| http://purl.uniprot.org/citations/8999859 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8999859 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/8999859 | http://www.w3.org/2000/01/rdf-schema#comment | "Replication factor C (RF-C) is a heteropentameric protein essential for DNA replication and repair. It is a molecular matchmaker required for loading of proliferating cell nuclear antigen (PCNA) onto double-stranded DNA and, thus, for PCNA-dependent DNA elongation by DNA polymerases delta and epsilon. To elucidate the mode of RF-C binding to the PCNA clamp, modified forms of human PCNA were used that could be 32P-labeled in vitro either at the C or the N terminus. Using a kinase protection assay, we show that the heteropentameric calf thymus RF-C was able to protect the C-terminal region but not the N-terminal region of human PCNA from phosphorylation, suggesting that RF-C interacts with the PCNA face at which the C termini are located (C-side). A similar protection profile was obtained with the recently identified PCNA binding region (residues 478-712), but not with the DNA binding region (residues 366-477), of the human RF-C large subunit (Fotedar, R., Mossi, R., Fitzgerald, P., Rousselle, T., Maga, G., Brickner, H., Messner, H., Khastilba, S., Hübscher, U., and Fotedar, A., (1996) EMBO J., 15, 4423-4433). Furthermore, we show that the RF-C 36 kDa subunit of human RF-C could interact independently with the C-side of PCNA. The RF-C large subunit from a third species, namely Drosophila melanogaster, interacted similarly with the modified human PCNA, indicating that the interaction between RF-C and PCNA is conserved through eukaryotic evolution."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.3.1769"xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.3.1769"xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Jonsson Z.O."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Jonsson Z.O."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Huebscher U."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Huebscher U."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Hardin S.H."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Hardin S.H."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Allen B.L."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Allen B.L."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Mossi R."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/author | "Mossi R."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/pages | "1769-1776"xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/pages | "1769-1776"xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/title | "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/title | "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen."xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/8999859 | http://purl.uniprot.org/core/volume | "272"xsd:string |