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Entry version 205 (18 Sep 2019)
Sequence version 3 (09 Feb 2010)
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Protein

Afadin

Gene

AFDN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton (PubMed:11024295). May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions (PubMed:30463011).By similarity2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-418990 Adherens junctions interactions

SignaLink: a signaling pathway resource with multi-layered regulatory networks

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SignaLinki
P55196

SIGNOR Signaling Network Open Resource

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SIGNORi
P55196

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Afadin
Alternative name(s):
ALL1-fused gene from chromosome 6 protein
Short name:
Protein AF-6
Afadin adherens junction formation factorImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:AFDNImported
Synonyms:AF6, MLLT4
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 6

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:7137 AFDN

Online Mendelian Inheritance in Man (OMIM)

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MIMi
159559 gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_P55196

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

A chromosomal aberration involving AFDN is associated with acute leukemias. Translocation t(6;11)(q27;q23) with KMT2A/MLL1. The result is a rogue activator protein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei26Breakpoint for translocation to form KMT2A/MLL1-AFDN1

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNET

More...
DisGeNETi
4301

Open Targets

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OpenTargetsi
ENSG00000130396

Chemistry databases

Drug and drug target database

More...
DrugBanki
DB08574 (5R)-2-SULFANYL-5-[4-(TRIFLUOROMETHYL)BENZYL]-1,3-THIAZOL-4-ONE

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
AFDN

Domain mapping of disease mutations (DMDM)

More...
DMDMi
288558835

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002159181 – 1824AfadinAdd BLAST1824

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei216PhosphoserineCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei256PhosphoserineCombined sources1
Modified residuei391PhosphoserineBy similarity1
Modified residuei424PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei557PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1
Modified residuei589PhosphoserineCombined sources1
Modified residuei655PhosphoserineCombined sources1
Modified residuei1083PhosphoserineCombined sources1
Modified residuei1107PhosphoserineCombined sources1
Modified residuei1126PhosphoserineBy similarity1
Modified residuei1140PhosphoserineBy similarity1
Modified residuei1143PhosphoserineCombined sources1
Modified residuei1172PhosphoserineBy similarity1
Modified residuei1173PhosphoserineCombined sources1
Modified residuei1182PhosphoserineCombined sources1
Modified residuei1199PhosphoserineCombined sources1
Modified residuei1211PhosphothreonineCombined sources1
Modified residuei1232PhosphothreonineCombined sources1
Modified residuei1238PhosphoserineCombined sources1
Modified residuei1275PhosphoserineCombined sources1
Modified residuei1328PhosphoserineCombined sources1
Modified residuei1330PhosphothreonineCombined sources1
Modified residuei1501PhosphoserineCombined sources1
Modified residuei1512PhosphoserineCombined sources1
Modified residuei1696PhosphoserineCombined sources1
Modified residuei1721PhosphoserineCombined sources1
Modified residuei1774PhosphoserineBy similarity1
Modified residuei1779PhosphoserineCombined sources1
Modified residuei1799PhosphoserineCombined sources1
Modified residuei1807N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
P55196

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
P55196

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
P55196

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P55196

PeptideAtlas

More...
PeptideAtlasi
P55196

PRoteomics IDEntifications database

More...
PRIDEi
P55196

ProteomicsDB human proteome resource

More...
ProteomicsDBi
56796 [P55196-4]
56797 [P55196-1]
56798 [P55196-2]
56799 [P55196-3]
56800 [P55196-5]
56801 [P55196-6]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P55196

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
P55196

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSG00000130396 Expressed in 203 organ(s), highest expression level in oviduct epithelium

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
P55196 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
P55196 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
CAB013496
HPA030212
HPA030213
HPA030214
HPA030215
HPA049868

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

Interacts with F-actin, nectin and NECTIN3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin.

Interacts with ZO-1 and occludin, but probably in an indirect manner.

Interacts with RIT1 and RIT2 (By similarity).

Interacts with NRXN1 and BCR.

Interacts with ADAM10; the interaction locks ADAM10 at adherens junctions following ADAM10 recruitment to adherens junctions by TSPAN33 (PubMed:30463011).

