| http://purl.uniprot.org/citations/8218242 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8218242 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8218242 | http://www.w3.org/2000/01/rdf-schema#comment | "Analysis of the primary protein structure of the human serotonin1B (5-HT1B) receptor reveals consensus sites for phosphorylation and a putative site for palmitoylation. To investigate these posttranslational modifications, we have expressed a c-myc epitope-tagged 5-HT1B (m5-HT1B) receptor in Sf9 cells. This strategy enabled receptors to be detected by immunoblot analysis and purified by immunoprecipitation using a monoclonal antibody, 9E10, specific for the c-myc epitope. Agonist radioligand [3H]5-HT binding studies showed that the expressed 5-HT1B and m5-HT1B receptors displayed the characteristic pharmacological profile of the neuronal 5-HT1B receptor. The expressed receptors displayed both high- and low-affinity states for [3H]5-HT, suggesting that the receptors were coupled to endogenous G-proteins. Indeed, agonist binding to the high-affinity receptor state was regulated in the presence of GTP gamma S, Gpp(NH)p, and pertussis toxin. [32P]ADP-ribosylation experiments identified a major approximately 41-kDa ADP-ribosylated protein present in Sf9 membranes that comigrated with partially purified bovine brain Gi alpha/G(o) alpha subunits. Measurements of adenylyl cyclase activity in membranes from cells expressing m5-HT1B receptors showed that serotonergic agonists mediated the inhibition of adenylyl cyclase activity with a rank order of potency comparable to their affinity constants. Immunoblot analysis of membranes prepared from cells expressing m5-HT1B receptors and photoaffinity labeling of the immunoprecipitated material revealed photolabeled species at approximately 95 and at approximately 42 kDa.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00094a032"xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00094a032"xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Bouvier M."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Bouvier M."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Caron M."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Caron M."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Dennis M."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Dennis M."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "George S.R."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "George S.R."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Ng G.Y.K."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Ng G.Y.K."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "O'Dowd B.F."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "O'Dowd B.F."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Zastawny R.L."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/author | "Zastawny R.L."xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/pages | "11727-11733"xsd:string |
| http://purl.uniprot.org/citations/8218242 | http://purl.uniprot.org/core/pages | "11727-11733"xsd:string |