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Entry version 94 (16 Oct 2019)
Sequence version 1 (23 Apr 2003)
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Protein

Protein 3a

Gene

3a

Organism
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. Up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. Induces apoptosis in cell culture. Downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei133Involved in polymerization1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIon channel, Viral ion channel
Biological processApoptosis, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon receptors by virus, Inhibition of host interferon signaling pathway by virus, Ion transport, Transport, Viral immunoevasion

Protein family/group databases

Transport Classification Database

More...
TCDBi
1.A.57.1.1 the human sars caronavirus viroporin (sars-vp)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Protein 3a
Alternative name(s):
Accessory protein 3a
Protein U274
Protein X1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:3a
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri694009 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaNidoviralesCornidovirineaeCoronaviridaeOrthocoronavirinaeBetacoronavirusSarbecovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiHomo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 34Extracellular1 PublicationAdd BLAST34
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei35 – 55HelicalSequence analysisAdd BLAST21
Topological domaini56 – 78CytoplasmicSequence analysisAdd BLAST23
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Topological domaini100 – 104ExtracellularSequence analysis5
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Topological domaini126 – 274Cytoplasmic1 PublicationAdd BLAST149

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host cytoplasm, Host Golgi apparatus, Host membrane, Membrane, Secreted, Virion

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi27S → G: Complete loss of O-glycosylation; when associated with A-28; A-32 and A-34. 1 Publication1
Mutagenesisi28T → A: Complete loss of O-glycosylation; when associated with A-27; A-32 and A-34. 1 Publication1
Mutagenesisi32T → A: Complete loss of O-glycosylation; when associated with A-27; A-28 and A-34. 1 Publication1
Mutagenesisi34T → A: Complete loss of O-glycosylation; when associated with A-27; A-28 and A-32. 1 Publication1
Mutagenesisi81C → A: No effect on polymerization. 1 Publication1
Mutagenesisi117C → A: No effect on polymerization. 1 Publication1
Mutagenesisi121C → A: No effect on polymerization. 1 Publication1
Mutagenesisi127C → A: No effect on polymerization. 1 Publication1
Mutagenesisi130C → A: No effect on polymerization. 1 Publication1
Mutagenesisi133C → A: Almost complete loss of polimerization ability. 1 Publication1
Mutagenesisi148C → A: No effect on polymerization. 1 Publication1
Mutagenesisi157C → A: No effect on polymerization. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001061311 – 274Protein 3aAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi27O-linked (GalNAc...) serine; by hostSequence analysis1
Glycosylationi28O-linked (GalNAc...) threonine; by host1 Publication1
Glycosylationi32O-linked (GalNAc...) threonine; by host1 Publication1
Glycosylationi34O-linked (GalNAc...) threonine; by hostSequence analysis1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Exists in both O-glycosylated and non-glycosylated forms. The glycosylated form is associated with the virion.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P59632

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P59632

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotetramer composed of two homodimers linked non covalently.

Interacts with M, S and E proteins.

Also interacts with the accessory protein 7a.

3 Publications

Protein-protein interaction databases

Database of interacting proteins

More...
DIPi
DIP-61251N

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P59632

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi83 – 86Poly-Leu4
Compositional biasi267 – 270Poly-Thr4

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The second or the third transmembrane region are responsible for Golgi localization.

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR024407 SARS_coronavirus_Orf3/3a

