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Entry version 166 (31 Jul 2019)
Sequence version 1 (01 Nov 1997)
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Protein

FACT complex subunit POB3

Gene

POB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.9 Publications

Miscellaneous

In contrast to the orthologous protein in animals and plants, this protein does not contain a HMG box DNA-binding domain. This function may instead be provided by the HMG box of the associated NHP6A/NHP6B proteins in the FACT complex of yeast.
Present with 22400 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processDNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-32664-MONOMER

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-75955 RNA Polymerase II Transcription Elongation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
FACT complex subunit POB3
Alternative name(s):
Facilitates chromatin transcription complex subunit POB3
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:POB3
Ordered Locus Names:YML069W
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XIII

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YML069W

Saccharomyces Genome Database

More...
SGDi
S000004534 POB3

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi20R → H in pob3-11; causes severe defects in rate of growth; when associated with C-109. 1 Publication1
Mutagenesisi78L → R in pob3-1; causes severe defects in rate of growth; when associated with K-419 and T-489. 1 Publication1
Mutagenesisi109R → C in pob3-11; causes severe defects in rate of growth; when associated with H-20. 1 Publication1
Mutagenesisi308Q → A or D: No effect. 1 Publication1
Mutagenesisi308Q → K: Confers sensitivity to HU indicating a disturbed activity in DNA replication; confers a SPT- phenotype indicating a disturbed activity in transcription. 1 Publication1
Mutagenesisi308Q → R: Confers sensitivity to HU indicating a disturbed activity in DNA replication. 1 Publication1
Mutagenesisi311T → A: No change of sensitivity to HU; confers a SPT- phenotype indicating a disturbed activity in transcription. 1 Publication1
Mutagenesisi419M → K in pob3-1; causes severe defects in rate of growth; when associated with R-78 and T-489. 1 Publication1
Mutagenesisi489S → T in pob3-1; causes severe defects in rate of growth; when associated with R-78 and K-419. 1 Publication1
Mutagenesisi547K → M in pob3-21; causes severe defects in rate of growth. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002032501 – 552FACT complex subunit POB3Add BLAST552

Proteomic databases

MaxQB - The MaxQuant DataBase

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MaxQBi
Q04636

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q04636

PRoteomics IDEntifications database

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PRIDEi
Q04636

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q04636

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Forms a stable heterodimer with SPT16. The SPT16-POB3 dimer weakly associates with multiple molecules of NHP6 (NHP6A or NHP6B) to form the FACT (yFACT or SNP) complex. The FACT complex interacts with the CK2 (casein kinase II) complex subunits CKA1, CKA2, CKB1 and CKB2 and the components of the transcription machinery CHD1, CTR9, PAF1 and CDC73. The FACT complex interacts with the PAF1 complex. SPT16 interacts with SAS3 and POL1.

Interacts directly with RFA1.

9 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
35097, 702 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-3215 FACT complex

Database of interacting proteins

More...
DIPi
DIP-4083N

Protein interaction database and analysis system

More...
IntActi
Q04636, 49 interactors

Molecular INTeraction database

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MINTi
Q04636

STRING: functional protein association networks

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STRINGi
4932.YML069W

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1552
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q04636

Database of comparative protein structure models

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ModBasei
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
Q04636

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the SSRP1 family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000180790

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q04636

KEGG Orthology (KO)

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KOi
K09272

Identification of Orthologs from Complete Genome Data

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OMAi
PRGRYDV

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
2.30.29.220, 1 hit
2.30.29.30, 2 hits

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR013719 DUF1747
IPR011993 PH-like_dom_sf
IPR035417 POB3_N
IPR000969 SSrcognition
IPR024954 SSRP1_dom
IPR038167 SSRP1_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF17292 POB3_N, 1 hit
PF08512 Rtt106, 1 hit
PF03531 SSrecog, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR00887 SSRCOGNITION

