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Entry version 178 (08 May 2019)
Sequence version 3 (05 Oct 2010)
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Protein

Separin

Gene

ESPL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage. EC:3.4.22.49

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent. PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage. Second, phosphorylation at Ser-1126 inactivates it. The complete phosphorylation during mitosis, is removed when cells undergo anaphase. Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin and inhibitory phosphate.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei20291

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processChromosome partition

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

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BioCyci
MetaCyc:ENSG00000135476-MONOMER

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-2467813 Separation of Sister Chromatids

SIGNOR Signaling Network Open Resource

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SIGNORi
Q14674

Protein family/group databases

MEROPS protease database

More...
MEROPSi
C50.002

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Separin (EC:3.4.22.49)
Alternative name(s):
Caspase-like protein ESPL1
Extra spindle poles-like 1 protein
Separase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ESPL1
Synonyms:ESP1, KIAA0165
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 12

Organism-specific databases

Human Gene Nomenclature Database

More...
HGNCi
HGNC:16856 ESPL1

Online Mendelian Inheritance in Man (OMIM)

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MIMi
604143 gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q14674

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi1126S → A: Abolishes phosphorylation at this site, as well as the negative regulation due to phosphorylation. 1
Mutagenesisi1483 – 1486EIMR → RIME: Abolishes autocleavage; when associated with R-1178; E-1181; R-1207 and E-1210. Does not affect the protease function. 1 Publication4
Mutagenesisi1486R → A: Abolishes autocleavage; when associated with A-1181 and A-1210. 1 Publication1
Mutagenesisi1503 – 1506EILR → RILE: Does not affect autocleavage. Does not affect the protease function. 1 Publication4
Mutagenesisi1506R → A: Abolishes autocleavage; when associated with A-1161 and A-1210. 1 Publication1
Mutagenesisi1532 – 1535ELLR → RLLE: Strongly reduces autocleavage at this site, but enhances autocleavage at site 1. Does not affect the protease function. 1 Publication4
Mutagenesisi1535R → A: Abolishes autocleavage; when associated with A-1161 and A-1281. 1 Publication1
Mutagenesisi2029C → A: Abolishes protease activity. 1 Publication1

Organism-specific databases

DisGeNET

More...
DisGeNETi
9700

Open Targets

More...
OpenTargetsi
ENSG00000135476

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA27884

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
ESPL1

Domain mapping of disease mutations (DMDM)

More...
DMDMi
308153600

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002059001 – 2120SeparinAdd BLAST2120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1126Phosphoserine1 Publication1
Modified residuei1396PhosphoserineCombined sources1
Modified residuei1399PhosphoserineCombined sources1
Modified residuei1508PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei1506 – 1507Cleavage; by autolysis2
Sitei1535 – 1536Cleavage; by autolysis2

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
Q14674

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q14674

MaxQB - The MaxQuant DataBase

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MaxQBi
Q14674

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q14674

PeptideAtlas

More...
PeptideAtlasi
Q14674

PRoteomics IDEntifications database

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PRIDEi
Q14674

ProteomicsDB human proteome resource

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ProteomicsDBi
60103
60104 [Q14674-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q14674

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q14674

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000135476 Expressed in 131 organ(s), highest expression level in secondary oocyte

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q14674 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q14674 HS

Organism-specific databases

Human Protein Atlas

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HPAi
CAB012185

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with PTTG1.

Interacts with RAD21.

2 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
115052, 53 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q14674

Database of interacting proteins

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DIPi
DIP-46079N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

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ELMi
Q14674

Protein interaction database and analysis system

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IntActi
Q14674, 24 interactors

Molecular INTeraction database

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MINTi
Q14674

STRING: functional protein association networks

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STRINGi
9606.ENSP00000257934

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1945 – 2040Peptidase C50Add BLAST96

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1849 Eukaryota
COG5155 LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00390000004990

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000290657

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q14674

KEGG Orthology (KO)

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KOi
K02365

Identification of Orthologs from Complete Genome Data

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OMAi
LWLYICT

Database of Orthologous Groups

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OrthoDBi
14013at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q14674

TreeFam database of animal gene trees

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TreeFami
TF101169

Family and domain databases

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR005314 Peptidase_C50
IPR030397 SEPARIN_core_dom

The PANTHER Classification System

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PANTHERi
PTHR12792 PTHR12792, 1 hit

