| http://purl.uniprot.org/citations/9388232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9388232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9388232 | http://www.w3.org/2000/01/rdf-schema#comment | "Bad, an inducer of programmed cell death, was recently isolated from a mouse cDNA library by its ability to bind to the anti-apoptotic protein BCL-2. Sequence analysis suggested that Bad was a member of the BCL-2 gene family that encodes both inducers and inhibitors of programmed cell death. To further analyze the role of BAD in the network of homo- and heterodimers formed by the BCL-2 family, we have cloned the human homologue of BAD and assessed its biological activity and its interactions with wild type and mutant BCL-2 family proteins. Our results indicate that the human BAD protein, like its mouse homologue, is able to induce apoptosis when transfected into mammalian cells. Furthermore, in yeast two-hybrid assays as well as quantitative in vitro interaction assays, human Bad interacted with BCL-2 and BCL-XL. Sequence alignments of human BAD revealed the presence of a BH-3 homology domain as seen in other BCL-2 family proteins. Peptides derived from this domain were able to completely inhibit the dimerization of BAD with BCL-XL. Thus, as previously shown for BAX, BAK, BCL-2, and BCL-XL, the BH3 domain of BAD is required for its dimerization with other BCL-2 family proteins. BAD was further analyzed for its ability to bind to various mutants of BCL-2 and BCL-XL that have lost the ability to bind BAX and BAK, some of which retain biological activity and some of which do not. Surprisingly, all of the mutated BCL-2 and BCL-XL proteins analyzed strongly interacted with human BAD. Our data thus indicate that mutations in BCL-2 and BCL-XL can differentially affect the heterodimeric binding of different death-promoting proteins and have implications concerning the relationship between heterodimerization and biological activity."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.49.30866"xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.49.30866"xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Chang S."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Chang S."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Chang J."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Chang J."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Ottilie S."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Ottilie S."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Fritz L.C."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Fritz L.C."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Oltersdorf T."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Oltersdorf T."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Wilson G."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Wilson G."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Diaz J.-L."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Diaz J.-L."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Horne W."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Horne W."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Weeks S."xsd:string |
| http://purl.uniprot.org/citations/9388232 | http://purl.uniprot.org/core/author | "Weeks S."xsd:string |