By similarity3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
110447, 109 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
P55196

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

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ELMi
P55196

Protein interaction database and analysis system

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IntActi
P55196, 43 interactors

Molecular INTeraction database

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MINTi
P55196

STRING: functional protein association networks

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STRINGi
9606.ENSP00000375960

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11824
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P55196

Database of comparative protein structure models

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ModBasei
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P55196

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini39 – 133Ras-associating 1PROSITE-ProRule annotationAdd BLAST95
Domaini246 – 348Ras-associating 2PROSITE-ProRule annotationAdd BLAST103
Domaini426 – 492FHAAdd BLAST67
Domaini668 – 908DilutePROSITE-ProRule annotationAdd BLAST241
Domaini1007 – 1093PDZPROSITE-ProRule annotationAdd BLAST87

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili146 – 185Sequence analysisAdd BLAST40
Coiled coili1408 – 1448Sequence analysisAdd BLAST41
Coiled coili1523 – 1667Sequence analysisAdd BLAST145

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi163 – 175Glu/Lys-richAdd BLAST13
Compositional biasi1346 – 1392Pro-richAdd BLAST47
Compositional biasi1578 – 1588Asp/Glu-rich (acidic)Add BLAST11
Compositional biasi1678 – 1708Pro-richAdd BLAST31

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminal domain interacts with F-actin.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1892 Eukaryota
ENOG410XR0S LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00940000155237

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
P55196

KEGG Orthology (KO)

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KOi
K05702

Identification of Orthologs from Complete Genome Data

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OMAi
TVYTAKF

Database of Orthologous Groups

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OrthoDBi
23029at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
P55196

TreeFam database of animal gene trees

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TreeFami
TF350731

Family and domain databases

Conserved Domains Database

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CDDi
cd00060 FHA, 1 hit
cd15471 Myo5p-like_CBD_afadin, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR037977 CBD_Afadin
IPR002710 Dilute_dom
IPR000253 FHA_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000159 RA_dom
IPR008984 SMAD_FHA_dom_sf
IPR029071 Ubiquitin-like_domsf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01843 DIL, 1 hit
PF00498 FHA, 1 hit
PF00595 PDZ, 1 hit
PF00788 RA, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01132 DIL, 1 hit
SM00240 FHA, 1 hit
SM00228 PDZ, 1 hit
SM00314 RA, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF49879 SSF49879, 1 hit
SSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51126 DILUTE, 1 hit
