| http://purl.uniprot.org/citations/9169443 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9169443 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9169443 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9169443 | http://www.w3.org/2000/01/rdf-schema#comment | "A human liver carboxylesterase (hCE-2) that catalyzes the hydrolysis of the benzoyl group of cocaine and the acetyl groups of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine was purified from human liver. The purified enzyme exhibited a single band on SDS-polyacrylamide gel electrophoresis with a subunit mass of approximately 60 kDa. The native enzyme was monomeric. The isoelectric point of hCE-2 was approximately 4.9. Treatment with endoglycosidase H caused an increase in electrophoretic mobility indicating that the liver carboxylesterase was a glycoprotein of the high mannose type. The complete cDNA nucleotide sequence was determined. The authenticity of the cDNA was confirmed by a perfect sequence match of 78 amino acids derived from the hCE-2 purified from human liver. The mature 533-amino acid enzyme encoded by this cDNA shared highest sequence identity with the rabbit liver carboxylesterase form 2 (73%) and the hamster liver carboxylesterase AT51p (67%). Carboxylesterases with high sequence identity to hCE-2 have not been reported in mouse and rat liver. hCE-2 exhibited different drug ester substrate specificity from the human liver carboxylesterase called hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 had higher catalytic efficiencies for hydrolysis of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine and greater inhibition by eserine than hCE-1. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.23.14769"xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.23.14769"xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Dean R.A."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Dean R.A."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Bosron W.F."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Bosron W.F."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Kedishvili N.Y."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Kedishvili N.Y."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Abraham T.L."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Abraham T.L."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Brzezinski M.R."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Brzezinski M.R."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Pindel E.V."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/author | "Pindel E.V."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9169443 | http://purl.uniprot.org/core/pages | "14769-14775"xsd:string |