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http://purl.uniprot.org/citations/9710597http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9710597http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9710597http://www.w3.org/2000/01/rdf-schema#comment"Using the DNA-binding domain of androgen receptor (AR) as a bait in a yeast two-hybrid screening, we have identified a small nuclear RING finger protein, termed SNURF, that interacts with AR in a hormone-dependent fashion in both yeast and mammalian cells. Physical interaction between AR and SNURF was demonstrated by coimmunoprecipitation from cell extracts and by protein-protein affinity chromatography. Rat SNURF is a highly hydrophilic protein consisting of 194 amino acid residues and comprising a consensus C3HC4 zinc finger (RING) structure in the C-terminal region and a bipartite nuclear localization signal near the N terminus. Immunohistochemical experiments indicated that SNURF is a nuclear protein. SNURF mRNA is expressed in a variety of human and rat tissues. Overexpression of SNURF in cultured mammalian cells enhanced not only androgen, glucocorticoid, and progesterone receptor-dependent transactivation but also basal transcription from steroid-regulated promoters. Mutation of two of the potential Zn2+ coordinating cysteines to serines in the RING finger completely abolished the ability of SNURF to enhance basal transcription, whereas its ability to activate steroid receptor-dependent transcription was maintained, suggesting that there are separate domains in SNURF that mediate interactions with different regulatory factors. SNURF is capable of interacting in vitro with the TATA-binding protein, and the RING finger domain is needed for this interaction. Collectively, we have identified and characterized a ubiquitously expressed RING finger protein, SNURF, that may function as a bridging factor and regulate steroid receptor-dependent transcription by a mechanism different from those of previously identified coactivator or integrator proteins."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.org/dc/terms/identifier"doi:10.1128/mcb.18.9.5128"xsd:string
http://purl.uniprot.org/citations/9710597http://purl.org/dc/terms/identifier"doi:10.1128/mcb.18.9.5128"xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Palvimo J.J."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Palvimo J.J."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Karvonen U."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Karvonen U."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Moilanen A.-M."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Moilanen A.-M."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Poukka H."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Poukka H."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Janne O.A."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Janne O.A."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Hakli M."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/author"Hakli M."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/name"Mol. Cell. Biol."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/name"Mol. Cell. Biol."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/pages"5128-5139"xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/pages"5128-5139"xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/title"Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription."xsd:string
http://purl.uniprot.org/citations/9710597http://purl.uniprot.org/core/title"Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription."xsd:string
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