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http://purl.uniprot.org/citations/9753425http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9753425http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9753425http://www.w3.org/2000/01/rdf-schema#comment"

Background

Tec is a member of the recently emerging subfamily among nonreceptor protein-tyrosine kinases (PTKs). Although many members of this family have been shown to be involved in a wide range of cytokine-mediated signalling systems, the molecular mechanism by which they exert in vivo effects remains obscure. To gain insights into the downstream pathways of Tec, we here looked for Tec-interacting proteins (TIPs) by using the yeast two-hybrid screening.

Results

One of TIPs turned out to be Grb10/GrbIR, which carries one pleckstrin homology domain and one Src homology 2 domain. Grb10/GrbIR was known to bind receptor PTKs in a ligand-dependent fashion, but not to be phosphorylated on tyrosine residues. In a transient expression system in human kidney 293 cells, however, Grb10/GrbIR becomes profoundly tyrosine-phosphorylated by Tec, but not by Syk, Jak2 or insulin receptor. We also reveal that expression of Grb10/GrbIR suppresses the cytokine-driven and Tec-driven activation of the c-fos promoter.

Conclusion

Our results indicate a novel role of Grb10/GrbIR as an effector molecule to a subset of nonreceptor PTKs."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.1998.00201.x"xsd:string
http://purl.uniprot.org/citations/9753425http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.1998.00201.x"xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Kasuga M."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Kasuga M."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Ozawa K."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Ozawa K."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Inazawa J."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Inazawa J."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Shimada K."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Shimada K."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Yamashita Y."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Yamashita Y."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Kajigaya S."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Kajigaya S."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Miyazato A."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Miyazato A."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Ogawa W."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Ogawa W."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Mano H."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Mano H."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Ohya K."xsd:string
http://purl.uniprot.org/citations/9753425http://purl.uniprot.org/core/author"Ohya K."xsd:string
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