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Entry version 191 (03 Jul 2019)
Sequence version 4 (11 Sep 2007)
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Protein

Mediator of RNA polymerase II transcription subunit 1

Gene

MED1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).12 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-1368082 RORA activates gene expression
R-HSA-1368108 BMAL1:CLOCK,NPAS2 activates circadian gene expression
R-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-2151201 Transcriptional activation of mitochondrial biogenesis
R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
R-HSA-383280 Nuclear Receptor transcription pathway
R-HSA-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
R-HSA-400253 Circadian Clock
R-HSA-9018519 Estrogen-dependent gene expression

SignaLink: a signaling pathway resource with multi-layered regulatory networks

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SignaLinki
Q15648

SIGNOR Signaling Network Open Resource

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SIGNORi
Q15648

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 1
Alternative name(s):
Activator-recruited cofactor 205 kDa component
Short name:
ARC205
Mediator complex subunit 1
Peroxisome proliferator-activated receptor-binding protein
Short name:
PBP
Short name:
PPAR-binding protein
Thyroid hormone receptor-associated protein complex 220 kDa component
Short name:
Trap220
Thyroid receptor-interacting protein 2
Short name:
TR-interacting protein 2
Short name:
TRIP-2
Vitamin D receptor-interacting protein complex component DRIP205
p53 regulatory protein RB18A
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MED1
Synonyms:ARC205, CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:9234 MED1

Online Mendelian Inheritance in Man (OMIM)

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MIMi
604311 gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_Q15648

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi599 – 612SQNPI…LQITG → EKHKILHRLLQDSS: Enhances interaction with ESR1. 1 PublicationAdd BLAST14
Mutagenesisi600 – 612QNPIL…LQITG → RHKILHRLLQEGS: Enhances interaction with ESR1. 1 PublicationAdd BLAST13
Mutagenesisi604L → A: Impairs interaction with ESR2; when associated with A-607; A-645 and A-648. 1 Publication1
Mutagenesisi607 – 608LL → AA: Impairs interaction with ESR1, PPARG, RXRA and THRB. Impairs interaction with THRA; when associated with 648-A-A-649. 6 Publications2
Mutagenesisi607L → A: Impairs interaction with ESR2; when associated with A-604; A-645 and A-648. 1 Publication1
Mutagenesisi639 – 653TKNHP…LKDNP → VSRHKILHRLLQEGS: Enhances interaction with ESR1. 1 PublicationAdd BLAST15
Mutagenesisi645L → A: Impairs interaction with ESR2; when associated with A-604; A-607 and A-648. 1 Publication1
Mutagenesisi648 – 649LL → AA: Impairs interaction with ESR1, PPARG, THRB and VDR. Impairs interaction with THRA; when associated with 607-A-A-608. 6 Publications2
Mutagenesisi648L → A: Impairs interaction with ESR2; when associated with A-604; A-607 and A-645. 1 Publication1
Mutagenesisi1032T → A: Enhances protein stability; when associated with A-1457. 1 Publication1
Mutagenesisi1457T → A: Enhances protein stability; when associated with A-1032. 1 Publication1

Organism-specific databases

DisGeNET

More...
DisGeNETi
5469

Open Targets

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OpenTargetsi
ENSG00000125686

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA33556

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
MED1

Domain mapping of disease mutations (DMDM)

More...
DMDMi
158518535

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000585521 – 1581Mediator of RNA polymerase II transcription subunit 1Add BLAST1581

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei588PhosphoserineCombined sources1
Modified residuei664PhosphoserineCombined sources1
Modified residuei795PhosphoserineCombined sources1
Modified residuei805PhosphothreonineCombined sources1
Modified residuei953PhosphoserineBy similarity1
Modified residuei1032Phosphothreonine; by MAPK1 or MAPK31 Publication1
Modified residuei1051PhosphothreonineCombined sources1
Modified residuei1057PhosphothreonineCombined sources1
Modified residuei1156PhosphoserineCombined sources1
Modified residuei1177N6-acetyllysineCombined sources1
Modified residuei1207PhosphoserineCombined sources1
Modified residuei1215PhosphothreonineCombined sources1
Modified residuei1223PhosphoserineCombined sources1
Modified residuei1302PhosphoserineCombined sources1
Modified residuei1347PhosphoserineCombined sources1
Modified residuei1403PhosphoserineCombined sources1
Modified residuei1433PhosphoserineCombined sources1
Modified residuei1440PhosphothreonineCombined sources1
Modified residuei1457Phosphothreonine; by MAPK1 or MAPK31 Publication1
Modified residuei1463PhosphoserineCombined sources1
Modified residuei1465PhosphoserineBy similarity1
Modified residuei1479PhosphoserineCombined sources1
Modified residuei1481PhosphoserineCombined sources1
Modified residuei1482PhosphoserineCombined sources1
Modified residuei1529N6-acetyllysineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
Q15648

