| http://purl.uniprot.org/citations/7890771 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7890771 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7890771 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously described a C-terminally truncated variant of the chemokine neutrophil-activating peptide 2 (NAP-2) that exhibited higher neutrophil-stimulating capacity than the full-size polypeptide. To investigate the impact of the NAP-2 C terminus on biological activity and receptor binding, we have now purified the novel molecule to homogeneity. Furthermore, we have cloned, expressed in Escherichia coli, and purified full-size recombinant NAP-2 (rNAP-2-(1-70)) and a series of C-terminally deleted variants (rNAP-2-(1-69) to rNAP-2-(1-64)). Biochemical and immunochemical analyses revealed that the natural NAP-2 variant was structurally identical to the rNAP-2-(1-66) isoform. As compared with their respective native and recombinant full-size counterparts, both molecules exhibited approximately 3-4-fold enhanced potency in the induction of neutrophil degranulation as well as 3-fold enhanced binding affinity for specific receptors on these cells. All other variants were considerably less active. The natural occurrence of a NAP-2 variant truncated by exactly four residues at the C terminus suggests that limited and defined proteolysis at this site plays a role in the regulation of the biological function of the chemokine."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.11.6338"xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.270.11.6338"xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Petersen F."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Petersen F."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Flad H.D."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Flad H.D."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Gerdes J."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Gerdes J."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Brandt E."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Brandt E."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Ehlert J.E."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Ehlert J.E."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Kubbutat M.H."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/author | "Kubbutat M.H."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/date | "1995"xsd:gYear |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/pages | "6338-6344"xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/pages | "6338-6344"xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/title | "Limited and defined truncation at the C-terminus enhances receptor binding and degranulation activity of the neutrophil-activating peptide 2 (NAP-2). Comparison of native and recombinant NAP-2 variants."xsd:string |
| http://purl.uniprot.org/citations/7890771 | http://purl.uniprot.org/core/title | "Limited and defined truncation at the C-terminus enhances receptor binding and degranulation activity of the neutrophil-activating peptide 2 (NAP-2). Comparison of native and recombinant NAP-2 variants."xsd:string |