| http://purl.uniprot.org/citations/7983070 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7983070 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/7983070 | http://www.w3.org/2000/01/rdf-schema#comment | "The subcellular distribution and targeting of the non-lysosomal aspartic proteinase cathepsin E have been studied using mouse L cells and monkey Cos 1 cells that were transfected with cDNA encoding cathepsin E. The cathepsin E was retained in L cells for at least 20 h without significant degradation and its single N-linked oligosaccharide remained sensitive to endo-beta-N-acetyl-glucosaminidase H. When cathepsin E was overexpressed by transient transfection in Cos 1 cells, it was very slowly secreted into the media. The intracellular form of the enzyme contained a high mannose oligosaccharide which was processed to a complex type species upon secretion. In double label immunofluorescence studies, cathepsin E co-localized with cathepsin D-myc-KDEL, an endoplasmic reticulum (ER) marker. Subcellular fractionation on a Percoll density gradient showed that the cathepsin E co-migrated with membranous vesicles that were distinct from dense lysosomes. Only a trace amount of the enzyme was recovered in the soluble fraction. These findings indicate that in L cells and Cos 1 cells, the intracellular location of cathepsin E is the endoplasmic reticulum. To identify the protein sequences required for ER retention, we made chimeric proteins between cathepsin E and pepsinogen, an aspartic proteinase that is rapidly secreted by Cos 1 cells. We found that amino acids 1-48 of cathepsin E are important for its retention in the ER. Within this region, Cys7, which is involved in covalent dimer formation, plays a significant role in the retention."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)47417-0"xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)47417-0"xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/author | "Kornfeld S."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/author | "Kornfeld S."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/author | "Finley E.M."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/author | "Finley E.M."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/pages | "31259-31266"xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/pages | "31259-31266"xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/title | "Subcellular localization and targeting of cathepsin E."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/title | "Subcellular localization and targeting of cathepsin E."xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://purl.uniprot.org/core/volume | "269"xsd:string |
| http://purl.uniprot.org/citations/7983070 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7983070 |
| http://purl.uniprot.org/citations/7983070 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/7983070 |
| http://purl.uniprot.org/citations/7983070 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7983070 |
| http://purl.uniprot.org/citations/7983070 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/7983070 |
| http://purl.uniprot.org/uniprot/P14091 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7983070 |
| http://purl.uniprot.org/uniprot/P70269 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/7983070 |