PROSITE; a protein domain and family database

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PROSITEi
View protein in PROSITE
PS51700 SEPARIN, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q14674-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRSFKRVNFG TLLSSQKEAE ELLPALKEFL SNPPAGFPSS RSDAERRQAC
60 70 80 90 100
DAILRACNQQ LTAKLACPRH LGSLLELAEL ACDGYLVSTP QRPPLYLERI
110 120 130 140 150
LFVLLRNAAA QGSPEATLRL AQPLHACLVQ CSREAAPQDY EAVARGSFSL
160 170 180 190 200
LWKGAEALLE RRAAFAARLK ALSFLVLLED ESTPCEVPHF ASPTACRAVA
210 220 230 240 250
AHQLFDASGH GLNEADADFL DDLLSRHVIR ALVGERGSSS GLLSPQRALC
260 270 280 290 300
LLELTLEHCR RFCWSRHHDK AISAVEKAHS YLRNTNLAPS LQLCQLGVKL
310 320 330 340 350
LQVGEEGPQA VAKLLIKASA VLSKSMEAPS PPLRALYESC QFFLSGLERG
360 370 380 390 400
TKRRYRLDAI LSLFAFLGGY CSLLQQLRDD GVYGGSSKQQ QSFLQMYFQG
410 420 430 440 450
LHLYTVVVYD FAQGCQIVDL ADLTQLVDSC KSTVVWMLEA LEGLSGQELT
460 470 480 490 500
DHMGMTASYT SNLAYSFYSH KLYAEACAIS EPLCQHLGLV KPGTYPEVPP
510 520 530 540 550
EKLHRCFRLQ VESLKKLGKQ AQGCKMVILW LAALQPCSPE HMAEPVTFWV
560 570 580 590 600
RVKMDAARAG DKELQLKTLR DSLSGWDPET LALLLREELQ AYKAVRADTG
610 620 630 640 650
QERFNIICDL LELSPEETPA GAWARATHLV ELAQVLCYHD FTQQTNCSAL
660 670 680 690 700
DAIREALQLL DSVRPEAQAR DQLLDDKAQA LLWLYICTLE AKMQEGIERD
710 720 730 740 750
RRAQAPGNLE EFEVNDLNYE DKLQEDRFLY SNIAFNLAAD AAQSKCLDQA
760 770 780 790 800
LALWKELLTK GQAPAVRCLQ QTAASLQILA ALYQLVAKPM QALEVLLLLR
810 820 830 840 850
IVSERLKDHS KAAGSSCHIT QLLLTLGCPS YAQLHLEEAA SSLKHLDQTT
860 870 880 890 900
DTYLLLSLTC DLLRSQLYWT HQKVTKGVSL LLSVLRDPAL QKSSKAWYLL
910 920 930 940 950
RVQVLQLVAA YLSLPSNNLS HSLWEQLCAQ GWQTPEIALI DSHKLLRSII
960 970 980 990 1000
LLLMGSDILS TQKAAVETSF LDYGENLVQK WQVLSEVLSC SEKLVCHLGR
1010 1020 1030 1040 1050
LGSVSEAKAF CLEALKLTTK LQIPRQCALF LVLKGELELA RNDIDLCQSD
1060 1070 1080 1090 1100
LQQVLFLLES CTEFGGVTQH LDSVKKVHLQ KGKQQAQVPC PPQLPEEELF
1110 1120 1130 1140 1150
LRGPALELVA TVAKEPGPIA PSTNSSPVLK TKPQPIPNFL SHSPTCDCSL
1160 1170 1180 1190 1200
CASPVLTAVC LRWVLVTAGV RLAMGHQAQG LDLLQVVLKG CPEAAERLTQ
1210 1220 1230 1240 1250
ALQASLNHKT PPSLVPSLLD EILAQAYTLL ALEGLNQPSN ESLQKVLQSG
1260 1270 1280 1290 1300
LKFVAARIPH LEPWRASLLL IWALTKLGGL SCCTTQLFAS SWGWQPPLIK
1310 1320 1330 1340 1350
SVPGSEPSKT QGQKRSGRGR QKLASAPLRL NNTSQKGLEG RGLPCTPKPP
1360 1370 1380 1390 1400
DRIRQAGPHV PFTVFEEVCP TESKPEVPQA PRVQQRVQTR LKVNFSDDSD
1410 1420 1430 1440 1450
LEDPVSAEAW LAEEPKRRGT ASRGRGRARK GLSLKTDAVV APGSAPGNPG
1460 1470 1480 1490 1500
LNGRSRRAKK VASRHCEERR PQRASDQARP GPEIMRTIPE EELTDNWRKM
1510 1520 1530 1540 1550
SFEILRGSDG EDSASGGKTP APGPEAASGE WELLRLDSSK KKLPSPCPDK
1560 1570 1580 1590 1600
ESDKDLGPRL RLPSAPVATG LSTLDSICDS LSVAFRGISH CPPSGLYAHL
1610 1620 1630 1640 1650
CRFLALCLGH RDPYATAFLV TESVSITCRH QLLTHLHRQL SKAQKHRGSL
1660 1670 1680 1690 1700
EIADQLQGLS LQEMPGDVPL ARIQRLFSFR ALESGHFPQP EKESFQERLA
1710 1720 1730 1740 1750
LIPSGVTVCV LALATLQPGT VGNTLLLTRL EKDSPPVSVQ IPTGQNKLHL
1760 1770 1780 1790 1800
RSVLNEFDAI QKAQKENSSC TDKREWWTGR LALDHRMEVL IASLEKSVLG
1810 1820 1830 1840 1850
CWKGLLLPSS EEPGPAQEAS RLQELLQDCG WKYPDRTLLK IMLSGAGALT
1860 1870 1880 1890 1900
PQDIQALAYG LCPTQPERAQ ELLNEAVGRL QGLTVPSNSH LVLVLDKDLQ
1910 1920 1930 1940 1950
KLPWESMPSL QALPVTRLPS FRFLLSYSII KEYGASPVLS QGVDPRSTFY
1960 1970 1980 1990 2000
VLNPHNNLSS TEEQFRANFS SEAGWRGVVG EVPRPEQVQE ALTKHDLYIY
2010 2020 2030 2040 2050
AGHGAGARFL DGQAVLRLSC RAVALLFGCS SAALAVRGNL EGAGIVLKYI
2060 2070 2080 2090 2100
MAGCPLFLGN LWDVTDRDID RYTEALLQGW LGAGPGAPLL YYVNQARQAP
2110 2120
RLKYLIGAAP IAYGLPVSLR
Length:2,120
Mass (Da):233,175
Last modified:October 5, 2010 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0257A69093B4954C
GO
Isoform 2 (identifier: Q14674-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-325: Missing.

