| http://purl.uniprot.org/citations/7852353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7852353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7852353 | http://www.w3.org/2000/01/rdf-schema#comment | "The thrombospondins are a family of extracellular calcium binding proteins that are involved in cell proliferation, adhesion, and migration. We have sequenced full-length human thrombospondin-4 and characterized the recombinant protein. In contrast to Xenopus laevis thrombospondin-4, the human protein contains an RGD cell binding sequence in the third type 3 repeat. Transfection of mouse NIH3T3 fibroblasts or C2C12 myoblasts with a full-length human thrombospondin-4 cDNA results in the expression of a polypeptide with a reduced molecular weight of 140,000. In the absence of reducing agent, the expressed protein has an apparent molecular weight of 550,000. Recombinant thrombospondin-4 has been purified from the culture supernatant by heparin-Sepharose and anti-thrombospondin-4 antibody-Affi-gel affinity chromatography. Electron microscopy indicates that thrombospondin-4 is composed of five subunits with globular domains at each end. The observation of a calcium-dependent change in the electron microscopic appearance of thrombospondin-4 is consistent with limited tryptic digestion data that indicate that thrombospondin-4 is resistant to digestion in the presence of calcium. These data indicate that thrombospondin-4 is a pentameric protein that binds to heparin and calcium."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.6.2809"xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.6.2809"xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "Lawler J."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "Lawler J."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "Duquette M."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "Duquette M."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "Derick L."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "Derick L."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "McHenry K."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/author | "McHenry K."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/pages | "2809-2814"xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/pages | "2809-2814"xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/title | "Characterization of human thrombospondin-4."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/title | "Characterization of human thrombospondin-4."xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://purl.uniprot.org/core/volume | "270"xsd:string |
| http://purl.uniprot.org/citations/7852353 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7852353 |
| http://purl.uniprot.org/citations/7852353 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7852353 |