Results
Your Query
▶
▶
| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/9393974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9393974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9393974 | http://www.w3.org/2000/01/rdf-schema#comment | "Using differential display, we identified an mRNA that is markedly down-regulated in cell line 6A/SB1, derived from a fibrosarcoma formed in an athymic mouse following injection of carcinogen-transformed MSU-1.1 cells. The nontumorigenic parental cell strain, MSU-1.1, expresses high levels of this mRNA. Sequencing of the corresponding cDNA fragment revealed that it corresponded to an expressed sequence tag, which ultimately led to its identification as the fibulin-1D gene. Fibulin-1 is a cysteine-rich, calcium-binding extracellular matrix and plasma protein, which has four isoforms, A-D, derived from alternative splicing. Northern and Western blotting analysis of 16 cell lines established from tumors formed in athymic mice by MSU-1.1-derived cell strains independently transformed in culture showed that 44% exhibited low level or lack of expression of fibulin-1D mRNA and protein. In a similar analysis of 15 malignant cell lines derived from patients, 80% showed low level or no expression. To study the role of fibulin-1D in transformation, we transfected 6A/SB1 cells and a human fibrosarcoma-derived cell line (SHAC) with a fibulin-1D cDNA expression construct. Transfectants displaying high levels of fibulin-1D were isolated and characterized. Elevated expression of fibulin-1D led to reduced ability to form colonies in soft agar and reduced invasive potential as tested in a matrigel in vitro invasion assay. Furthermore, expression of fibulin-1D resulted in a markedly extended latency in tumor formation in athymic mice. These results indicate that low expression of fibulin-1D plays a role in tumor formation and invasion."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1201385"xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1201385"xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Argraves W.S."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Argraves W.S."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Tran H."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Tran H."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Qing J."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Qing J."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Dunstan R.W."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Dunstan R.W."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Maher V.M."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "Maher V.M."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "McCormick J.J."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/author | "McCormick J.J."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/pages | "2159-2168"xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/pages | "2159-2168"xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/title | "Suppression of anchorage-independent growth and matrigel invasion and delayed tumor formation by elevated expression of fibulin-1D in human fibrosarcoma-derived cell lines."xsd:string |
| http://purl.uniprot.org/citations/9393974 | http://purl.uniprot.org/core/title | "Suppression of anchorage-independent growth and matrigel invasion and delayed tumor formation by elevated expression of fibulin-1D in human fibrosarcoma-derived cell lines."xsd:string |