Note: No experimental confirmation available.
Show »
Length:1,795
Mass (Da):197,745
Checksum:i47FC0C2740C5A400
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BRX7H3BRX7_HUMAN
Separin
ESPL1
207Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H3BM31H3BM31_HUMAN
Separin
ESPL1
49Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti25A → D in AAR18247 (PubMed:12194817).Curated1
Sequence conflicti116A → V in AAR18247 (PubMed:12194817).Curated1
Sequence conflicti693M → I in AAR18247 (PubMed:12194817).Curated1
Sequence conflicti693M → I in BAA11482 (PubMed:8724849).Curated1
Sequence conflicti1329R → S in AAR18247 (PubMed:12194817).Curated1
Sequence conflicti1329R → S in BAA11482 (PubMed:8724849).Curated1
Sequence conflicti1561R → Q in AAR18247 (PubMed:12194817).Curated1
Sequence conflicti1561R → Q in BAA11482 (PubMed:8724849).Curated1
Sequence conflicti2037R → H in AAR18247 (PubMed:12194817).Curated1
Sequence conflicti2037R → H in BAA11482 (PubMed:8724849).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_057703614S → R. Corresponds to variant dbSNP:rs1318648Ensembl.1
Natural variantiVAR_057704699R → Q. Corresponds to variant dbSNP:rs34424268Ensembl.1
Natural variantiVAR_0577051136I → V. Corresponds to variant dbSNP:rs34130634Ensembl.1
Natural variantiVAR_0577061157T → A. Corresponds to variant dbSNP:rs35428211Ensembl.1
Natural variantiVAR_0577071237Q → H. Corresponds to variant dbSNP:rs34396464Ensembl.1
Natural variantiVAR_0577081435K → M. Corresponds to variant dbSNP:rs1110719Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0093611 – 325Missing in isoform 2. 1 PublicationAdd BLAST325

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AY455930 mRNA Translation: AAR18247.1
D79987 mRNA Translation: BAA11482.2
AC021072 Genomic DNA No translation available.
AC073611 Genomic DNA No translation available.

