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http://purl.uniprot.org/citations/9597769http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9597769http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9597769http://www.w3.org/2000/01/rdf-schema#comment"The potential of electrospray mass spectrometry (ESMS) for the sequencing of glycopeptides was evaluated using quadrupole time-of-flight (QTOF) technology in the MS/MS mode. The location of O-glycosylation sites was possible in the positive ion (+) mode by detection of prominent y- and b-fragment ions from the underivatized TAP25-2 [T1APPAHGVT9S10APDT14RPAPGS20T21APPA], an overlapping sequence of MUC1 tandem repeats which had been glycosylated in vitro by two GalNAc residues in the positions T9 and T21. The high mass resolution and accuracy of QTOF-(+)ESMS allowed reliable structural assignments. The reduced complexity of the fragment spectra and the higher signal-to-noise ratio render QTOF-(+)ESMS an alternative mass spectrometric approach to the identification of O-glycosylation sites when compared with sequencing by post-source decay matrix-assisted laser desorption/ionization MS. Diagnostic ions from the N-terminus in the b-series offered direct evidence, which was supported by indirect evidence from the C-terminus ions of the y-series. The higher glycosylated GalNAc2-substituted fragments were mainly observed as multiply ionized species."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.org/dc/terms/identifier"doi:10.1002/(sici)1096-9888(199804)33:4<358::aid-jms642>3.0.co;2-3"xsd:string
http://purl.uniprot.org/citations/9597769http://purl.org/dc/terms/identifier"doi:10.1002/(sici)1096-9888(199804)33:4<358::aid-jms642>3.0.co;2-3"xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Green B.N."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Green B.N."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Peter-Katalinic J."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Peter-Katalinic J."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Bateman R."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Bateman R."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Hanisch F.G."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/author"Hanisch F.G."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/name"J. Mass Spectrom."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/name"J. Mass Spectrom."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/pages"358-362"xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/pages"358-362"xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/title"Localization of O-glycosylation sites of MUC1 tandem repeats by QTOF ESI mass spectrometry."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/title"Localization of O-glycosylation sites of MUC1 tandem repeats by QTOF ESI mass spectrometry."xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/volume"33"xsd:string
http://purl.uniprot.org/citations/9597769http://purl.uniprot.org/core/volume"33"xsd:string
http://purl.uniprot.org/citations/9597769http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9597769
http://purl.uniprot.org/citations/9597769http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9597769