| http://purl.uniprot.org/citations/8774719 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8774719 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8774719 | http://www.w3.org/2000/01/rdf-schema#comment | "High-affinity progesterone-binding sites have been identified, characterized in and purified from porcine liver membranes. They were functionally solubilized by the non-denaturing zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (Chaps, 20 mM. detergent/protein mass ratio 4:1) at a yield of 75-80%. Using [3H]progesterone as radioligand, binding studies showed high-affinity and low-affinity binding sites in microsomal preparations with an apparent Kd2 of 11 nM and an apparent Kd2 of 286 nM. In solubilized fractions the high-affinity binding sites were present at an apparent Kd2 of 69 nM. In both preparations, progesterone binding was time-dependent, saturable, reversible, and showed a similar hierachy of affinities for related steroids. A purification scheme was developed based on anion-exchanger procedures. The purified fraction as identified by maximum specific progester-one-binding activity contained two major polypeptides of apparent molecular masses (SDS/PAGE) of 28 kDa and 56 kDa, respectively. Sequencing of both polypeptides showed an identical amino terminus without significant identity in the amino acid sequence to any known protein primary structure."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.0726u.x"xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.0726u.x"xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Meyer C."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Meyer C."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Schmid R."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Schmid R."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Scriba P.C."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Scriba P.C."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Wehling M."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/author | "Wehling M."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/pages | "726-731"xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/pages | "726-731"xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/title | "Purification and partial sequencing of high-affinity progesterone-binding site(s) from porcine liver membranes."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/title | "Purification and partial sequencing of high-affinity progesterone-binding site(s) from porcine liver membranes."xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/volume | "239"xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://purl.uniprot.org/core/volume | "239"xsd:string |
| http://purl.uniprot.org/citations/8774719 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8774719 |
| http://purl.uniprot.org/citations/8774719 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8774719 |