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF11289 APA3_viroporin, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P59632-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDLFMRFFTL GSITAQPVKI DNASPASTVH ATATIPLQAS LPFGWLVIGV
60 70 80 90 100
AFLAVFQSAT KIIALNKRWQ LALYKGFQFI CNLLLLFVTI YSHLLLVAAG
110 120 130 140 150
MEAQFLYLYA LIYFLQCINA CRIIMRCWLC WKCKSKNPLL YDANYFVCWH
160 170 180 190 200
THNYDYCIPY NSVTDTIVVT EGDGISTPKL KEDYQIGGYS EDRHSGVKDY
210 220 230 240 250
VVVHGYFTEV YYQLESTQIT TDTGIENATF FIFNKLVKDP PNVQIHTIDG
260 270
SSGVANPAMD PIYDEPTTTT SVPL
Length:274
Mass (Da):30,903
Last modified:April 23, 2003 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE48C7D44A4DE34FE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti11G → R in strain: Isolate Tor2, Isolate BJ02 and Isolate BJ03. 1
Natural varianti20I → T in strain: Isolate Shanghai LY. 1
Natural varianti29V → A in strain: Isolate Shanghai QXC1. 1
Natural varianti101M → K in strain: Isolate HKU-39849. 1
Natural varianti129L → F in strain: Isolate TWK. 1
Natural varianti136K → Q in strain: Isolate BJ01. 1
Natural varianti171E → A in strain: Isolate GD01. 1
Natural varianti193R → W in strain: Isolate GD01. 1
Natural varianti222D → N in strain: Isolate Shanghai QXC1. 1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY278741 Genomic RNA Translation: AAP13446.1
AY274119 Genomic RNA Translation: AAP41038.1
AY282752 Genomic RNA No translation available.
AY278554 Genomic RNA Translation: AAP13568.1
AY278491 Genomic RNA No translation available.
AY278487 Genomic RNA No translation available.
AY278488 Genomic RNA Translation: AAP30031.1
AY278489 Genomic RNA Translation: AAP51228.1
AY278490 Genomic RNA No translation available.
AY279354 Genomic RNA No translation available.
AY291451 Genomic RNA Translation: AAP37018.1
AY310120 Genomic RNA Translation: AAP50486.1
AY291315 Genomic RNA Translation: AAP33698.1
AY338174 Genomic RNA Translation: AAQ01598.1
AY338175 Genomic RNA Translation: AAQ01610.1
AY348314 Genomic RNA Translation: AAP97883.1
AP006557 Genomic RNA Translation: BAC81349.1
AP006558 Genomic RNA Translation: BAC81363.1
AP006559 Genomic RNA Translation: BAC81377.1
AP006560 Genomic RNA Translation: BAC81391.1
AP006561 Genomic RNA Translation: BAC81405.1
AY323977 Genomic RNA Translation: AAP72975.1
AY427439 Genomic RNA Translation: AAQ94061.1
AY463059 Genomic RNA Translation: AAP82984.2
AY322207 Genomic RNA Translation: AAP82969.1

NCBI Reference Sequences

More...
RefSeqi
NP_828852.2, NC_004718.3

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1489669

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vg:1489669

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY278741 Genomic RNA Translation: AAP13446.1
AY274119 Genomic RNA Translation: AAP41038.1
AY282752 Genomic RNA No translation available.
AY278554 Genomic RNA Translation: AAP13568.1
AY278491 Genomic RNA No translation available.
AY278487 Genomic RNA No translation available.
AY278488 Genomic RNA Translation: AAP30031.1
AY278489 Genomic RNA Translation: AAP51228.1
AY278490 Genomic RNA No translation available.
AY279354 Genomic RNA No translation available.
AY291451 Genomic RNA Translation: AAP37018.1
AY310120 Genomic RNA Translation: AAP50486.1
AY291315 Genomic RNA Translation: AAP33698.1
AY338174 Genomic RNA Translation: AAQ01598.1
AY338175 Genomic RNA Translation: AAQ01610.1
AY348314 Genomic RNA Translation: AAP97883.1
AP006557 Genomic RNA Translation: BAC81349.1
AP006558 Genomic RNA Translation: BAC81363.1
AP006559 Genomic RNA Translation: BAC81377.1
AP006560 Genomic RNA Translation: BAC81391.1
AP006561 Genomic RNA Translation: BAC81405.1
AY323977 Genomic RNA Translation: AAP72975.1
AY427439 Genomic RNA Translation: AAQ94061.1
AY463059 Genomic RNA Translation: AAP82984.2
AY322207 Genomic RNA Translation: AAP82969.1
RefSeqiNP_828852.2, NC_004718.3

3D structure databases

SMRiP59632
ModBaseiSearch...

Protein-protein interaction databases

DIPiDIP-61251N

Protein family/group databases

TCDBi1.A.57.1.1 the human sars caronavirus viroporin (sars-vp)

PTM databases

iPTMnetiP59632

Proteomic databases

PRIDEiP59632

Genome annotation databases

GeneIDi1489669
KEGGivg:1489669

Family and domain databases

InterProiView protein in InterPro
IPR024407 SARS_coronavirus_Orf3/3a
PfamiView protein in Pfam
PF11289 APA3_viroporin, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAP3A_CVHSA
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P59632
Secondary accession number(s): Q7T6R6
, Q7TA10, Q7TA18, Q7TFB0, Q7TLD0, Q80BV5
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: October 16, 2019
This is version 94 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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