| http://purl.uniprot.org/citations/8992506 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8992506 | http://www.w3.org/2000/01/rdf-schema#comment | "Human epidermal cells produce a wide range of cytokines, including those characteristic of Th2-like responses such as interleukin (IL)-4 and IL-10. As well, keratinocytes have recently been shown to produce Th1-like cytokines such as IL-12. Exposure to UVB has profound effects on the skin and systemic immune system, which is in part mediated by secretion of tumor necrosis factor (TNF)-alpha by epidermal cells. Because IL-12 induces production of TNF-alpha by certain cells of the immune system, we sought to determine whether UVB is an inducer of IL-12 gene expression in epidermal cells. Human epidermal cells were exposed to UVB radiation in vivo, isolated by suction blister technique and trypsinization and transcription of the IL-12 p35 and p40 chains was examined by RT-PCR. We found the p35 chain of IL-12 to be constitutively expressed and the p40 chain inducible by UVB irradiation. Because epidermis consists of a heterogenous cell population with distinct cytokine repertoires, we sought to determine the cellular source of the IL-12 message after UVB exposure. After depleting UVB-exposed epidermal cells for DR+ cells, no reduction in the IL-12 activity was detected, suggesting that keratinocytes are a source of IL-12 transcripts in UVB-exposed human epidermis. This was supported by the up-regulation of IL-12 p40 transcripts in UV-irradiated cultured keratinocytes that were devoid of DR+ cells. Up-regulation of IL-12 p40 gene expression by UVB as demonstrated here, taken together with the finding that keratinocytes also up-regulate IL-10 transcription, suggests that there is a complex interplay between Th1- and Th2-like epidermis-derived cytokines following exposure to UVB."xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1751-1097.1996.tb09642.x"xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/author | "Mahanty S."xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/author | "Blauvelt A."xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/author | "Enk C.D."xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/author | "Katz S.I."xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/date | "1996"xsd:gYear |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/name | "Photochem Photobiol"xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/pages | "854-859"xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/title | "UVB induces IL-12 transcription in human keratinocytes in vivo and in vitro."xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://purl.uniprot.org/core/volume | "63"xsd:string |
| http://purl.uniprot.org/citations/8992506 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/8992506 |
| http://purl.uniprot.org/citations/8992506 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/8992506 |
| http://purl.uniprot.org/uniprot/P29459#attribution-D33172147EFC3DD366B6EBE1228DF053 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8992506 |
| http://purl.uniprot.org/uniprot/P29460#attribution-D33172147EFC3DD366B6EBE1228DF053 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/8992506 |