| http://purl.uniprot.org/citations/9060999 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9060999 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9060999 | http://www.w3.org/2000/01/rdf-schema#comment | "CaMK-II (the (type II) multifunctional Ca2+/CaM-dependent protein kinase) has been implicated in diverse neuronal and non-neuronal functions, including cell growth control. CaMKII expression was evaluated in a variety of human tumor cell lines using RT-PCR (reverse transcriptase coupled polymerase chain reaction). PCR primers which flanked the CaMK-II variable domain were used so that all possible variants of the four mammalian CaMK-II genes (alpha, beta, gamma and delta) could be identified. 8 distinct CaMK-II isozymes were identified from human mammary tumor and neuroblastoma cell cDNA, each of which represented a variant of beta, gamma or delta CaMK-II. They included 2 beta isozymes (beta e, beta 'e), 4 gamma isozymes (gamma B, gamma C, gamma G, gamma H) and 2 delta isozymes (delta C, delta E) This is the first report of human beta and delta CaMK-II sequences. A panel of human cell types was then screened for these CaMK-II isozymes. As expected, cerebral cortex predominately expressed alpha, beta and delta A CaMK-II. In contrast, tumor cells, including those of neuronal origin, expressed an entirely different spectrum of CaMK-II isozymes than adult neuronal tissue. Tumor cells of diverse tissue origin uniformly lacked alpha CaMK-II and expressed 1-2 beta isozymes, at least 3 gamma isozymes and 1-2 delta isozymes. When compared to undifferentiated fibroblasts, beta e, beta'e, gamma G and gamma H were preferentially expressed in tumor cells. CaMK-II immunoblots also indicated that neuroblastoma and mammary tumor cells express isozymes of CaMK-II not present in their non-transformed cell or tissue counterpart. The identification of these new, potential tumor-specific CaMK-II variants supports previous indications that CaMK-II plays a role in growth control. In addition, these results provide insight into both splice variant switching and variable domain structural similarities among all CaMK-II isozymes."xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-4889(96)00141-3"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-4889(96)00141-3"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.org/dc/terms/identifier | "doi:10.1016/S0167-4889(96)00141-3"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/author | "Tombes R.M."xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/author | "Tombes R.M."xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/author | "Krystal G.W."xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/author | "Krystal G.W."xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/pages | "281-292"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/pages | "281-292"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/title | "Identification of novel human tumor cell-specific CaMK-II variants."xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/title | "Identification of novel human tumor cell-specific CaMK-II variants."xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/volume | "1355"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://purl.uniprot.org/core/volume | "1355"xsd:string |
| http://purl.uniprot.org/citations/9060999 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9060999 |
| http://purl.uniprot.org/citations/9060999 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9060999 |
| http://purl.uniprot.org/citations/9060999 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9060999 |
| http://purl.uniprot.org/citations/9060999 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9060999 |
| http://purl.uniprot.org/uniprot/Q92991 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9060999 |