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http://purl.uniprot.org/citations/8798417http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8798417http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8798417http://www.w3.org/2000/01/rdf-schema#comment"We have utilized the yeast two-hybrid system to identify proteins that interact with the cytoplasmic domain of the insulin receptor. We identified a human cDNA that is a splice variant of the human GRB10 homolog GRB-IR, which we term GRB10/IR-SV1 (for GRB10/GRB-IR splice variant 1). The protein encoded by the GRB10/IR-SV1 cDNA contains an SH2 domain and a pleckstrin homology domain. Cloning of a full-length human cDNA revealed a predicted coding sequence that was similar to the mouse GRB10 protein, although GRB10/IR-SV1 contained an 80-amino acid deletion. The GRB10/IR-SV1 cDNA is a splice variant of the GRB-IR cDNA such that GRB10/IR-SV1 contains an intact pleckstrin homology domain and a distinct amino terminus. The interaction of GRB10/IR-SV1 with the insulin receptor and the insulin-like growth factor I (IGF-I) receptor is mediated by the SH2 domain, and we show that glutathione S-transferase-SH2 domain fusion proteins interact specifically in vitro with the insulin receptor derived from mammalian cells. The GRB10/IR-SV1 SH2 domain also interacted with an approximately 135-kDa phosphoprotein from unstimulated cell lysates, an interaction that decreased after insulin stimulation. We present evidence that the GRB10/IR-SV1 protein plays a functional role in insulin and IGF-I signaling by showing that microinjection of an SH2 domain fusion protein inhibited insulin- and IGF-I-stimulated mitogenesis in fibroblasts, yet had no effect on mitogenesis induced by epidermal growth factor. Our findings suggest that GRB10/IR-SV1 may serve to positively link the insulin and IGF-I receptors to an uncharacterized mitogenic signaling pathway."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.org/dc/terms/identifier"doi:10.1074/jbc.271.37.22506"xsd:string
http://purl.uniprot.org/citations/8798417http://purl.org/dc/terms/identifier"doi:10.1074/jbc.271.37.22506"xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Hotta K."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Hotta K."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Rose D.W."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Rose D.W."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Olefsky J.M."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Olefsky J.M."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Pillay T.S."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Pillay T.S."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Gustafson T.A."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"Gustafson T.A."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"O'Neill T.J."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/author"O'Neill T.J."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/pages"22506-22513"xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/pages"22506-22513"xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/title"Interaction of a GRB-IR splice variant (a human GRB10 homolog) with the insulin and insulin-like growth factor I receptors. Evidence for a role in mitogenic signaling."xsd:string
http://purl.uniprot.org/citations/8798417http://purl.uniprot.org/core/title"Interaction of a GRB-IR splice variant (a human GRB10 homolog) with the insulin and insulin-like growth factor I receptors. Evidence for a role in mitogenic signaling."xsd:string
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