http://purl.uniprot.org/citations/9574564 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9574564 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9574564 | http://www.w3.org/2000/01/rdf-schema#comment | "A snake venom-like protease isolated by a differential display screen between normal and osteoarthritis (OA)-affected cartilage (designated as cSVP) has a cDNA sequence identical to TNF-alpha convertase enzyme (TACE). TACE shows the presence of an unknown prodomain, a cysteine switch, a catalytic domain, a zinc binding region, a disintegrin region, an EGF-like domain, a transmembrane domain, and a unique cytoplasmic region. A TACE construct harboring the signal + prodomain + catalytic region (TACE-SPCdeltaDETCy), expressed in baculovirus could cleave preferentially (approximately 12-fold) the TNF-specific peptide over the matrix metalloproteases peptide in vitro. This recombinant protein also cleaved the natural substrate GST-ProTNF-alpha to TNF-alpha (17 kDa) in vitro. The mRNA for TACE, which is broadly distributed and differentially expressed in a variety of human tissues, is up-regulated in arthritis-affected cartilage, but not normal cartilage. OA-affected cartilage also expressed TNF-alpha mRNA that was not detected in normal cartilage. The OA-affected cartilage (in explant assays) spontaneously released TNF-alpha and IL-8 in ex vivo conditions. Addition of TNF-alphaR fused to IgG Fc fragment (TNF-alphaR:Fc) in the presence or absence of soluble IL-1R (with which it acted additively) significantly attenuated the spontaneous/autocrine release of articular IL-8 in this assay. These experiments demonstrate a functional paracrine/autocrine role of TNF-alpha in OA-affected cartilage that may depend, in part, on up-regulated levels of chondrocyte-derived TACE."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Patel I.R."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Patel I.R."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Abagyan R.A."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Abagyan R.A."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Abramson S.B."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Abramson S.B."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Amin A.R."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Amin A.R."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Attur M.G."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Attur M.G."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Patel R.N."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Patel R.N."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Stuchin S.A."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/author | "Stuchin S.A."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/pages | "4570-4579"xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/pages | "4570-4579"xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/title | "TNF-alpha convertase enzyme from human arthritis-affected cartilage: isolation of cDNA by differential display, expression of the active enzyme, and regulation of TNF-alpha."xsd:string |
http://purl.uniprot.org/citations/9574564 | http://purl.uniprot.org/core/title | "TNF-alpha convertase enzyme from human arthritis-affected cartilage: isolation of cDNA by differential display, expression of the active enzyme, and regulation of TNF-alpha."xsd:string |