PS50106 PDZ, 1 hit
PS50200 RA, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (6+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 6 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 4 (identifier: P55196-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK
60 70 80 90 100
AAGNFATKCI RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG
110 120 130 140 150
ERRLDIDEKP LVVQLNWNKD DREGRFVLKN ENDAIPPKKA QSNGPEKQEK
160 170 180 190 200
EGVIQNFKRT LSKKEKKEKK KREKEALRQA SDKDDRPFQG EDVENSRLAA
210 220 230 240 250
EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS DGRPDSGGTL
260 270 280 290 300
RIYADSLKPN IPYKTILLST TDPADFAVAE ALEKYGLEKE NPKDYCIARV
310 320 330 340 350
MLPPGAQHSD EKGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDH
360 370 380 390 400
IPKKTKKHLE GKTPKGKERA DGSGYGSTLP PEKLPYLVEL SPGRRNHFAY
410 420 430 440 450
YNYHTYEDGS DSRDKPKLYR LQLSVTEVGT EKLDDNSIQL FGPGIQPHHC
460 470 480 490 500
DLTNMDGVVT VTPRSMDAET YVEGQRISET TMLQSGMKVQ FGASHVFKFV
510 520 530 540 550
DPSQDHALAK RSVDGGLMVK GPRHKPGIVQ ETTFDLGGDI HSGTALPTSK
560 570 580 590 600
STTRLDSDRV SSASSTAERG MVKPMIRVEQ QPDYRRQESR TQDASGPELI
610 620 630 640 650
LPASIEFRES SEDSFLSAII NYTNSSTVHF KLSPTYVLYM ACRYVLSNQY
660 670 680 690 700
RPDISPTERT HKVIAVVNKM VSMMEGVIQK QKNIAGALAF WMANASELLN
710 720 730 740 750
FIKQDRDLSR ITLDAQDVLA HLVQMAFKYL VHCLQSELNN YMPAFLDDPE
760 770 780 790 800
ENSLQRPKID DVLHTLTGAM SLLRRCRVNA ALTIQLFSQL FHFINMWLFN
810 820 830 840 850
RLVTDPDSGL CSHYWGAIIR QQLGHIEAWA EKQGLELAAD CHLSRIVQAT
860 870 880 890 900
TLLTMDKYAP DDIPNINSTC FKLNSLQLQA LLQNYHCAPD EPFIPTDLIE
910 920 930 940 950
NVVTVAENTA DELARSDGRE VQLEEDPDLQ LPFLLPEDGY SCDVVRNIPN
960 970 980 990 1000
GLQEFLDPLC QRGFCRLIPH TRSPGTWTIY FEGADYESHL LRENTELAQP
1010 1020 1030 1040 1050
LRKEPEIITV TLKKQNGMGL SIVAAKGAGQ DKLGIYVKSV VKGGAADVDG
1060 1070 1080 1090 1100
RLAAGDQLLS VDGRSLVGLS QERAAELMTR TSSVVTLEVA KQGAIYHGLA
1110 1120 1130 1140 1150
TLLNQPSPMM QRISDRRGSG KPRPKSEGFE LYNNSTQNGS PESPQLPWAE
1160 1170 1180 1190 1200
YSEPKKLPGD DRLMKNRADH RSSPNVANQP PSPGGKSAYA SGTTAKITSV
1210 1220 1230 1240 1250
STGNLCTEEQ TPPPRPEAYP IPTQTYTREY FTFPASKSQD RMAPPQNQWP
1260 1270 1280 1290 1300
NYEEKPHMHT DSNHSSIAIQ RVTRSQEELR EDKAYQLERH RIEAAMDRKS
1310 1320 1330 1340 1350
DSDMWINQSS SLDSSTSSQE HLNHSSKSVT PASTLTKSGP GRWKTPAAIP
1360 1370 1380 1390 1400
ATPVAVSQPI RTDLPPPPPP PPVHYAGDFD GMSMDLPLPP PPSANQIGLP
1410 1420 1430 1440 1450
SAQVAAAERR KREEHQRWYE KEKARLEEER ERKRREQERK LGQMRTQSLN
1460 1470 1480 1490 1500
PAPFSPLTAQ QMKPEKPSTL QRPQETVIRE LQPQQQPRTI ERRDLQYITV
1510 1520 1530 1540 1550
SKEELSSGDS LSPDPWKRDA KEKLEKQQQM HIVDMLSKEI QELQSKPDRS
1560 1570 1580 1590 1600
AEESDRLRKL MLEWQFQKRL QESKQKDEDD EEEEDDDVDT MLIMQRLEAE
1610 1620 1630 1640 1650
RRARLQDEER RRQQQLEEMR KREAEDRARQ EEERRRQEEE RTKRDAEEKR
1660 1670 1680 1690 1700
RQEEGYYSRL EAERRRQHDE AARRLLEPEA PGLCRPPLPR DYEPPSPSPA
1710 1720 1730 1740 1750
PGAPPPPPQR NASYLKTQVL SPDSLFTAKF VAYNEEEEEE DCSLAGPNSY
1760 1770 1780 1790 1800
PGSTGAAVGA HDACRDAKEK RSKSQDADSP GSSGAPENLT FKERQRLFSQ
1810 1820
GQDVSNKVKA SRKLTELENE LNTK
Length:1,824
Mass (Da):206,804
Last modified:February 9, 2010 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i51486232F183A3BA
GO
Isoform 1 (identifier: P55196-2) [UniParc]FASTAAdd to basket
Also known as: s-afadin

