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| http://purl.uniprot.org/citations/8387502 | http://www.w3.org/2000/01/rdf-schema#comment | "Specific antisera were produced to peptides representing the carboxyl termini of three subtypes of phosphatidylinositol-specific phospholipase C (PIPLC) beta which have been identified by isolation of cDNAs (Kriz, R., Lin, L., Sultzman, L., Ellis, C., Heldin, C., Pawson, T., and Knopf, J. (1990) Ciba Found. Symp. 150, 112-127). Screening with the antisera indicates that PIPLC beta 3 is present in a variety of cell lines and rat tissues, whereas the distribution of PIPLC beta 1 and beta 2 is more restricted. A combination of conventional and immunoaffinity chromatographic techniques was used to purify PIPLC beta 1 and beta 3 from rat brain membranes. PIPLC beta 2 was purified from cytosol of HL60 cells. All three subtypes were activated by purified G protein alpha q/11 subunits with the following relative efficacies: PIPLC beta 3 > or = PIPLC beta 1 >> PIPLC beta 2. All three PIPLC subtypes were also activated by G protein beta gamma subunits with varying efficacies. The presence of beta gamma subunits depressed the ability of alpha q/11 to activate PIPLC beta 1 and beta 3 at low Mg2+ concentrations (1 mM). At higher concentrations of Mg2+ (2 mM or greater), activation of PIPLC beta 3, but not PIPLC beta 1, by beta gamma and alpha q/11 became additive. PIPLC beta 3 was activated by alpha q/11 even in the presence of a saturating concentration of beta gamma subunits. This indicates that there are separate sites for interaction of PIPLCs with G protein subunits and that this interaction differs depending on the enzyme subtype and the concentration of Mg2+."xsd:string |
| http://purl.uniprot.org/citations/8387502 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)98401-2"xsd:string |
| http://purl.uniprot.org/citations/8387502 | http://purl.uniprot.org/core/author | "Sternweis P.C."xsd:string |
| http://purl.uniprot.org/citations/8387502 | http://purl.uniprot.org/core/author | "Smrcka A.V."xsd:string |
| http://purl.uniprot.org/citations/8387502 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8387502 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/8387502 | http://purl.uniprot.org/core/pages | "9667-9674"xsd:string |
| http://purl.uniprot.org/citations/8387502 | http://purl.uniprot.org/core/title | "Regulation of purified subtypes of phosphatidylinositol-specific phospholipase C beta by G protein alpha and beta gamma subunits."xsd:string |
| http://purl.uniprot.org/citations/8387502 | http://purl.uniprot.org/core/volume | "268"xsd:string |
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