| http://purl.uniprot.org/citations/9312046 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9312046 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9312046 | http://www.w3.org/2000/01/rdf-schema#comment | "Binding of macrophage colony stimulating factor (M-CSF) to its receptor (Fms) induces dimerization and activation of the tyrosine kinase domain of the receptor, resulting in autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins that relay growth and development signals. To determine whether a distinct signaling pathway is responsible for the Fms differentiation signal versus the growth signal, we sought new molecules involved in Fms signaling by performing a two-hybrid screen in yeast using the autophosphorylated cytoplasmic domain of the wild-type Fms receptor as bait. Clones containing SH2 domains of phospholipase C-gamma2 (PLC-gamma2) were frequently isolated and shown to interact with phosphorylated Tyr721 of the Fms receptor, which is also the binding site of the p85 subunit of phosphatidylinositol 3-kinase (PI3-kinase). At variance with previous reports, M-CSF induced rapid and transient tyrosine phosphorylation of PLC-gamma2 in myeloid FDC-P1 cells and this activation required the activity of the PI3-kinase pathway. The Fms Y721F mutation strongly decreased this activation. Moreover, the Fms Y807F mutation decreased both binding and phosphorylation of PLC-gamma2 but not that of p85. Since the Fms Y807F mutation abrogates the differentiation signal when expressed in FDC-P1 cells and since this phenotype could be reproduced by a specific inhibitor of PLC-gamma, we propose that a balance between the activities of PLC-gamma2 and PI3-kinase in response to M-CSF is required for cell differentiation."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/16.19.5880"xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/16.19.5880"xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Bourette R.P."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Bourette R.P."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Choi J.L."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Choi J.L."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Myles G.M."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Myles G.M."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Rohrschneider L.R."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/author | "Rohrschneider L.R."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/pages | "5880-5893"xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/pages | "5880-5893"xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/title | "Sequential activation of phoshatidylinositol 3-kinase and phospholipase C-gamma2 by the M-CSF receptor is necessary for differentiation signaling."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/title | "Sequential activation of phoshatidylinositol 3-kinase and phospholipase C-gamma2 by the M-CSF receptor is necessary for differentiation signaling."xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/9312046 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9312046 |
| http://purl.uniprot.org/citations/9312046 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9312046 |