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q15648

MaxQB - The MaxQuant DataBase

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MaxQBi
Q15648

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q15648

PeptideAtlas

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PeptideAtlasi
Q15648

PRoteomics IDEntifications database

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PRIDEi
Q15648

ProteomicsDB human proteome resource

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ProteomicsDBi
60685
60686 [Q15648-3]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q15648

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q15648

SwissPalm database of S-palmitoylation events

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SwissPalmi
Q15648

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000125686 Expressed in 236 organ(s), highest expression level in upper lobe of lung

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q15648 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q15648 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
CAB017696
HPA052818

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. This subunit specifically interacts with a number of nuclear receptors in a ligand-dependent fashion including AR, ESR1, ESR2, PPARA, PPARG, RORA, RXRA, RXRG, THRA, THRB and VDR.

Interacts with CTNNB1, GABPA, GLI3, PPARGC1A and TP53.

Interacts with YWHAH.

Interacts with CLOCK; this interaction requires the presence of THRAP3 (By similarity).

Interacts with GATA1 and CCAR1.

Interacts with NR4A3 (By similarity).

By similarity23 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
111465, 125 interactors

ComplexPortal: manually curated resource of macromolecular complexes

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ComplexPortali
CPX-3227 Core mediator complex

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q15648

Database of interacting proteins

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DIPi
DIP-24212N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

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ELMi
Q15648

Protein interaction database and analysis system

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IntActi
Q15648, 53 interactors

Molecular INTeraction database

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MINTi
Q15648

STRING: functional protein association networks

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STRINGi
9606.ENSP00000300651

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11581
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q15648

Database of comparative protein structure models

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ModBasei
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
Q15648

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 670Interaction with the Mediator complex and THRAAdd BLAST670
Regioni16 – 590Interaction with ESR1Add BLAST575
Regioni108 – 212Interaction with the Mediator complexAdd BLAST105
Regioni215 – 390Interaction with the Mediator complexAdd BLAST176
Regioni405 – 644Interaction with THRAAdd BLAST240
Regioni542 – 789Interaction with VDRAdd BLAST248
Regioni622 – 701Interaction with PPARGC1A and THRA1 PublicationAdd BLAST80
Regioni656 – 1066Interaction with ESR1Add BLAST411
Regioni681 – 715Interaction with GATA11 PublicationAdd BLAST35
Regioni1249 – 1421Interaction with TP531 PublicationAdd BLAST173

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi604 – 608LXXLL motif 15
Motifi645 – 649LXXLL motif 25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1078 – 1482Ser-richAdd BLAST405
Compositional biasi1496 – 1529Lys-richAdd BLAST34

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Mediator complex subunit 1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
ENOG410IEFR Eukaryota
ENOG410XR2E LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00660000095569

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q15648

KEGG Orthology (KO)

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KOi
K15144

Identification of Orthologs from Complete Genome Data

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OMAi
HPVDNKG

Database of Orthologous Groups

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OrthoDBi
57581at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q15648

