| http://purl.uniprot.org/citations/9422762 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9422762 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9422762 | http://www.w3.org/2000/01/rdf-schema#comment | "Cell adhesion kinase beta (CAKbeta/PYK2) is the second protein-tyrosine kinase of the focal adhesion kinase subfamily. We identified a cDNA that encodes a CAKbeta-binding protein. This cDNA clone encodes the human homologue of Hic-5, the cDNA of which was cloned in 1994 as transforming growth factor beta1- and hydrogen peroxide-inducible mRNA. We found that Hic-5 exclusively localized at focal adhesions in a rat fibroblast line, WFB. This localization of Hic-5 was confirmed in WFB cells expressing Myc-tagged Hic-5. The amino acid sequence of Hic-5 is highly similar to that of paxillin in the four LD motifs as well as in the four contiguous LIM domains. The Hic-5 N-terminal domain directly associated in vitro with the extreme C-terminal region (residue 801 to the end) of CAKbeta. CAKbeta was coimmunoprecipitated with Hic-5 from the WFB cell lysate. The coimmunoprecipitation of CAKbeta with Hic-5 was markedly inhibited by the addition of the extreme C-terminal region of CAKbeta. Coimmunoprecipitation of Hic-5 with CAKbeta, which was shown in COS-7 cells doubly transfected with cDNA constructs of CAKbeta and Myc-tagged Hic-5, was lost when the CAKbeta amino acid residues 741-903 were deleted. Hic-5 was tyrosine-phosphorylated in Src-transformed 3Y1 cells and in cells treated with pervanadate. Hic-5 associated with CAKbeta was selectively tyrosine-phosphorylated in WFB cells exposed to hypertonic osmotic stress. These results indicate that Hic-5 is a paxillin-related component of focal adhesions and binds to CAKbeta, implying possible involvement of Hic-5 in the downstream signaling of CAKbeta."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.2.1003"xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.2.1003"xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Ohba T."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Ohba T."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Sasaki T."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Sasaki T."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Takahashi S."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Takahashi S."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Sasaki H."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Sasaki H."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Suzuki R."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Suzuki R."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Ishino M."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Ishino M."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Aoto H."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Aoto H."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Matsuya M."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Matsuya M."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Mitaka T."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Mitaka T."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Nagura K."xsd:string |
| http://purl.uniprot.org/citations/9422762 | http://purl.uniprot.org/core/author | "Nagura K."xsd:string |