The Consensus CDS (CCDS) project

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CCDSi
CCDS8852.1 [Q14674-1]

NCBI Reference Sequences

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RefSeqi
NP_036423.4, NM_012291.4 [Q14674-1]
XP_006719768.1, XM_006719705.3 [Q14674-1]
XP_011537326.1, XM_011539024.2 [Q14674-1]
XP_016875742.1, XM_017020253.1 [Q14674-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENST00000257934; ENSP00000257934; ENSG00000135476 [Q14674-1]
ENST00000552462; ENSP00000449831; ENSG00000135476 [Q14674-1]

Database of genes from NCBI RefSeq genomes

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GeneIDi
9700

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:9700

UCSC genome browser

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UCSCi
uc001sck.2 human [Q14674-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY455930 mRNA Translation: AAR18247.1
D79987 mRNA Translation: BAA11482.2
AC021072 Genomic DNA No translation available.
AC073611 Genomic DNA No translation available.
CCDSiCCDS8852.1 [Q14674-1]
RefSeqiNP_036423.4, NM_012291.4 [Q14674-1]
XP_006719768.1, XM_006719705.3 [Q14674-1]
XP_011537326.1, XM_011539024.2 [Q14674-1]
XP_016875742.1, XM_017020253.1 [Q14674-2]

3D structure databases

Database of comparative protein structure models

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ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGridi115052, 53 interactors
CORUMiQ14674
DIPiDIP-46079N
ELMiQ14674
IntActiQ14674, 24 interactors
MINTiQ14674
STRINGi9606.ENSP00000257934

Protein family/group databases

MEROPSiC50.002

PTM databases

iPTMnetiQ14674
PhosphoSitePlusiQ14674

Polymorphism and mutation databases

BioMutaiESPL1
DMDMi308153600

Proteomic databases

EPDiQ14674
jPOSTiQ14674
MaxQBiQ14674
PaxDbiQ14674
PeptideAtlasiQ14674
PRIDEiQ14674
ProteomicsDBi60103
60104 [Q14674-2]

Protocols and materials databases

The DNASU plasmid repository

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DNASUi
9700
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257934; ENSP00000257934; ENSG00000135476 [Q14674-1]
ENST00000552462; ENSP00000449831; ENSG00000135476 [Q14674-1]
GeneIDi9700
KEGGihsa:9700
UCSCiuc001sck.2 human [Q14674-1]

Organism-specific databases

Comparative Toxicogenomics Database

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CTDi
9700
DisGeNETi9700

GeneCards: human genes, protein and diseases

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GeneCardsi
ESPL1

H-Invitational Database, human transcriptome db

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H-InvDBi
HIX0201902
HGNCiHGNC:16856 ESPL1
HPAiCAB012185
MIMi604143 gene
neXtProtiNX_Q14674
OpenTargetsiENSG00000135476
PharmGKBiPA27884

Human Unidentified Gene-Encoded large proteins database

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HUGEi
Search...

GenAtlas: human gene database

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GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1849 Eukaryota
COG5155 LUCA
GeneTreeiENSGT00390000004990
HOGENOMiHOG000290657
InParanoidiQ14674
KOiK02365
OMAiLWLYICT
OrthoDBi14013at2759
PhylomeDBiQ14674
TreeFamiTF101169

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000135476-MONOMER
ReactomeiR-HSA-2467813 Separation of Sister Chromatids
SIGNORiQ14674

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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ChiTaRSi
ESPL1 human

The Gene Wiki collection of pages on human genes and proteins

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GeneWikii
Separase

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
9700

Protein Ontology

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PROi
PR:Q14674

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Gene expression databases

BgeeiENSG00000135476 Expressed in 131 organ(s), highest expression level in secondary oocyte
ExpressionAtlasiQ14674 baseline and differential
GenevisibleiQ14674 HS

Family and domain databases

InterProiView protein in InterPro
IPR005314 Peptidase_C50
IPR030397 SEPARIN_core_dom
PANTHERiPTHR12792 PTHR12792, 1 hit
PROSITEiView protein in PROSITE
PS51700 SEPARIN, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiESPL1_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q14674
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 5, 2010
Last modified: May 8, 2019
This is version 178 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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