| http://purl.uniprot.org/citations/8399344 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8399344 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8399344 | http://www.w3.org/2000/01/rdf-schema#comment | "Alkyl-dihydroxyacetone phosphate synthase, the second enzyme involved in ether phospholipid biosynthesis from dihydroxyacetone phosphate and responsible for glycero-ether bond formation, has been purified from guinea-pig liver. Alkyl-dihydroxyacetone phosphate synthase was solubilized from a membrane fraction prepared from an enriched peroxisome fraction with Triton X-100 and potassium chloride. The solubilized enzyme was further purified by chromatography on QAE-Sephadex, Matrex Red, Phosphocellulose and Concanavalin A. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis alkyl-dihydroxyacetone phosphate synthase appears as a 65 kDa band. Chromatofocusing revealed an isoelectric point of pH 5.9 for the enzyme. The pH optimum of alkyl-dihydroxyacetone phosphate synthase was found to be between pH 7 and 8 in a 50 mM potassium phosphate buffer. The specific activity of the enzyme was estimated to be at least 350 nmol.min-1.mg-1, corresponding to a purification of at least 13,000-fold."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.org/dc/terms/identifier | "doi:10.1016/0005-2760(93)90070-p"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.org/dc/terms/identifier | "doi:10.1016/0005-2760(93)90070-p"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "van den Bosch H."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "van den Bosch H."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "Langeveld J."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "Langeveld J."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "Zomer A.W."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "Zomer A.W."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "de Weerd W.F."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/author | "de Weerd W.F."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/date | "1993"xsd:gYear |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/pages | "189-196"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/pages | "189-196"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/title | "Ether lipid synthesis: purification and identification of alkyl dihydroxyacetone phosphate synthase from guinea-pig liver."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/title | "Ether lipid synthesis: purification and identification of alkyl dihydroxyacetone phosphate synthase from guinea-pig liver."xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/volume | "1170"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://purl.uniprot.org/core/volume | "1170"xsd:string |
| http://purl.uniprot.org/citations/8399344 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8399344 |
| http://purl.uniprot.org/citations/8399344 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8399344 |