protein-1 (LMP1) mediates activation of NF-kappa B and cell surface phenotype via two effector regions in its carboxy-terminal cytoplasmic domain."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/title | "The Epstein-Barr virus latent membrane protein-1 (LMP1) mediates activation of NF-kappa B and cell surface phenotype via two effector regions in its carboxy-terminal cytoplasmic domain."xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/7845680 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7845680 |
| http://purl.uniprot.org/citations/7845680 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7845680 |
| http://purl.uniprot.org/citations/7845680 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7845680 |
| http://purl.uniprot.org/citations/7845680 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7845680 |