| http://purl.uniprot.org/citations/9857026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9857026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9857026 | http://www.w3.org/2000/01/rdf-schema#comment | "The Rho-related small GTPases are critical elements involved in regulation of signal transduction cascades from extracellular stimuli to cell nucleus and cytoskeleton. The Dbl-like guanine nucleotide exchange factors (GEF) have been implicated in direct activation of these GTPases. Here we have identified a new member of the Dbl family, GEF-H1, by screening a human HeLa cell cDNA library. GEF-H1 encodes a 100-kDa protein containing the conserved structural array of a Dbl homology domain in tandem with a pleckstrin homology domain and is most closely related to the lfc oncogene, but additionally it contains a unique coiled-coil domain at the carboxyl terminus. Biochemical analysis reveals that GEF-H1 is capable of stimulating guanine nucleotide exchange of Rac and Rho but is inactive toward Cdc42, TC10, or Ras. Moreover, GEF-H1 binds to Rac and Rho proteins in both the GDP- and guanosine 5'-3-O-(thio)triphosphate-bound states without detectable affinity for Cdc42 or Ras. Immunofluorescence reveals that GEF-H1 colocalizes with microtubules through the carboxyl-terminal coiled-coil domain. Overexpression of GEF-H1 in COS-7 cells results in induction of membrane ruffles. These results suggest that GEF-H1 may have a direct role in activation of Rac and/or Rho and in bringing the activated GTPase to specific target sites such as microtubules."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.52.34954"xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.52.34954"xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Li R."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Li R."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Ren Y."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Ren Y."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Zheng Y."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Zheng Y."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Busch H."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/author | "Busch H."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/pages | "34954-34960"xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/pages | "34954-34960"xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/title | "Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/title | "Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases."xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9857026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9857026 |
| http://purl.uniprot.org/citations/9857026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9857026 |