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     393-407: Missing.
     1605-1628: LQDEERRRQQQLEEMRKREAEDRA → VKGGVLWLCPSVVPILASACFPWG
     1629-1824: Missing.

Note: May be due to intron retention.Curated
Show »
Length:1,612
Mass (Da):182,000
Checksum:i202D7BEC1E825AB6
GO
Isoform 2 (identifier: P55196-1) [UniParc]FASTAAdd to basket
Also known as: l-afadin

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     393-407: Missing.
     1048-1048: Missing.
     1747-1824: PNSYPGSTGA...TELENELNTK → QDKYSSTRKS...KRVTNQLSLS

Note: May be due to intron retention.
Show »
Length:1,816
Mass (Da):205,605
Checksum:iEB1FE7F04879CE8F
GO
Isoform 3 (identifier: P55196-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     393-407: Missing.
     1048-1048: Missing.
     1683-1746: Missing.

Note: No experimental confirmation available.
Show »
Length:1,743
Mass (Da):197,653
Checksum:iECCDE46AD8C401B9
GO
Isoform 6 (identifier: P55196-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1605-1606: Missing.
     1650-1653: RRQE → VMVL
     1654-1824: Missing.

Show »
Length:1,651
Mass (Da):187,672
Checksum:i6F55A481FC0DB6B3
GO
Isoform 5 (identifier: P55196-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-139: Missing.
     1604-1604: R → RTAMPAISVLDL

Note: No experimental confirmation available.
Show »
Length:1,834
Mass (Da):207,788
Checksum:i3D57FDBD6A521DFD
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A8MQ02A8MQ02_HUMAN
Afadin
AFDN
1,781Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3KN01J3KN01_HUMAN
Afadin
AFDN
1,831Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q5TIG5Q5TIG5_HUMAN
Afadin
AFDN
1,665Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0Y7R8H0Y7R8_HUMAN
Afadin
AFDN
389Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9PFY5E9PFY5_HUMAN
Afadin
AFDN
250Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8W9I4F8W9I4_HUMAN
Afadin
AFDN
247Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YA98H0YA98_HUMAN
Afadin
AFDN
167Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0Y8L4H0Y8L4_HUMAN
Afadin
AFDN
313Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0Y8U8H0Y8U8_HUMAN
Afadin
AFDN
124Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0Y948H0Y948_HUMAN
Afadin
AFDN
191Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
There is more potential isoformShow all

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAB82312 differs from that shown. Reason: Frameshift at positions 1509, 1512 and 1701.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti374G → V in AAC50059 (PubMed:8242616).Curated1
Sequence conflicti1425R → P in AAC50059 (PubMed:8242616).Curated1
Isoform 1 (identifier: P55196-2)
Sequence conflicti1031 – 1032DV → D in AAC50059 (PubMed:8242616).Curated2
Sequence conflicti1031 – 1032DV → D in BAA32485 (PubMed:9679199).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_038707139Missing in isoform 1, isoform 2, isoform 3 and isoform 5. 2 Publications1
Alternative sequenceiVSP_038708393 – 407Missing in isoform 1, isoform 2 and isoform 3. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_0387091048Missing in isoform 2 and isoform 3. Curated1
Alternative sequenceiVSP_0192571604R → RTAMPAISVLDL in isoform 5. 1 Publication1
Alternative sequenceiVSP_0002171605 – 1628LQDEE…AEDRA → VKGGVLWLCPSVVPILASAC FPWG in isoform 1. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_0411971605 – 1606Missing in isoform 6. 1 Publication2
Alternative sequenceiVSP_0002181629 – 1824Missing in isoform 1. 1 PublicationAdd BLAST196
Alternative sequenceiVSP_0411981650 – 1653RRQE → VMVL in isoform 6. 1 Publication4
Alternative sequenceiVSP_0411991654 – 1824Missing in isoform 6. 1 PublicationAdd BLAST171
Alternative sequenceiVSP_0387101683 – 1746Missing in isoform 3. CuratedAdd BLAST64
Alternative sequenceiVSP_0387111747 – 1824PNSYP…ELNTK → QDKYSSTRKSHGDLLPAPLK PRPPPCQPRPASDGVFLSNS FQPPSAKANSTAHKKGQPLP PPKKSSSYHPSHCKGRGKRV TNQLSLS in isoform 2. CuratedAdd BLAST78

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U02478 mRNA Translation: AAC50059.1
AB011399 Genomic DNA Translation: BAA32483.1
AB011399 Genomic DNA Translation: BAA32484.1
AB011399 Genomic DNA Translation: BAA32485.1
AL009178 Genomic DNA No translation available.
AL049698 Genomic DNA No translation available.
AL731868 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47482.1
AB209420 mRNA Translation: BAD92657.1
AL161973 mRNA Translation: CAB82312.1 Frameshift.

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS47517.1 [P55196-6]
CCDS75553.1 [P55196-3]

Protein sequence database of the Protein Information Resource

More...
PIRi
T47137

NCBI Reference Sequences

More...
RefSeqi
NP_001035089.1, NM_001040000.2 [P55196-6]
NP_001193937.1, NM_001207008.1 [P55196-3]
NP_001278893.1, NM_001291964.1
XP_005267053.1, XM_005266996.3

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000392108; ENSP00000375956; ENSG00000130396 [P55196-6]
ENST00000392112; ENSP00000375960; ENSG00000130396 [P55196-3]
ENST00000400822; ENSP00000383623; ENSG00000130396 [P55196-5]
ENST00000447894; ENSP00000404595; ENSG00000130396 [P55196-4]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
4301

