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Entry version 155 (18 Sep 2019)
Sequence version 1 (13 Aug 1987)
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Protein

Alpha-ketoglutarate-dependent dioxygenase AlkB

Gene

alkB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).6 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Fe2+PROSITE-ProRule annotation4 PublicationsNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei69Substrate2 Publications1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi131Iron; catalyticPROSITE-ProRule annotation1 Publication1
Metal bindingi133Iron; catalyticPROSITE-ProRule annotation1 Publication1
Binding sitei135Substrate2 Publications1
Binding sitei161Substrate2 Publications1
Metal bindingi187Iron; catalyticPROSITE-ProRule annotation1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processDNA damage, DNA repair
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG10037-MONOMER
ECOL316407:JW2200-MONOMER
MetaCyc:EG10037-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.14.11.33 2026

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase AlkB (EC:1.14.11.33)
Alternative name(s):
Alkylated DNA repair protein AlkB
DNA oxidative demethylase AlkB
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:alkB
Synonyms:aidD
Ordered Locus Names:b2212, JW2200
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG10037 alkB

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi51T → A: Slighly reduced activity towards single-stranded DNA containing 1-methyladenine. Reduces affinity for undamaged DNA. 1 Publication1
Mutagenesisi69W → A: Abolishes activity towards single-stranded DNA containing 1-methyladenine. 1 Publication1
Mutagenesisi76Y → A: Reduces affinity for damaged DNA and activity towards single-stranded DNA containing 1-methyladenine. 1 Publication1
Mutagenesisi135D → A: Abolishes activity towards single-stranded DNA containing 1-methyladenine. Alters substrate specificity, so that the enzyme gains activity towards single-stranded DNA containing 1-methylguanine. 1 Publication1
Mutagenesisi161R → A: No effect on enzyme activity. Decreases affinity for damaged DNA. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000666651 – 216Alpha-ketoglutarate-dependent dioxygenase AlkBAdd BLAST216

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P05050

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P05050

PRoteomics IDEntifications database

More...
PRIDEi
P05050

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4261918, 29 interactors
851049, 1 interactor

Database of interacting proteins

More...
DIPi
DIP-9085N

Protein interaction database and analysis system

More...
IntActi
P05050, 12 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b2212

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P05050

Database of comparative protein structure models

More...
ModBasei
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P05050

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini113 – 213Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni76 – 78Substrate binding3
Regioni120 – 122Alpha-ketoglutarate binding3
Regioni204 – 210Alpha-ketoglutarate binding7

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the alkB family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105CF1 Bacteria
COG3145 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000122276

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P05050

KEGG Orthology (KO)

More...
KOi
K03919

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P05050

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.120.590, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR004574 Alkb
IPR027450 AlkB-like
IPR037151 AlkB-like_sf
IPR005123 Oxoglu/Fe-dep_dioxygenase

The PANTHER Classification System

More...
PANTHERi
PTHR16557 PTHR16557, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13532 2OG-FeII_Oxy_2, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00568 alkb, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P05050-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLDLFADAEP WQEPLAAGAV ILRRFAFNAA EQLIRDINDV ASQSPFRQMV
60 70 80 90 100
TPGGYTMSVA MTNCGHLGWT THRQGYLYSP IDPQTNKPWP AMPQSFHNLC
110 120 130 140 150
QRAATAAGYP DFQPDACLIN RYAPGAKLSL HQDKDEPDLR APIVSVSLGL
160 170 180 190 200
PAIFQFGGLK RNDPLKRLLL EHGDVVVWGG ESRLFYHGIQ PLKAGFHPLT
210
IDCRYNLTFR QAGKKE
Length:216
Mass (Da):24,076
Last modified:August 13, 1987 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i494FE1E70EABD278
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
J02607 Genomic DNA Translation: AAA23416.1
U00008 Genomic DNA Translation: AAA16409.1
U00096 Genomic DNA Translation: AAC75272.1
AP009048 Genomic DNA Translation: BAA15995.1
M10315 Genomic DNA Translation: AAA23414.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A24605 BVECKB

