| http://purl.uniprot.org/citations/9724530 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9724530 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9724530 | http://www.w3.org/2000/01/rdf-schema#comment | "The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was determined by chemical methods using partial reduction with tris(2-carboxyethyl)phosphine under acidic conditions, followed by direct alkylation with N-ethylmaleimide or fluorescein-5-maleimide. Partial reduction and alkylation provided several forms of AGRP that were modified in a stepwise fashion. The resulting proteins were characterized by peptide mapping, sequence analysis, and mass spectrometry, showing that AGRP contained a highly reducible disulfide bond, C85-C109, followed by less reactive ones, C90-C97, C74-C88, C67-C82, and C81-C99, respectively. The chemically defined disulfide connectivity of the recombinant human AGRP was homologous to that of omega-agatoxin IVB except for an additional disulfide bond, C85-C109."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi981082v"xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi981082v"xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Haniu M."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Haniu M."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Bures E.J."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Bures E.J."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Chow D.T."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Chow D.T."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Hui J.O."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Hui J.O."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Katta V."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Katta V."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Rohde M.F."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Rohde M.F."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Rosenfeld R.D."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Rosenfeld R.D."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Stark K.L."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Stark K.L."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Young Y."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Young Y."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Zeni L."xsd:string |
| http://purl.uniprot.org/citations/9724530 | http://purl.uniprot.org/core/author | "Zeni L."xsd:string |