TreeFam database of animal gene trees

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TreeFami
TF324954

Family and domain databases

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR019680 Mediator_Med1

Pfam protein domain database

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Pfami
View protein in Pfam
PF10744 Med1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q15648-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKAQGETEES EKLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM
60 70 80 90 100
SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNGL GSHLSASGTE
110 120 130 140 150
CYITSDMFYV EVQLDPAGQL CDVKVAHHGE NPVSCPELVQ QLREKNFDEF
160 170 180 190 200
SKHLKGLVNL YNLPGDNKLK TKMYLALQSL EQDLSKMAIM YWKATNAGPL
210 220 230 240 250
DKILHGSVGY LTPRSGGHLM NLKYYVSPSD LLDDKTASPI ILHENNVSRS
260 270 280 290 300
LGMNASVTIE GTSAVYKLPI APLIMGSHPV DNKWTPSFSS ITSANSVDLP
310 320 330 340 350
ACFFLKFPQP IPVSRAFVQK LQNCTGIPLF ETQPTYAPLY ELITQFELSK
360 370 380 390 400
DPDPIPLNHN MRFYAALPGQ QHCYFLNKDA PLPDGRSLQG TLVSKITFQH
410 420 430 440 450
PGRVPLILNL IRHQVAYNTL IGSCVKRTIL KEDSPGLLQF EVCPLSESRF
460 470 480 490 500
SVSFQHPVND SLVCVVMDVQ DSTHVSCKLY KGLSDALICT DDFIAKVVQR
510 520 530 540 550
CMSIPVTMRA IRRKAETIQA DTPALSLIAE TVEDMVKKNL PPASSPGYGM
560 570 580 590 600
TTGNNPMSGT TTPTNTFPGG PITTLFNMSM SIKDRHESVG HGEDFSKVSQ
610 620 630 640 650
NPILTSLLQI TGNGGSTIGS SPTPPHHTPP PVSSMAGNTK NHPMLMNLLK
660 670 680 690 700
DNPAQDFSTL YGSSPLERQN SSSGSPRMEI CSGSNKTKKK KSSRLPPEKP
710 720 730 740 750
KHQTEDDFQR ELFSMDVDSQ NPIFDVNMTA DTLDTPHITP APSQCSTPPT
760 770 780 790 800
TYPQPVPHPQ PSIQRMVRLS SSDSIGPDVT DILSDIAEEA SKLPSTSDDC
810 820 830 840 850
PAIGTPLRDS SSSGHSQSTL FDSDVFQTNN NENPYTDPAD LIADAAGSPS
860 870 880 890 900
SDSPTNHFFH DGVDFNPDLL NSQSQSGFGE EYFDESSQSG DNDDFKGFAS
910 920 930 940 950
QALNTLGVPM LGGDNGETKF KGNNQADTVD FSIISVAGKA LAPADLMEHH
960 970 980 990 1000
SGSQGPLLTT GDLGKEKTQK RVKEGNGTSN STLSGPGLDS KPGKRSRTPS
1010 1020 1030 1040 1050
NDGKSKDKPP KRKKADTEGK SPSHSSSNRP FTPPTSTGGS KSPGSAGRSQ
1060 1070 1080 1090 1100
TPPGVATPPI PKITIQIPKG TVMVGKPSSH SQYTSSGSVS SSGSKSHHSH
1110 1120 1130 1140 1150
SSSSSSSAST SGKMKSSKSE GSSSSKLSSS MYSSQGSSGS SQSKNSSQSG
1160 1170 1180 1190 1200
GKPGSSPITK HGLSSGSSST KMKPQGKPSS LMNPSLSKPN ISPSHSRPPG
1210 1220 1230 1240 1250
GSDKLASPMK PVPGTPPSSK AKSPISSGSG GSHMSGTSSS SGMKSSSGLG
1260 1270 1280 1290 1300
SSGSLSQKTP PSSNSCTASS SSFSSSGSSM SSSQNQHGSS KGKSPSRNKK
1310 1320 1330 1340 1350
PSLTAVIDKL KHGVVTSGPG GEDPLDGQMG VSTNSSSHPM SSKHNMSGGE
1360 1370 1380 1390 1400
FQGKREKSDK DKSKVSTSGS SVDSSKKTSE SKNVGSTGVA KIIISKHDGG
1410 1420 1430 1440 1450
SPSIKAKVTL QKPGESSGEG LRPQMASSKN YGSPLISGST PKHERGSPSH
1460 1470 1480 1490 1500
SKSPAYTPQN LDSESESGSS IAEKSYQNSP SSDDGIRPLP EYSTEKHKKH
1510 1520 1530 1540 1550
KKEKKKVKDK DRDRDRDKDR DKKKSHSIKP ESWSKSPISS DQSLSMTSNT
1560 1570 1580
ILSADRPSRL SPDFMIGEED DDLMDVALIG N
Length:1,581
Mass (Da):168,478
Last modified:September 11, 2007 - v4
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iFCE0FE87EF08B887
GO
Isoform 2 (identifier: Q15648-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     548-556: YGMTTGNNP → SKNPELGSG
     557-1581: Missing.

