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http://purl.uniprot.org/citations/9889151http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9889151http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9889151http://www.w3.org/2000/01/rdf-schema#comment"

Background

RANTES is a CC-type chemokine protein that acts as a chemoattractant for several kinds of leukocytes, playing an important pro-inflammatory role. Entry of human immunodeficiency virus-1 (HIV-1) into cells depends on the chemokine receptor CCR5. RANTES binds CCR5 and inhibits HIV-1 entry into peripheral blood cells. Interaction with chemokine receptors involves a distinct set of residues at the amino terminus of RANTES. This finding was utilized in the development of a chemically modified aminooxypentane derivative of RANTES, AOP-RANTES, that was originally produced from the recombinant protein using semisynthetic methods.

Results

AOP-RANTES has been produced by a novel total chemical synthesis that provides efficient, direct access to large amounts of this anti-HIV protein analog. The crystal structure of chemically synthesized AOP-RANTES has been solved and refined at 1.6 A resolution. The protein is a dimer, with the amino-terminal pentane oxime moiety clearly defined.

Conclusions

Total chemical synthesis of AOP-RANTES provides a convenient method of producing the multi-milligram quantities of this protein needed to investigate the molecular basis of receptor binding and antiviral activity. This work provides the first truly high-resolution structure of a RANTES protein, although the structure of RANTES was known from previous nuclear magnetic resonance (NMR) determinations."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.org/dc/terms/identifier"doi:10.1016/s1074-5521(99)80019-2"xsd:string
http://purl.uniprot.org/citations/9889151http://purl.org/dc/terms/identifier"doi:10.1016/s1074-5521(99)80019-2"xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Lubkowski J."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Lubkowski J."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Wlodawer A."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Wlodawer A."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Thompson D.A."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Thompson D.A."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Barlow P.N."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Barlow P.N."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Hoover D."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Hoover D."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Picard L."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Picard L."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Kent S.B."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Kent S.B."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Wilken J."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"Wilken J."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"McSparron H."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/author"McSparron H."xsd:string
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/date"1999"xsd:gYear
http://purl.uniprot.org/citations/9889151http://purl.uniprot.org/core/date"1999"xsd:gYear
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