| http://purl.uniprot.org/citations/8774743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8774743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8774743 | http://www.w3.org/2000/01/rdf-schema#comment | "Molecular cloning of cDNA for a new regulatory subunit, designated p97, of the human 26S proteasome showed that the polypeptide consists of 908 amino acid residues with a calculated molecular mass of 100184 Da and an isoelectric point of 4.94. Computer analysis showed that p97 is very similar to type-1 tumor-necrosis-factor (TNF)-receptor-associated protein (TRAP)-2 and 55.11, both of which were identified recently as binding proteins of the cytoplasmic domain of type-1 TNF receptor by yeast two-hybrid screening. This finding suggests that the 26S proteasome might serve as a mediator molecule in the TNF signaling pathway in cells. Computer-assisted similarity analysis also revealed the high sequence similarity of p97 with a yeast protein whose function is yet unknown, the gene for which is here termed NAS1 (non-ATPase subunit 1). Disruption of NAS1 resulted in several phenotypes, including lethality and temperature-sensitive growth, depending on the genetic background of the cells used. The human p97 cDNA suppressed the growth defect of nas1 disruptant cells, when expressed from single-copy or multi-copy vectors, indicating that p97 is functionally equivalent to yeast Nas1p. Culturing of the temperature-sensitive nas1 cells at the restrictive temperature promoted the accumulation polyubiquitinated cellular proteins, implying that the 26S proteasome requires a functional Nas1p subunit for ubiquitin-dependent proteolysis. These results indicate that p97/Nas1p plays an important regulatory role in the function of the 26S proteasome."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.0912u.x"xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1996.0912u.x"xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Tanaka K."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Tanaka K."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Kato S."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Kato S."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Toh-e A."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Toh-e A."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Shimizu Y."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Shimizu Y."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Yamasaki M."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Yamasaki M."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Fujimuro M."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Fujimuro M."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Slaughter C.A."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Slaughter C.A."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "DeMartino G.N."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "DeMartino G.N."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Hendil K.B."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Hendil K.B."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Tanahashi N."xsd:string |
| http://purl.uniprot.org/citations/8774743 | http://purl.uniprot.org/core/author | "Tanahashi N."xsd:string |