Show »
Length:556
Mass (Da):61,563
Checksum:i1E8BBE45A2629DB1
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KSD7J3KSD7_HUMAN
Mediator of RNA polymerase II trans...
MED1
79Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QKZ7J3QKZ7_HUMAN
Mediator of RNA polymerase II trans...
MED1
97Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAC39854 differs from that shown. Reason: Frameshift at positions 543 and 545.Curated
The sequence AAH06517 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence CAA73867 differs from that shown. Reason: Frameshift at position 4.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti86R → G in CAA73867 (PubMed:9444950).Curated1
Sequence conflicti147F → S in CAA73867 (PubMed:9444950).Curated1
Sequence conflicti471 – 472DS → GL in CAA73867 (PubMed:9444950).Curated2
Sequence conflicti563P → S in CAA73867 (PubMed:9444950).Curated1
Sequence conflicti563P → S in AAF98352 (PubMed:10733574).Curated1
Sequence conflicti573T → A in CAA73867 (PubMed:9444950).Curated1
Sequence conflicti573T → A in AAF98352 (PubMed:10733574).Curated1
Sequence conflicti651D → N in AAH06517 (PubMed:15489334).Curated1
Sequence conflicti673S → F in AAC41736 (PubMed:7776974).Curated1
Sequence conflicti702 – 708Missing in AAC41736 (PubMed:7776974).Curated7
Sequence conflicti721N → K in AAC39854 (PubMed:9653119).Curated1
Sequence conflicti728M → R in AAF98352 (PubMed:10733574).Curated1
Sequence conflicti756 – 761VPHPQP → FYLTPQ in AAH06517 (PubMed:15489334).Curated6
Sequence conflicti1388G → S in AAC39854 (PubMed:9653119).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_053955753P → T. Corresponds to variant dbSNP:rs1139825Ensembl.1
Natural variantiVAR_0349381240S → G. Corresponds to variant dbSNP:rs35668211Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_027906548 – 556YGMTTGNNP → SKNPELGSG in isoform 2. 1 Publication9
Alternative sequenceiVSP_027907557 – 1581Missing in isoform 2. 1 PublicationAdd BLAST1025

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Y13467 mRNA Translation: CAA73867.1 Frameshift.
AF055994 mRNA Translation: AAC39854.1 Frameshift.
CH471152 Genomic DNA Translation: EAW60575.1
BC006517 mRNA Translation: AAH06517.1 Different termination.
BC060758 mRNA Translation: AAH60758.1
BC131783 mRNA Translation: AAI31784.1
AF283812 mRNA Translation: AAF98352.1
L40366 mRNA Translation: AAC41736.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS11336.1 [Q15648-1]

NCBI Reference Sequences

More...
RefSeqi
NP_004765.2, NM_004774.3 [Q15648-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000300651; ENSP00000300651; ENSG00000125686 [Q15648-1]
ENST00000394287; ENSP00000377828; ENSG00000125686 [Q15648-3]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
5469

