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http://purl.uniprot.org/citations/8836152http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8836152http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/8836152http://www.w3.org/2000/01/rdf-schema#comment"G alpha q and G alpha 11, members of the Gq family of G-proteins, transduce signals from receptors to the beta isoenzymes of phosphatidyl-inositol-specific phospholipase C (PI-PLC). The receptor specificity of these alpha subunits is unknown. G alpha q and G alpha 11 are ubiquitously expressed in tissues; however, there have been conflicting reports of the presence or absence of G alpha 11 protein in haematopoietic cells. Platelet thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptors activate PI-PLC via G alpha q, but the role of G alpha 11 is uncertain. To define their roles in platelet activation we studied G alpha q and G alpha 11 gene expression by immunotransfer blotting and by reverse transcription of mRNA followed by PCR (RT-PCR) and direct sequencing. An antiserum specific for mouse G alpha 11 failed to identify G alpha 11 in dog or human platelets or in dog liver, a tissue known to contain G alpha 11. RT-PCR performed with gene-specific primers demonstrated G alpha q mRNA, but not G alpha 11 mRNA, in normal human and mouse platelets and in thromboxane-sensitive and thromboxane-insensitive dog platelets. Studies of mouse and dog liver and human retina confirmed that the cDNA, primers and probes used could amplify and recognize G alpha 11 in other tissues. However, species-specific oligonucleotide primers and probes were essential to demonstrate G alpha 11, but not G alpha q, mRNA. Compared with mouse cDNA, dog and human G alpha 11 cDNA had twice as many nucleotide substitutions (approx. 12% compared with approx. 6%) as G alpha q, G alpha q mRNA was also found in mature erythrocytes but G alpha 11 mRNA was not identified, whereas both G alpha q and G alpha 11 mRNAs were found in bone marrow stem cells. Therefore G alpha 11 gene expression in haematopoietic cells is linked with cellular differentiation. The lack of G alpha 11 indicates that signal transduction from platelet TXA2/PGH2 receptors to PI-PLC occurs via G alpha q, and that G alpha 11 deficiency is not responsible for defective activation of PI-PLC in thromboxane-insensitive dog platelets. Despite the high degree of similarity that exists between G alpha q and G alpha 11, significantly greater species-specific variation in nucleotide sequence is present in G alpha 11 than in G alpha q. Cellular specificity and species specificity are important characteristics of these Gq family G-proteins."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.org/dc/terms/identifier"doi:10.1042/bj3181023"xsd:string
http://purl.uniprot.org/citations/8836152http://purl.org/dc/terms/identifier"doi:10.1042/bj3181023"xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/author"Dunlop P.C."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/author"Dunlop P.C."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/author"Johnson G.J."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/author"Johnson G.J."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/author"Leis L.A."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/author"Leis L.A."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/date"1996"xsd:gYear
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/name"Biochem. J."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/name"Biochem. J."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/pages"1023-1031"xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/pages"1023-1031"xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/title"Specificity of G alpha q and G alpha 11 gene expression in platelets and erythrocytes. Expressions of cellular differentiation and species differences."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/title"Specificity of G alpha q and G alpha 11 gene expression in platelets and erythrocytes. Expressions of cellular differentiation and species differences."xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/volume"318"xsd:string
http://purl.uniprot.org/citations/8836152http://purl.uniprot.org/core/volume"318"xsd:string
http://purl.uniprot.org/citations/8836152http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8836152
http://purl.uniprot.org/citations/8836152http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/8836152
http://purl.uniprot.org/citations/8836152http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8836152
http://purl.uniprot.org/citations/8836152http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/8836152
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