| http://purl.uniprot.org/citations/8034987 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8034987 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8034987 | http://www.w3.org/2000/01/rdf-schema#comment | "The clonality of immunoglobulins (Igs) in cryoprecipitates (n = 41) was studied by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Our series included 24 cryoglobulins characterized by immunofixation electrophoresis (IF), 12 'trace amount' cryoglobulins, defined by a protein content in the precipitate of less than 0.05 mg/ml of serum, and five cryoglobulins of undetermined protein composition by IF. 2-D PAGE analysis showed polyclonal IgG associated either with monoclonal Igs (type II cryoglobulins; n = 14) or with polyclonal IgM (type III cryoglobulins; n = 14). In ten cryoprecipitates (two 'trace amount' cryoglobulins as well as seven of 19 type II and as one of five type III cryoglobulins by IF) polyclonal IgG were associated with a mixture of polyclonal and monoclonal IgM. These cryoglobulins were tentatively named type II-III cryoglobulins. A monoclonal IgM was observed in one cryoprecipitate (type I cryoglobulins). Two cryoglobulins presented unexpected 2-D patterns, characterized by the presence of oligoclonal IgM, with trace amounts of Igs of different isotypes (tentatively named type II-III(variant) cryoglobulins). A peptide of 44 kDa with a pI of 5.45 was observed in all cryoglobulins containing IgM (n = 40). This peptide was also present in purified monoclonal or polyclonal IgM fractions. N-terminal microsequencing (12 amino acid residues) revealed that this IgM-associated peptide was an unknown protein. Our results highlight the role of 2-D PAGE as an aid in the analysis of cryoglobulins."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.org/dc/terms/identifier | "doi:10.1016/0022-1759(94)90284-4"xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.org/dc/terms/identifier | "doi:10.1016/0022-1759(94)90284-4"xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Frutiger S."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Frutiger S."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Hochstrasser D.F."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Hochstrasser D.F."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Hughes G.J."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Hughes G.J."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Paquet N."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Paquet N."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Pasquali C."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Pasquali C."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Tissot J.-D."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Tissot J.-D."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Schneider P."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Schneider P."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Schifferli J.A."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Schifferli J.A."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Spertini F."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Spertini F."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Clement F."xsd:string |
| http://purl.uniprot.org/citations/8034987 | http://purl.uniprot.org/core/author | "Clement F."xsd:string |