| http://purl.uniprot.org/citations/8110188 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8110188 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8110188 | http://www.w3.org/2000/01/rdf-schema#comment | "Phosphatidylinositol synthase (CDP-1,2-diacyl-sn-glycerol:myoinositol 3-phosphatidyltransferase, EC 2.7.8.11) was purified from the microsomal fraction of human placenta. The Triton X-100-extracted enzyme was purified 8300-fold over the microsomal fraction by affinity chromatography on CDP-diacylglycerol-Sepharose followed by ion-exchange chromatography on Mono Q. The purified enzyme had a molecular mass of 24,000 Da on SDS/PAGE. The enzyme had a pH optimum at 9.0, required Mn2+ or Mg2+, and was inhibited by Ca2+ and Zn2+. The Km for myo-inositol was determined to be 0.28 mM. Optimal activity was obtained at 0.2-0.4 mM CDP-diacylglycerol; higher concentrations of the lipid substrate inhibited the enzyme reaction. The enzyme was inhibited by nucleoside di- and tri-phosphates, Pi and PPi. CDP competitively inhibited the enzyme reaction with a Kis of 4 mM. The optimal temperature for the PtdIns synthase reaction was 50 degrees C."xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2970517"xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj2970517"xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/author | "Antonsson B.E."xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/author | "Antonsson B.E."xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/date | "1994"xsd:gYear |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/pages | "517-522"xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/pages | "517-522"xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/title | "Purification and characterization of phosphatidylinositol synthase from human placenta."xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/title | "Purification and characterization of phosphatidylinositol synthase from human placenta."xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/volume | "297"xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://purl.uniprot.org/core/volume | "297"xsd:string |
| http://purl.uniprot.org/citations/8110188 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8110188 |
| http://purl.uniprot.org/citations/8110188 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8110188 |
| http://purl.uniprot.org/citations/8110188 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8110188 |
| http://purl.uniprot.org/citations/8110188 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8110188 |
| http://purl.uniprot.org/uniprot/O14735 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/8110188 |
| http://purl.uniprot.org/uniprot/#_kb.O14735_up.isolatedFrom_tissue.799 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/8110188 |
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| http://purl.uniprot.org/uniprot/O14735#attribution-A998290583792ED0F7751B671FD75C6E | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8110188 |