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:5469

UCSC genome browser

More...
UCSCi
uc002hru.3 human [Q15648-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13467 mRNA Translation: CAA73867.1 Frameshift.
AF055994 mRNA Translation: AAC39854.1 Frameshift.
CH471152 Genomic DNA Translation: EAW60575.1
BC006517 mRNA Translation: AAH06517.1 Different termination.
BC060758 mRNA Translation: AAH60758.1
BC131783 mRNA Translation: AAI31784.1
AF283812 mRNA Translation: AAF98352.1
L40366 mRNA Translation: AAC41736.1
CCDSiCCDS11336.1 [Q15648-1]
RefSeqiNP_004765.2, NM_004774.3 [Q15648-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RJKX-ray1.99C640-652[»]
1RK3X-ray2.20C640-652[»]
1RKGX-ray1.90C640-652[»]
1RKHX-ray2.28C640-652[»]
2O4JX-ray1.74C640-652[»]
2O4RX-ray1.98C640-652[»]
2ZFXX-ray1.99C640-652[»]
3A2HX-ray2.50B640-652[»]
3AUNX-ray1.81B640-652[»]
3VJSX-ray1.93C640-652[»]
3VJTX-ray2.00C640-652[»]
3VRTX-ray2.40C640-652[»]
3VRUX-ray2.00C640-652[»]
3VRVX-ray1.90C640-652[»]
3VRWX-ray2.40C640-652[»]
3W0GX-ray1.94C640-652[»]
3W0HX-ray1.80C640-652[»]
3W0IX-ray1.90C640-652[»]
3W0JX-ray1.84C640-652[»]
3W5PX-ray1.90C640-652[»]
3W5QX-ray1.90C640-652[»]
3W5RX-ray2.20C640-652[»]
3W5TX-ray2.29C640-652[»]
3WT5X-ray1.90C640-652[»]
3WT6X-ray2.00C640-652[»]
3WT7X-ray2.40C640-652[»]
3WTQX-ray2.10C640-652[»]
4YNKX-ray2.30C640-652[»]
5AWJX-ray2.20C640-652[»]
5AWKX-ray2.90C640-652[»]
5B41X-ray1.89C640-652[»]
5B5BX-ray2.00C/F640-652[»]
5GICX-ray2.35C641-650[»]
5GIDX-ray2.15C641-649[»]
5GIEX-ray2.39C/E641-650[»]
5XPMX-ray2.20C640-652[»]
5XPNX-ray1.96C640-652[»]
5XPOX-ray2.28C640-652[»]
5XPPX-ray2.85C640-652[»]
5XUQX-ray2.80C640-652[»]
5XZFX-ray2.10C640-652[»]
5XZHX-ray2.00C640-652[»]
5ZWEX-ray2.72C640-652[»]
5ZWFX-ray2.10C640-652[»]
5ZWHX-ray2.38C640-652[»]
5ZWIX-ray2.40C640-652[»]
6D94X-ray1.90B632-655[»]
SMRiQ15648
ModBaseiSearch...

Protein-protein interaction databases

BioGridi111465, 125 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ15648
DIPiDIP-24212N
ELMiQ15648
IntActiQ15648, 53 interactors
MINTiQ15648
STRINGi9606.ENSP00000300651

PTM databases

iPTMnetiQ15648
PhosphoSitePlusiQ15648
SwissPalmiQ15648

Polymorphism and mutation databases

BioMutaiMED1
DMDMi158518535

Proteomic databases

EPDiQ15648
jPOSTiQ15648
MaxQBiQ15648
PaxDbiQ15648
PeptideAtlasiQ15648
PRIDEiQ15648
ProteomicsDBi60685
60686 [Q15648-3]

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
5469
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300651; ENSP00000300651; ENSG00000125686 [Q15648-1]
ENST00000394287; ENSP00000377828; ENSG00000125686 [Q15648-3]
GeneIDi5469
KEGGihsa:5469
UCSCiuc002hru.3 human [Q15648-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
5469
DisGeNETi5469

GeneCards: human genes, protein and diseases

More...
GeneCardsi
MED1
HGNCiHGNC:9234 MED1
HPAiCAB017696
HPA052818
MIMi604311 gene
neXtProtiNX_Q15648
OpenTargetsiENSG00000125686
PharmGKBiPA33556

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiENOG410IEFR Eukaryota
ENOG410XR2E LUCA
GeneTreeiENSGT00660000095569
InParanoidiQ15648
KOiK15144
OMAiHPVDNKG
OrthoDBi57581at2759
PhylomeDBiQ15648
TreeFamiTF324954

Enzyme and pathway databases

ReactomeiR-HSA-1368082 RORA activates gene expression
R-HSA-1368108 BMAL1:CLOCK,NPAS2 activates circadian gene expression
R-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-2151201 Transcriptional activation of mitochondrial biogenesis
R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
R-HSA-383280 Nuclear Receptor transcription pathway
R-HSA-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
R-HSA-400253 Circadian Clock
R-HSA-9018519 Estrogen-dependent gene expression
SignaLinkiQ15648
SIGNORiQ15648

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
MED1 human
EvolutionaryTraceiQ15648

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
MED1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
5469

Protein Ontology

More...
PROi
PR:Q15648

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000125686 Expressed in 236 organ(s), highest expression level in upper lobe of lung
ExpressionAtlasiQ15648 baseline and differential
GenevisibleiQ15648 HS

Family and domain databases

InterProiView protein in InterPro
IPR019680 Mediator_Med1
PfamiView protein in Pfam
PF10744 Med1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMED1_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q15648
Secondary accession number(s): A2RRQ6
, O43810, O75447, Q6P9H7, Q6PK58, Q9HD39
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 11, 2007
Last modified: July 3, 2019
This is version 191 of the entry and version 4 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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