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Entry version 35 (31 Jul 2019)
Sequence version 1 (01 Oct 2014)
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Protein

Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1

Gene

GSCOC_T00021252001

Organism
Coffea canephora (Robusta coffee)
Status
Unreviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (GSCOC_T00041548001)
  2. Probable methylthioribulose-1-phosphate dehydratase (GSCOC_T00042264001)
  3. no protein annotated in this organism
  4. Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (GSCOC_T00021252001)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (GSCOC_T00026136001), 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (GSCOC_T00026137001)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei121Substrate; for methylthioribulose-1-phosphate dehydratase activityUniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi139Zinc; via tele nitrogenUniRule annotation1
Metal bindingi141Zinc; via tele nitrogenUniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei164Proton donor/acceptor; for methylthioribulose-1-phosphate dehydratase activityUniRule annotation1
Metal bindingi214Zinc; via tele nitrogenUniRule annotation1
Metal bindingi296MagnesiumUniRule annotation1
Metal bindingi298Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei465Substrate; for enolase-phosphatase activityUniRule annotation1
Metal bindingi491MagnesiumUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolaseUniRule annotation, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotation
Biological processAmino-acid biosynthesis, Methionine biosynthesisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00904;UER00877

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1UniRule annotation
Including the following 2 domains:
Methylthioribulose-1-phosphate dehydrataseUniRule annotation (EC:4.2.1.109UniRule annotation)
Short name:
MTRu-1-P dehydrataseUniRule annotation
Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:GSCOC_T00021252001Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCoffea canephora (Robusta coffee)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri49390 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeIxoroideaeGardenieae complexBertiereae - Coffeeae cladeCoffeeaeCoffea
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000295252 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini38 – 241Aldolase_IIInterPro annotationAdd BLAST204

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 249Methylthioribulose-1-phosphate dehydrataseUniRule annotationAdd BLAST249
Regioni293 – 532Enolase-phosphatase E1UniRule annotationAdd BLAST240
Regioni431 – 432Substrate binding; for enolase-phosphatase activityUniRule annotation2

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the C-terminal section; belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.UniRule annotation
In the N-terminal section; belongs to the aldolase class II family. MtnB subfamily.UniRule annotation

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01629 HAD_EP, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.225.10, 1 hit
3.40.50.1000, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_03116 Salvage_MtnB_euk, 1 hit
MF_03118 Salvage_MtnBC, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001303 Aldolase_II/adducin_N
IPR036409 Aldolase_II/adducin_N_sf
IPR023943 Enolase-ppase_E1
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017714 MethylthioRu-1-P_deHdtase_MtnB
IPR027505 MtnB_viridiplantae
IPR027514 Salvage_MtnB_euk

The PANTHER Classification System

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PANTHERi
PTHR10640 PTHR10640, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00596 Aldolase_II, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01007 Aldolase_II, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53639 SSF53639, 1 hit
SSF56784 SSF56784, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01691 enolase-ppase, 1 hit
TIGR03328 salvage_mtnB, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A0A068UBJ2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAALATPLA LNGAVKMANS AITSQAYLEG SRVKETKALI AELCRHFYNL
60 70 80 90 100
GWVSGTGGSI TIKVHDDSVP KPQQLIVMSP SGVQKERMVE EDMYLLSPEG
110 120 130 140 150
SILSAPLAKP YPHKPPKCSD CGPLFLKAYD MRNAGAVIHS HGMESCLVTM
160 170 180 190 200
INPFSKEFRI THMEMIKGIQ GHGYYDELVV PIIENTAHER ELTDSLAEAI
210 220 230 240 250
KAYPKTTAVL VRNHGIYVWG DNWISAKTQA ECYHYLFDAA IKLHQLGLDW
260 270 280 290 300
STPSHGPIHS VNGALGNVRS ANTPVKARKL ASDNGIEPLR RCIVLDIEGT
310 320 330 340 350
TTPISFVTDV LFPYARESVG KHLDATYDTE ETQDDIKLLR AQVQEDLEKG
360 370 380 390 400
IVGAVPIPDD DAGKQEVIAA LVANVEAMIK ADRKITALKQ LQGHVWRTGF
410 420 430 440 450
DNSELEATVF DDVPEALERW HALGIKVYIY SSGSRLAQRL LFGNTNYGDL
460 470 480 490 500
RKYLCGYFDT TVGNKRETKS YLEITESVGV DKPSEILFVT DVYQEATAAK
510 520 530
AAGLNVIISI RPGNGPLPEN HGFRTVKSFS EI
Length:532
Mass (Da):58,468
Last modified:October 1, 2014 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB7A1240B8BACB48E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
HG739103 Genomic DNA Translation: CDP05930.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HG739103 Genomic DNA Translation: CDP05930.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Enzyme and pathway databases

UniPathwayiUPA00904;UER00877

Family and domain databases

CDDicd01629 HAD_EP, 1 hit
Gene3Di3.40.225.10, 1 hit
3.40.50.1000, 1 hit
HAMAPiMF_03116 Salvage_MtnB_euk, 1 hit
MF_03118 Salvage_MtnBC, 1 hit
InterProiView protein in InterPro
IPR001303 Aldolase_II/adducin_N
IPR036409 Aldolase_II/adducin_N_sf
IPR023943 Enolase-ppase_E1
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017714 MethylthioRu-1-P_deHdtase_MtnB
IPR027505 MtnB_viridiplantae
IPR027514 Salvage_MtnB_euk
PANTHERiPTHR10640 PTHR10640, 1 hit
PfamiView protein in Pfam
PF00596 Aldolase_II, 1 hit
SMARTiView protein in SMART
SM01007 Aldolase_II, 1 hit
SUPFAMiSSF53639 SSF53639, 1 hit
SSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01691 enolase-ppase, 1 hit
TIGR03328 salvage_mtnB, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiA0A068UBJ2_COFCA
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A068UBJ2
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: October 1, 2014
Last sequence update: October 1, 2014
Last modified: July 31, 2019
This is version 35 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health

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