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Entry version 158 (08 May 2019)
Sequence version 1 (01 Oct 1996)
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Protein

Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Gene

ALG12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man7GlcNAc2) required for protein glycosylation.2 Publications

Miscellaneous

Present with 3550 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER3O-256
YEAST:MONOMER3O-256

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.4.1.260 984

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00378

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
GT22 Glycosyltransferase Family 22

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase (EC:2.4.1.2601 Publication)
Alternative name(s):
Asparagine-linked glycosylation protein 12
Dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase
Extracellular mutant protein 39
Mannosyltransferase ALG12
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ALG12
Synonyms:ECM39
Ordered Locus Names:YNR030W
ORF Names:N3265
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XIV

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YNR030W

Saccharomyces Genome Database

More...
SGDi
S000005313 ALG12

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 2LumenalSequence analysis2
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Topological domaini24 – 61CytoplasmicSequence analysisAdd BLAST38
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Topological domaini83 – 89LumenalSequence analysis7
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Topological domaini111 – 136CytoplasmicSequence analysisAdd BLAST26
Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
Topological domaini158 – 178LumenalSequence analysisAdd BLAST21
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Topological domaini200 – 202CytoplasmicSequence analysis3
Transmembranei203 – 223HelicalSequence analysisAdd BLAST21
Topological domaini224 – 227LumenalSequence analysis4
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
Topological domaini249 – 275CytoplasmicSequence analysisAdd BLAST27
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 303LumenalSequence analysis7
Transmembranei304 – 324HelicalSequence analysisAdd BLAST21
Topological domaini325 – 331CytoplasmicSequence analysis7
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 365LumenalSequence analysisAdd BLAST13
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 417CytoplasmicSequence analysisAdd BLAST31
Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 551LumenalSequence analysisAdd BLAST113

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002157861 – 551Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferaseAdd BLAST551

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P53730

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P53730

PRoteomics IDEntifications database

More...
PRIDEi
P53730

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
35854, 113 interactors

Database of interacting proteins

More...
DIPi
DIP-4259N

Protein interaction database and analysis system

More...
IntActi
P53730, 23 interactors

Molecular INTeraction database

More...
MINTi
P53730

STRING: functional protein association networks

More...
STRINGi
4932.YNR030W

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycosyltransferase 22 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree

More...
GeneTreei
ENSGT00950000183090

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000248044

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P53730

KEGG Orthology (KO)

More...
KOi
K03847

Identification of Orthologs from Complete Genome Data

More...
OMAi
VDSFFWQ

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR039485 ALG12
IPR005599 GPI_mannosylTrfase

The PANTHER Classification System

More...
PANTHERi
PTHR22760 PTHR22760, 1 hit
PTHR22760:SF1 PTHR22760:SF1, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03901 Glyco_transf_22, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P53730-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRWSVLDTVL LTVISFHLIQ APFTKVEESF NIQAIHDILT YSVFDISQYD
60 70 80 90 100
HLKFPGVVPR TFVGAVIIAM LSRPYLYLSS LIQTSRPTSI DVQLVVRGIV
110 120 130 140 150
GLTNGLSFIY LKNCLQDMFD EITEKKKEEN EDKDIYIYDS AGTWFLLFLI
160 170 180 190 200
GSFHLMFYST RTLPNFVMTL PLTNVALGWV LLGRYNAAIF LSALVAIVFR
210 220 230 240 250
LEVSALSAGI ALFSVIFKKI SLFDAIKFGI FGLGLGSAIS ITVDSYFWQE
260 270 280 290 300
WCLPEVDGFL FNVVAGYASK WGVEPVTAYF THYLRMMFMP PTVLLLNYFG
310 320 330 340 350
YKLAPAKLKI VSLASLFHII VLSFQPHKEW RFIIYAVPSI MLLGATGAAH
360 370 380 390 400
LWENMKVKKI TNVLCLAILP LSIMTSFFIS MAFLYISRMN YPGGEALTSF
410 420 430 440 450
NDMIVEKNIT NATVHISIPP CMTGVTLFGE LNYGVYGINY DKTENTTLLQ
460 470 480 490 500
EMWPSFDFLI THEPTASQLP FENKTTNHWE LVNTTKMFTG FDPTYIKNFV
510 520 530 540 550
FQERVNVLSL LKQIIFDKTP TVFLKELTAN SIVKSDVFFT YKRIKQDEKT

D
Length:551
Mass (Da):62,672
Last modified:October 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB08A94BBF260502F
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z71645 Genomic DNA Translation: CAA96310.1
AY692956 Genomic DNA Translation: AAT92975.1
BK006947 Genomic DNA Translation: DAA10570.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S63361

NCBI Reference Sequences

More...
RefSeqi
NP_014427.1, NM_001183207.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YNR030W_mRNA; YNR030W_mRNA; YNR030W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
855764

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YNR030W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71645 Genomic DNA Translation: CAA96310.1
AY692956 Genomic DNA Translation: AAT92975.1
BK006947 Genomic DNA Translation: DAA10570.1
PIRiS63361
RefSeqiNP_014427.1, NM_001183207.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGridi35854, 113 interactors
DIPiDIP-4259N
IntActiP53730, 23 interactors
MINTiP53730
STRINGi4932.YNR030W

Protein family/group databases

CAZyiGT22 Glycosyltransferase Family 22

Proteomic databases

MaxQBiP53730
PaxDbiP53730
PRIDEiP53730

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR030W_mRNA; YNR030W_mRNA; YNR030W
GeneIDi855764
KEGGisce:YNR030W

Organism-specific databases

EuPathDBiFungiDB:YNR030W
SGDiS000005313 ALG12

Phylogenomic databases

GeneTreeiENSGT00950000183090
HOGENOMiHOG000248044
InParanoidiP53730
KOiK03847
OMAiVDSFFWQ

Enzyme and pathway databases

UniPathwayiUPA00378
BioCyciMetaCyc:MONOMER3O-256
YEAST:MONOMER3O-256
BRENDAi2.4.1.260 984
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P53730

Family and domain databases

InterProiView protein in InterPro
IPR039485 ALG12
IPR005599 GPI_mannosylTrfase
PANTHERiPTHR22760 PTHR22760, 1 hit
PTHR22760:SF1 PTHR22760:SF1, 1 hit
PfamiView protein in Pfam
PF03901 Glyco_transf_22, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiALG12_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P53730
Secondary accession number(s): D6W1K4
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 8, 2019
This is version 158 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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