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01287 Rtt106, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q04636-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTDFDRIYL NQSKFSGRFR IADSGLGWKI STSGGSAANQ ARKPFLLPAT
60 70 80 90 100
ELSTVQWSRG CRGYDLKINT KNQGVIQLDG FSQDDYNLIK NDFHRRFNIQ
110 120 130 140 150
VEQREHSLRG WNWGKTDLAR NEMVFALNGK PTFEIPYARI NNTNLTSKNE
160 170 180 190 200
VGIEFNIQDE EYQPAGDELV EMRFYIPGVI QTNVDENMTK KEESSNEVVP
210 220 230 240 250
KKEDGAEGED VQMAVEEKSM AEAFYEELKE KADIGEVAGD AIVSFQDVFF
260 270 280 290 300
TTPRGRYDID IYKNSIRLRG KTYEYKLQHR QIQRIVSLPK ADDIHHLLVL
310 320 330 340 350
AIEPPLRQGQ TTYPFLVLQF QKDEETEVQL NLEDEDYEEN YKDKLKKQYD
360 370 380 390 400
AKTHIVLSHV LKGLTDRRVI VPGEYKSKYD QCAVSCSFKA NEGYLYPLDN
410 420 430 440 450
AFFFLTKPTL YIPFSDVSMV NISRAGQTST SSRTFDLEVV LRSNRGSTTF
460 470 480 490 500
ANISKEEQQL LEQFLKSKNL RVKNEDREVQ ERLQTALGSD SDEEDINMGS
510 520 530 540 550
AGEDDESVDE DFQVSSDNDA DEVAEEFDSD AALSDAEGGS DEERPSKKPK

VE
Length:552
Mass (Da):62,994
Last modified:November 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i02C04F620C7B1450
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z38114 Genomic DNA Translation: CAA86251.1
BK006946 Genomic DNA Translation: DAA09828.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S48328

NCBI Reference Sequences

More...
RefSeqi
NP_013642.1, NM_001182428.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YML069W_mRNA; YML069W_mRNA; YML069W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
854933

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YML069W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38114 Genomic DNA Translation: CAA86251.1
BK006946 Genomic DNA Translation: DAA09828.1
PIRiS48328
RefSeqiNP_013642.1, NM_001182428.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GCJX-ray2.55A/B/C/D220-478[»]
2GCLX-ray2.21A/B220-478[»]
3F5RX-ray1.70A1-168[»]
4PQ0X-ray1.65A232-473[»]
SMRiQ04636
ModBaseiSearch...

Protein-protein interaction databases

BioGridi35097, 702 interactors
ComplexPortaliCPX-3215 FACT complex
DIPiDIP-4083N
IntActiQ04636, 49 interactors
MINTiQ04636
STRINGi4932.YML069W

PTM databases

iPTMnetiQ04636

Proteomic databases

MaxQBiQ04636
PaxDbiQ04636
PRIDEiQ04636

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML069W_mRNA; YML069W_mRNA; YML069W
GeneIDi854933
KEGGisce:YML069W

Organism-specific databases

EuPathDBiFungiDB:YML069W
SGDiS000004534 POB3

Phylogenomic databases

HOGENOMiHOG000180790
InParanoidiQ04636
KOiK09272
OMAiPRGRYDV

Enzyme and pathway databases

BioCyciYEAST:G3O-32664-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

EvolutionaryTraceiQ04636

Protein Ontology

More...
PROi
PR:Q04636

Family and domain databases

Gene3Di2.30.29.220, 1 hit
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR013719 DUF1747
IPR011993 PH-like_dom_sf
IPR035417 POB3_N
IPR000969 SSrcognition
IPR024954 SSRP1_dom
IPR038167 SSRP1_sf
PfamiView protein in Pfam
PF17292 POB3_N, 1 hit
PF08512 Rtt106, 1 hit
PF03531 SSrecog, 1 hit
PRINTSiPR00887 SSRCOGNITION
SMARTiView protein in SMART
SM01287 Rtt106, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPOB3_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q04636
Secondary accession number(s): D6VZA4
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 31, 2019
This is version 166 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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