| http://purl.uniprot.org/citations/9582328 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9582328 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9582328 | http://www.w3.org/2000/01/rdf-schema#comment | "After receiving an external stimulus Dictyostelium amoebae are able to rearrange their actin cytoskeleton within seconds, and phosphorylation is a prime candidate for quick modification of cytoskeletal components. We isolated a kinase from cytosolic extracts that specifically phosphorylated severin, a Ca2+-dependent F-actin fragmenting protein. In gel filtration chromatography severin kinase eluted with a molecular mass of about 300 kDa and contained a 62-kDa component whose autophosphorylation caused a mobility shift in SDS-polyacrylamide gel electrophoresis and stimulated phosphorylation of severin. Severin kinase activity could be specifically precipitated with antibodies raised against the 62-kDa polypeptide. Phosphorylation of severin was strongly reduced in the presence of Ca2+, indicating additional regulation at the substrate level. Peptide sequencing and cloning of the cDNA demonstrated that the 62-kDa protein belongs to the Ste20p- or p21-activated protein kinase family. It is most closely related to the germinal center kinase subfamily with its N-terminal positioned catalytic domain followed by a presumptive regulatory domain at the C terminus. The presence of a Ste20-like severin kinase in Dictyostelium suggests a direct signal transduction from the plasma membrane to the cytoskeleton by phosphorylation of actin-binding proteins."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.21.12952"xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.21.12952"xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Eichinger L."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Eichinger L."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Schleicher M."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Schleicher M."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Eckerskorn C."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Eckerskorn C."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Baehler M."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Baehler M."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Dietz M."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/author | "Dietz M."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/pages | "12952-12959"xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/pages | "12952-12959"xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/title | "Characterization and cloning of a Dictyostelium Ste20-like protein kinase that phosphorylates the actin-binding protein severin."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/title | "Characterization and cloning of a Dictyostelium Ste20-like protein kinase that phosphorylates the actin-binding protein severin."xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9582328 | http://purl.uniprot.org/core/volume | "273"xsd:string |