NCBI Reference Sequences

More...
RefSeqi
NP_416716.1, NC_000913.3
WP_000884971.1, NZ_SSZK01000030.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75272; AAC75272; b2212
BAA15995; BAA15995; BAA15995

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
946708

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW2200
eco:b2212

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.23

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02607 Genomic DNA Translation: AAA23416.1
U00008 Genomic DNA Translation: AAA16409.1
U00096 Genomic DNA Translation: AAC75272.1
AP009048 Genomic DNA Translation: BAA15995.1
M10315 Genomic DNA Translation: AAA23414.1
PIRiA24605 BVECKB
RefSeqiNP_416716.1, NC_000913.3
WP_000884971.1, NZ_SSZK01000030.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FD8X-ray2.30A12-216[»]
2FDFX-ray2.10A12-216[»]
2FDGX-ray2.20A12-216[»]
2FDHX-ray2.10A12-216[»]
2FDIX-ray1.80A12-216[»]
2FDJX-ray2.10A12-216[»]
2FDKX-ray2.30A12-216[»]
3BI3X-ray1.90A13-213[»]
3BIEX-ray1.68A13-214[»]
3BKZX-ray1.65A14-214[»]
3I2OX-ray1.70A12-216[»]
3I3MX-ray1.50A12-216[»]
3I3QX-ray1.40A/B12-216[»]
3I49X-ray1.60A12-216[»]
3KHBX-ray2.90A/B1-216[»]
3KHCX-ray2.20A/B1-216[»]
3O1MX-ray1.75A12-216[»]
3O1OX-ray1.92A12-216[»]
3O1PX-ray1.51A12-216[»]
3O1RX-ray1.77A12-216[»]
3O1SX-ray1.58A12-216[»]
3O1TX-ray1.48A12-216[»]
3O1UX-ray1.54A12-216[»]
3O1VX-ray1.90A12-216[»]
3T3YX-ray2.00A12-216[»]
3T4HX-ray1.65B12-216[»]
3T4VX-ray1.73A12-216[»]
4JHTX-ray1.18A12-216[»]
4NIDX-ray1.58A12-216[»]
4NIGX-ray1.52A12-216[»]
4NIHX-ray1.37A12-216[»]
4NIIX-ray1.62A12-216[»]
4RFRX-ray1.50B14-216[»]
4ZHNX-ray1.33A12-216[»]
SMRiP05050
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4261918, 29 interactors
851049, 1 interactor
DIPiDIP-9085N
IntActiP05050, 12 interactors
STRINGi511145.b2212

Proteomic databases

jPOSTiP05050
PaxDbiP05050
PRIDEiP05050

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75272; AAC75272; b2212
BAA15995; BAA15995; BAA15995
GeneIDi946708
KEGGiecj:JW2200
eco:b2212
PATRICifig|1411691.4.peg.23

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0036
EcoGeneiEG10037 alkB

Phylogenomic databases

eggNOGiENOG4105CF1 Bacteria
COG3145 LUCA
HOGENOMiHOG000122276
InParanoidiP05050
KOiK03919
PhylomeDBiP05050

Enzyme and pathway databases

BioCyciEcoCyc:EG10037-MONOMER
ECOL316407:JW2200-MONOMER
MetaCyc:EG10037-MONOMER
BRENDAi1.14.11.33 2026

Miscellaneous databases

EvolutionaryTraceiP05050

Protein Ontology

More...
PROi
PR:P05050

Family and domain databases

Gene3Di2.60.120.590, 1 hit
InterProiView protein in InterPro
IPR004574 Alkb
IPR027450 AlkB-like
IPR037151 AlkB-like_sf
IPR005123 Oxoglu/Fe-dep_dioxygenase
PANTHERiPTHR16557 PTHR16557, 1 hit
PfamiView protein in Pfam
PF13532 2OG-FeII_Oxy_2, 1 hit
TIGRFAMsiTIGR00568 alkb, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiALKB_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P05050
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 18, 2019
This is version 155 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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