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:4301

UCSC genome browser

More...
UCSCi
uc003qwc.3 human [P55196-4]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02478 mRNA Translation: AAC50059.1
AB011399 Genomic DNA Translation: BAA32483.1
AB011399 Genomic DNA Translation: BAA32484.1
AB011399 Genomic DNA Translation: BAA32485.1
AL009178 Genomic DNA No translation available.
AL049698 Genomic DNA No translation available.
AL731868 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47482.1
AB209420 mRNA Translation: BAD92657.1
AL161973 mRNA Translation: CAB82312.1 Frameshift.
CCDSiCCDS47517.1 [P55196-6]
CCDS75553.1 [P55196-3]
PIRiT47137
RefSeqiNP_001035089.1, NM_001040000.2 [P55196-6]
NP_001193937.1, NM_001207008.1 [P55196-3]
NP_001278893.1, NM_001291964.1
XP_005267053.1, XM_005266996.3

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T2MNMR-A1003-1094[»]
1XZ9NMR-A1001-1096[»]
2AINNMR-A1003-1094[»]
2EXGNMR-A1001-1096[»]
5A6CX-ray2.90A/B1709-1746[»]
SMRiP55196
ModBaseiSearch...

Protein-protein interaction databases

BioGridi110447, 109 interactors
CORUMiP55196
ELMiP55196
IntActiP55196, 43 interactors
MINTiP55196
STRINGi9606.ENSP00000375960

Chemistry databases

DrugBankiDB08574 (5R)-2-SULFANYL-5-[4-(TRIFLUOROMETHYL)BENZYL]-1,3-THIAZOL-4-ONE

PTM databases

iPTMnetiP55196
PhosphoSitePlusiP55196

Polymorphism and mutation databases

BioMutaiAFDN
DMDMi288558835

Proteomic databases

EPDiP55196
jPOSTiP55196
MassIVEiP55196
PaxDbiP55196
PeptideAtlasiP55196
PRIDEiP55196
ProteomicsDBi56796 [P55196-4]
56797 [P55196-1]
56798 [P55196-2]
56799 [P55196-3]
56800 [P55196-5]
56801 [P55196-6]

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
4301
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000392108; ENSP00000375956; ENSG00000130396 [P55196-6]
ENST00000392112; ENSP00000375960; ENSG00000130396 [P55196-3]
ENST00000400822; ENSP00000383623; ENSG00000130396 [P55196-5]
ENST00000447894; ENSP00000404595; ENSG00000130396 [P55196-4]
GeneIDi4301
KEGGihsa:4301
UCSCiuc003qwc.3 human [P55196-4]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
4301
DisGeNETi4301

GeneCards: human genes, protein and diseases

More...
GeneCardsi
AFDN
HGNCiHGNC:7137 AFDN
HPAiCAB013496
HPA030212
HPA030213
HPA030214
HPA030215
HPA049868
MIMi159559 gene
neXtProtiNX_P55196
OpenTargetsiENSG00000130396

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1892 Eukaryota
ENOG410XR0S LUCA
GeneTreeiENSGT00940000155237
InParanoidiP55196
KOiK05702
OMAiTVYTAKF
OrthoDBi23029at2759
PhylomeDBiP55196
TreeFamiTF350731

Enzyme and pathway databases

ReactomeiR-HSA-418990 Adherens junctions interactions
SignaLinkiP55196
SIGNORiP55196

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
AFDN human
EvolutionaryTraceiP55196

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
MLLT4

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
4301

Pharos

More...
Pharosi
P55196

Protein Ontology

More...
PROi
PR:P55196

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000130396 Expressed in 203 organ(s), highest expression level in oviduct epithelium
ExpressionAtlasiP55196 baseline and differential
GenevisibleiP55196 HS

Family and domain databases

CDDicd00060 FHA, 1 hit
cd15471 Myo5p-like_CBD_afadin, 1 hit
InterProiView protein in InterPro
IPR037977 CBD_Afadin
IPR002710 Dilute_dom
IPR000253 FHA_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000159 RA_dom
IPR008984 SMAD_FHA_dom_sf
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF01843 DIL, 1 hit
PF00498 FHA, 1 hit
PF00595 PDZ, 1 hit
PF00788 RA, 2 hits
SMARTiView protein in SMART
SM01132 DIL, 1 hit
SM00240 FHA, 1 hit
SM00228 PDZ, 1 hit
SM00314 RA, 2 hits
SUPFAMiSSF49879 SSF49879, 1 hit
SSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 2 hits
PROSITEiView protein in PROSITE
PS51126 DILUTE, 1 hit
PS50106 PDZ, 1 hit
PS50200 RA, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAFAD_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P55196
Secondary accession number(s): O75087
, O75088, O75089, Q59FP0, Q5TIG6, Q5TIG7, Q9NSN7, Q9NU92
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 9, 2010
Last modified: September 18, 2019
This is version 205 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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