| http://purl.uniprot.org/citations/9328340 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9328340 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9328340 | http://www.w3.org/2000/01/rdf-schema#comment | "A GC-rich oligonucleotide containing an estrogen responsive element (ERE) half-site from the heat shock protein 27 (Hsp 27) gene promoter (-105 to -84) [ie. GGGCGGG(N)10GGTCA; Sp1(N)10ERE] forms a complex with the Sp1 and estrogen receptor (ER) proteins. Moreover, promoter-reporter constructs containing this sequence (-108 to -84 or -108 to +23) are also estrogen-responsive. Mutation of the ERE half-site in the Hsp 27-derived oligonucleotides did not result in loss of estrogen responsiveness in transient transfection studies, suggesting that estrogen inducibility was mediated through the Sp1-DNA motif. Gel mobility shift assays using 32P-labeled wild type and ERE mutant Sp1(N)10ERE and consensus Sp1 oligonucleotides showed that Sp1 protein formed a DNA-protein complex with all three nucleotides, and the intensities of retarded bands were enhanced by coincubation with wild type ER and 11C-ER, which does not contain the DNA-binding domain. ER mutants in which N-terminal (19C-ER) and C-terminal (15C-ER) regions were deleted did not enhance Sp1-DNA binding or hormone-induced transactivation of GC-rich promoter-reporter constructs in ER-negative MDA-MB-231 cells, whereas both wild type and 11C-ER restored inducibility. Immunoprecipitation studies also confirmed that the Sp1 and ER proteins physically interact. The interaction of the Sp1 and ER proteins and the resulting enhanced Sp1-DNA binding is observed in the presence or absence of estrogen (hormone-independent), whereas transactivation of promoter-reporter constructs is estrogen-dependent. Thus, the results illustrate a new estrogen-dependent transactivation pathway that involves ER-protein interactions and is ERE-independent."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.org/dc/terms/identifier | "doi:10.1210/mend.11.11.9916"xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.org/dc/terms/identifier | "doi:10.1210/mend.11.11.9916"xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Hoivik D."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Hoivik D."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Safe S."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Safe S."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Porter W."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Porter W."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Saville B."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/author | "Saville B."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/name | "Mol. Endocrinol."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/name | "Mol. Endocrinol."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/pages | "1569-1580"xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/pages | "1569-1580"xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/title | "Functional synergy between the transcription factor Sp1 and the estrogen receptor."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/title | "Functional synergy between the transcription factor Sp1 and the estrogen receptor."xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/9328340 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9328340 |
| http://purl.uniprot.org/citations/9328340 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9328340 |