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Entry version 162 (18 Sep 2019)
Sequence version 2 (23 May 2003)
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Protein

Mediator of RNA polymerase II transcription subunit 14

Gene

RGR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-32223-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 14
Alternative name(s):
Glucose repression regulatory protein 1
Mediator complex subunit 14
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:RGR1
Synonyms:MED14
Ordered Locus Names:YLR071C
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XII

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YLR071C

Saccharomyces Genome Database

More...
SGDi
S000004061 RGR1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000963622 – 1082Mediator of RNA polymerase II transcription subunit 14Add BLAST1081

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylthreonineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei1036PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P19263

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P19263

PRoteomics IDEntifications database

More...
PRIDEi
P19263

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P19263

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
31345, 371 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-3226 Core mediator complex

Database of interacting proteins

More...
DIPi
DIP-1562N

Protein interaction database and analysis system

More...
IntActi
P19263, 38 interactors

Molecular INTeraction database

More...
MINTi
P19263

STRING: functional protein association networks

More...
STRINGi
4932.YLR071C

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P19263

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Mediator complex subunit 14 family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000113520

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P19263

KEGG Orthology (KO)

More...
KOi
K15156

Identification of Orthologs from Complete Genome Data

More...
OMAi
PHVEINQ

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013947 Mediator_Med14

The PANTHER Classification System

More...
PANTHERi
PTHR12809 PTHR12809, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08638 Med14, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P19263-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT
60 70 80 90 100
DPETTATQKE SLEMVPKDTS AATMTSAPPP ALPHVEINQV SLALVIRNLT
110 120 130 140 150
VFTMKELAQY MKTNVHTQAN EPNSAKKIRF LQLIIFLRTQ FLKLYVLVKW
160 170 180 190 200
TRTIKQNNFH VLIDLLNWFR TTNMNVNNCI WALKSSLNSM TNAKLPNVDL
210 220 230 240 250
VTALEVLSLG RPNLPTHNFK LSGVSNSMDM VDGMAKVPIG LILQRLKDLN
260 270 280 290 300
LTVSIKIALM NIPKPLNSYH IKNGRIYFTV PNEFEIQLST VNRQSPLFFV
310 320 330 340 350
DLKLLFNTEA EQTVSAVTEA TSTNGDSENN EENSSSNGNN LPLNKPRLEK
360 370 380 390 400
LINEILLKSN DPLLSLYNFL HKYVLTLQLY MVHREFLKLA NGGKFSKSNL
410 420 430 440 450
IHNYDSKKST ITVRYWLNGK MDSKGKITIG IQRTTESLIL KWDNQSASRA
460 470 480 490 500
KNMPVIYNNI VSNIEGILDE IMFNHARIIR SELLARDIFQ EDEENSDVLL
510 520 530 540 550
FQLPTTCVSM APIQLKIDLL SGQFYFRNPT PLLSNYASKI NRAEGPEELA
560 570 580 590 600
RILQQLKLDK IIHVLTTMFE NTGWSCSRII KIDKPIRTQV NTGGESVVKK
610 620 630 640 650
EDNKYAIAGN STTNSDVSLL LQRDLFIRLP HWPLNWYLIL SIISSKTSCV
660 670 680 690 700
VEKRIGKIVS QRGKWNLKYL DNSNVMTVKL ESITYQKIMI LQRTILNRII
710 720 730 740 750
NHMLIDSLNQ LEIRNKICSS EMINEQKLPQ YIIQGSNTND NISIITLELE
760 770 780 790 800
SFLEGSKALN SILESSMFLR IDYSNSQIRL YAKFKRNTMM IQCQIDKLYI
810 820 830 840 850
HFVQEEPLAF YLEESFTNLG IIVQYLTKFR QKLMQLVVLT DVVERLHKNF
860 870 880 890 900
ESENFKIIAL QPNEISFKYL SNNDEDDKDC TIKISTNDDS IKNLTVQLSP
910 920 930 940 950
SNPQHIIQPF LDNSKMDYHF IFSYLQFTSS LFKALKVILN ERGGKFHESG
960 970 980 990 1000
SQYSTMVNIG LHNLNEYQIV YYNPQAGTKI TICIELKTVL HNGRDKIQFH
1010 1020 1030 1040 1050
IHFADVAHIT TKSPAYPMMH QVRNQVFMLD TKRLGTPESV KPANASHAIR
1060 1070 1080
LGNGVACDPS EIEPILMEIH NILKVDSNSS SS
Length:1,082
Mass (Da):123,358
Last modified:May 23, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i96277001829DB539
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti46G → S in BAA14104 (PubMed:2196447).Curated1
Sequence conflicti51D → V in BAA14104 (PubMed:2196447).Curated1
Sequence conflicti56A → T in BAA14104 (PubMed:2196447).Curated1
Sequence conflicti285E → V in BAA14104 (PubMed:2196447).Curated1
Sequence conflicti746T → A in BAA14104 (PubMed:2196447).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D90051 Genomic DNA Translation: BAA14104.1
Z73243 Genomic DNA Translation: CAA97628.1
AY692942 Genomic DNA Translation: AAT92961.1
BK006945 Genomic DNA Translation: DAA09388.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S64903

NCBI Reference Sequences

More...
RefSeqi
NP_013172.1, NM_001181958.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YLR071C_mRNA; YLR071C; YLR071C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
850760

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YLR071C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90051 Genomic DNA Translation: BAA14104.1
Z73243 Genomic DNA Translation: CAA97628.1
AY692942 Genomic DNA Translation: AAT92961.1
BK006945 Genomic DNA Translation: DAA09388.1
PIRiS64903
RefSeqiNP_013172.1, NM_001181958.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5OQMelectron microscopy5.80l1-745[»]
SMRiP19263
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi31345, 371 interactors
ComplexPortaliCPX-3226 Core mediator complex
DIPiDIP-1562N
IntActiP19263, 38 interactors
MINTiP19263
STRINGi4932.YLR071C

PTM databases

iPTMnetiP19263

Proteomic databases

MaxQBiP19263
PaxDbiP19263
PRIDEiP19263

Genome annotation databases

EnsemblFungiiYLR071C_mRNA; YLR071C; YLR071C
GeneIDi850760
KEGGisce:YLR071C

Organism-specific databases

EuPathDBiFungiDB:YLR071C
SGDiS000004061 RGR1

Phylogenomic databases

HOGENOMiHOG000113520
InParanoidiP19263
KOiK15156
OMAiPHVEINQ

Enzyme and pathway databases

BioCyciYEAST:G3O-32223-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P19263

Family and domain databases

InterProiView protein in InterPro
IPR013947 Mediator_Med14
PANTHERiPTHR12809 PTHR12809, 1 hit
PfamiView protein in Pfam
PF08638 Med14, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMED14_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P19263
Secondary accession number(s): D6VY72, Q07999
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 23, 2003
Last modified: September 18, 2019
This is version 162 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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