| http://purl.uniprot.org/citations/8321221 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8321221 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8321221 | http://www.w3.org/2000/01/rdf-schema#comment | "AP-2 is a retinoic acid-inducible and developmentally regulated activator of transcription. We have cloned an alternative AP-2 transcript (AP-2B) from the human teratocarcinoma cell line PA-1, which encodes a protein differing in the C terminus from the previously isolated AP-2 protein (AP-2A). This protein contains the activation domain of AP-2 and part of the DNA binding domain but lacks the dimerization domain which is necessary for DNA binding. Analysis of overlapping genomic clones spanning the entire AP-2 gene proves that AP-2A and AP-2B transcripts are alternatively spliced from the same gene. Both transient and stable transfection experiments show that AP-2B inhibits AP-2 transactivator function, as measured by an AP-2-responsive chloramphenicol acetyltransferase reporter plasmid. Furthermore, constitutive AP-2B expression in PA-1 cells causes a retinoic acid-resistant phenotype, anchorage-independent growth in soft agar, and tumorigenicity in nude mice, in a fashion similar to transformation of these cells by oncogenes. To determine the mechanism by which AP-2B exerts its inhibitory function, we purified bacterially expressed AP-2A and AP-2B proteins. While bacterial AP-2B does not bind an AP-2 consensus site, it strongly inhibits binding of the endogenous AP-2 present in PA-1 cell nuclear extracts. However, DNA sequence-specific binding of bacterially expressed AP-2A cannot be inhibited by bacterially expressed AP-2B. Therefore, inhibition of AP-2 activity by the protein AP-2B may require an additional factor or modification supplied by nuclear extracts."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.13.7.4174-4185.1993"xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.13.7.4174-4185.1993"xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Bauer R."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Bauer R."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Buettner R."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Buettner R."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Glockshuber R."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Glockshuber R."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Imhof A."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Imhof A."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Kannan P."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Kannan P."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Tainsky M.A."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Tainsky M.A."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Van Dyke M.W."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Van Dyke M.W."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Yim S.O."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/author | "Yim S.O."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/8321221 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |