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Entry version 144 (18 Sep 2019)
Sequence version 3 (05 Apr 2011)
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Protein

Rho guanine nucleotide exchange factor 28

Gene

ARHGEF28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri652 – 699Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST48

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGuanine-nucleotide releasing factor, RNA-binding
Biological processDifferentiation
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-3928662 EPHB-mediated forward signaling

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 28
Alternative name(s):
190 kDa guanine nucleotide exchange factor
Short name:
p190-RhoGEF
Short name:
p190RhoGEF
Rho guanine nucleotide exchange factor
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ARHGEF28
Synonyms:KIAA1998, RGNEF
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

Organism-specific databases

Human Gene Nomenclature Database

More...
HGNCi
HGNC:30322 ARHGEF28

Online Mendelian Inheritance in Man (OMIM)

More...
MIMi
612790 gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q8N1W1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

DisGeNET

More...
DisGeNETi
64283

Open Targets

More...
OpenTargetsi
ENSG00000214944

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
ARHGEF28

Domain mapping of disease mutations (DMDM)

More...
DMDMi
327478563

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003241191 – 1705Rho guanine nucleotide exchange factor 28Add BLAST1705

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei313PhosphoserineBy similarity1
Modified residuei478PhosphoserineBy similarity1
Modified residuei624PhosphoserineCombined sources1
Modified residuei1538PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated on tyrosine upon stimulation of cells by laminin.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q8N1W1

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
Q8N1W1

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...
MassIVEi
Q8N1W1

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q8N1W1

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q8N1W1

PeptideAtlas

More...
PeptideAtlasi
Q8N1W1

PRoteomics IDEntifications database

More...
PRIDEi
Q8N1W1

ProteomicsDB human proteome resource

More...
ProteomicsDBi
19386
6165
71639 [Q8N1W1-1]
71640 [Q8N1W1-2]
71641 [Q8N1W1-3]
71642 [Q8N1W1-4]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q8N1W1

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q8N1W1

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSG00000214944 Expressed in 193 organ(s), highest expression level in stomach

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q8N1W1 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q8N1W1 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
HPA037602

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homooligomer; forms cytoplasmic aggregates.

Forms a complex with MAPK8 and MAPK8IP1.

Interacts with RHOA.

Interacts with microtubules.

Interacts with YWHAE and YWHAH.

Interacts with PTK2/FAK1.

Interacts with NEFL (By similarity).

Interacts with CTNND2; prevents interaction with RHOA.

By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
122127, 7 interactors

Protein interaction database and analysis system

More...
IntActi
Q8N1W1, 5 interactors

Molecular INTeraction database

More...
MINTi
Q8N1W1

STRING: functional protein association networks

More...
STRINGi
9606.ENSP00000441913

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11705
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q8N1W1

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini849 – 1044DHPROSITE-ProRule annotationAdd BLAST196
Domaini1086 – 1188PHPROSITE-ProRule annotationAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1295 – 1304Interaction with PTK2/FAK1; required for regulation of axonal branching and synapse formationBy similarity10
Regioni1372 – 1383Mediates cytoplasmic retention and interaction with YWHAHBy similarityAdd BLAST12
Regioni1425 – 1705Interaction with microtubulesBy similarityAdd BLAST281
Regioni1496 – 1527RNA-bindingBy similarityAdd BLAST32
Regioni1566 – 1579Mediates cytoplasmic retention and interaction with MAPK8IP1By similarityAdd BLAST14

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili1488 – 1525Sequence analysisAdd BLAST38

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri652 – 699Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3520 Eukaryota
KOG4305 Eukaryota
ENOG410XT68 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000155831

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000154109

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q8N1W1

KEGG Orthology (KO)

More...
KOi
K21072

Identification of Orthologs from Complete Genome Data

More...
OMAi
EIYANCM

Database of Orthologous Groups

More...
OrthoDBi
69816at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q8N1W1

TreeFam database of animal gene trees

More...
TreeFami
TF334740

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00029 C1, 1 hit
cd14680 PH_p190RhoGEF, 1 hit
cd00160 RhoGEF, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.900.10, 1 hit
2.30.29.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR037819 ARHGEF28_PH
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR041020 PH_16
IPR001849 PH_domain

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00130 C1_1, 1 hit
PF17838 PH_16, 1 hit
PF00621 RhoGEF, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00109 C1, 1 hit
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48065 SSF48065, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (6+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 6 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8N1W1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELSCSEAPL YGQMMIYAKF DKNVYLPEDA EFYFTYDGSH QRHVMIAERI
60 70 80 90 100
EDNVLQSSVP GHGLQETVTV SVCLCSEGYS PVTMGSGSVT YVDNMACRLA
110 120 130 140 150
RLLVTQANRL TACSHQTLLT PFALTAGALP ALDEELVLAL THLELPLEWT
160 170 180 190 200
VLGSSSLEVS SHRESLLHLA MRWGLAKLSQ FFLCLPGGVQ ALALPNEEGA
210 220 230 240 250
TPLDLALREG HSKLVEDVTN FQGRWSPSFS RVQLSEEASL HYIHSSETLT
260 270 280 290 300
LTLNHTAEHL LEADIKLFRK YFWDRAFLVK AFEPEARPEE RTAMPSSGAE
310 320 330 340 350
TEEEIKNSVS SRSAAEKEDI KRVKSLVVQH NEHEDQHSLD LDRSFDILKK
360 370 380 390 400
SKPPSTLLAA GRLSDMLNGG DEVYANCMVI DQVGDLDISY INIEGITATT
410 420 430 440 450
SPESRGCTLW PQSSKHTLPT ETSPSVYPLS ENVEGTAHTE AQQSFMSPSS
460 470 480 490 500
SCASNLNLSF GWHGFEKEQS HLKKRSSSLD ALDADSEGEG HSEPSHICYT
510 520 530 540 550
PGSQSSSRTG IPSGDELDSF ETNTEPDFNI SRAESLPLSS NLQSKESLLS
560 570 580 590 600
GVRSRSYSCS SPKISLGKTR LVRELTVCSS SEEQRAYSLS EPPRENRIQE
610 620 630 640 650
EEWDKYIIPA KSESEKYKVS RTFSFLMNRM TSPRNKSKTK SKDAKDKEKL
660 670 680 690 700
NRHQFAPGTF SGVLQCLVCD KTLLGKESLQ CSNCNANVHK GCKDAAPACT
710 720 730 740 750
KKFQEKYNKN KPQTILGNSS FRDIPQPGLS LHPSSSVPVG LPTGRRETVG
760 770 780 790 800
QVHPLSRSVP GTTLESFRRS ATSLESESDH NSCRSRSHSD ELLQSMGSSP
810 820 830 840 850
STESFIMEDV VDSSLWSDLS SDAQEFEAES WSLVVDPSFC NRQEKDVIKR
860 870 880 890 900
QDVIFELMQT EMHHIQTLFI MSEIFRKGMK EELQLDHSTV DKIFPCLDEL
910 920 930 940 950
LEIHRHFFYS MKERRQESCA GSDRNFVIDR IGDILVQQFS EENASKMKKI
960 970 980 990 1000
YGEFCCHHKE AVNLFKELQQ NKKFQNFIKL RNSNLLARRR GIPECILLVT
1010 1020 1030 1040 1050
QRITKYPVLV ERILQYTKER TEEHKDLRKA LCLIKDMIAT VDLKVNEYEK
1060 1070 1080 1090 1100
NQKWLEILNK IENKTYTKLK NGHVFRKQAL MSEERTLLYD GLVYWKTATG
1110 1120 1130 1140 1150
RFKDILALLL TDVLLFLQEK DQKYIFAAVD QKPSVISLQK LIAREVANEE
1160 1170 1180 1190 1200
RGMFLISASS AGPEMYEIHT NSKEERNNWM RRIQQAVESC PEEKGGRTSE
1210 1220 1230 1240 1250
SDEDKRKAEA RVAKIQQCQE ILTNQDQQIC AYLEEKLHIY AELGELSGFE
1260 1270 1280 1290 1300
DVHLEPHLLI KPDPGEPPQA ASLLAAALKE AESLQVAVKA SQMGAVSQSC
1310 1320 1330 1340 1350
EDSCGDSVLA DTLSSHDVPG SPTASLVTGG REGRGCSDVD PGIQGVVTDL
1360 1370 1380 1390 1400
AVSDAGEKVE CRNFPGSSQS EIIQAIQNLT RLLYSLQAAL TIQDSHIEIH
1410 1420 1430 1440 1450
RLVLQQQEGL SLGHSILRGG PLQDQKSRDA DRQHEELANV HQLQHQLQQE
1460 1470 1480 1490 1500
QRRWLRRCEQ QQRAQATRES WLQERERECQ SQEELLLRSR GELDLQLQEY
1510 1520 1530 1540 1550
QHSLERLREG QRLVEREQAR MRAQQSLLGH WKHGRQRSLP AVLLPGGPEV
1560 1570 1580 1590 1600
MELNRSESLC HENSFFINEA LVQMSFNTFN KLNPSVIHQD ATYPTTQSHS
1610 1620 1630 1640 1650
DLVRTSEHQV DLKVDPSQPS NVSHKLWTAA GSGHQILPFH ESSKDSCKND
1660 1670 1680 1690 1700
LDTSHTESPT PHDSNSHRPQ LQAFITEAKL NLPTRTMTRQ DGETGDGAKE

NIVYL
Length:1,705
Mass (Da):191,891
Last modified:April 5, 2011 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD4DD6331BD259CC7
GO
Isoform 2 (identifier: Q8N1W1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     583-602: EQRAYSLSEPPRENRIQEEE → GKHHLTFGFENTLFQNRFFS
     603-1705: Missing.

Note: No experimental confirmation available.
Show »
Length:602
Mass (Da):66,373
Checksum:i7A7A0474CC0A9D43
GO
Isoform 3 (identifier: Q8N1W1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1282-1325: Missing.
     1649-1649: N → NGSSMTKCSCTLTSPPGLWTGTTSTLK

Note: No experimental confirmation available.
Show »
Length:1,687
Mass (Da):190,184
Checksum:iB1CD6ACC1DE291B1
GO
Isoform 4 (identifier: Q8N1W1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1650-1705: DLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL → GN

Show »
Length:1,651
Mass (Da):185,844
Checksum:i880C23E7F41826D4
GO
Isoform 5 (identifier: Q8N1W1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MELSCSEAPLYGQMMIYAKFDKNVYLPE → MDSDSDSPFNYSWPSFPKMKIRRRTSKQ
     29-341: Missing.

Note: No experimental confirmation available.
Show »
Length:1,392
Mass (Da):157,251
Checksum:i0D9EB6EF948012B8
GO
Isoform 6 (identifier: Q8N1W1-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1649-1649: N → NGSSMTKCSCTLTSPPGLWTGTTSTLK

Note: Gene prediction based on EST data.
Show »
Length:1,731
Mass (Da):194,519
Checksum:i302A50B721ABB567
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RGZ3D6RGZ3_HUMAN
Rho guanine nucleotide exchange fac...
ARHGEF28
651Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
D6RAP0D6RAP0_HUMAN
Rho guanine nucleotide exchange fac...
ARHGEF28
83Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH12946 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB15243 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC02707 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti1450E → G in BAB15141 (PubMed:14702039).Curated1
Sequence conflicti1540P → S in BAB15141 (PubMed:14702039).Curated1
Sequence conflicti1598S → F in AAH12946 (PubMed:15489334).Curated1
Sequence conflicti1613K → L in AAH12946 (PubMed:15489334).Curated1
Sequence conflicti1620S → L in AAH12946 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_03965798R → M. Corresponds to variant dbSNP:rs12659447Ensembl.1
Natural variantiVAR_039658225W → R. Corresponds to variant dbSNP:rs7714670EnsemblClinVar.1
Natural variantiVAR_039659284P → Q1 PublicationCorresponds to variant dbSNP:rs6453022EnsemblClinVar.1
Natural variantiVAR_039660544S → L. Corresponds to variant dbSNP:rs2973571EnsemblClinVar.1
Natural variantiVAR_039661585R → K. Corresponds to variant dbSNP:rs2973566EnsemblClinVar.1
Natural variantiVAR_039662780H → N. Corresponds to variant dbSNP:rs2973558EnsemblClinVar.1
Natural variantiVAR_0396631548P → S. Corresponds to variant dbSNP:rs17634865EnsemblClinVar.1
Natural variantiVAR_0396641640H → Q2 PublicationsCorresponds to variant dbSNP:rs1478453EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0447371 – 28MELSC…VYLPE → MDSDSDSPFNYSWPSFPKMK IRRRTSKQ in isoform 5. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_04473829 – 341Missing in isoform 5. 1 PublicationAdd BLAST313
Alternative sequenceiVSP_032140583 – 602EQRAY…IQEEE → GKHHLTFGFENTLFQNRFFS in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_032141603 – 1705Missing in isoform 2. 1 PublicationAdd BLAST1103
Alternative sequenceiVSP_0321421282 – 1325Missing in isoform 3. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_0321431649N → NGSSMTKCSCTLTSPPGLWT GTTSTLK in isoform 3 and isoform 6. 2 Publications1
Alternative sequenceiVSP_0321441650 – 1705DLDTS…NIVYL → GN in isoform 4. CuratedAdd BLAST56

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB082529 mRNA Translation: BAC02707.1 Different initiation.
AK025470 mRNA Translation: BAB15141.1
AK025816 mRNA Translation: BAB15243.1 Different initiation.
AK094713 mRNA Translation: BAC04405.1
AK304761 mRNA Translation: BAG65516.1
AC008387 Genomic DNA No translation available.
AC026702 Genomic DNA No translation available.
AC091868 Genomic DNA No translation available.
AC093283 Genomic DNA No translation available.
BC012946 mRNA Translation: AAH12946.1 Different initiation.
BC157846 mRNA Translation: AAI57847.1
BC171850 mRNA Translation: AAI71850.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS47231.2 [Q8N1W1-6]
CCDS54870.1 [Q8N1W1-1]
CCDS58957.1 [Q8N1W1-5]

NCBI Reference Sequences

More...
RefSeqi
NP_001073948.2, NM_001080479.2 [Q8N1W1-6]
NP_001171164.1, NM_001177693.1 [Q8N1W1-1]
NP_001231293.1, NM_001244364.1 [Q8N1W1-5]
XP_011541848.1, XM_011543546.1 [Q8N1W1-6]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000296794; ENSP00000296794; ENSG00000214944 [Q8N1W1-4]
ENST00000296799; ENSP00000296799; ENSG00000214944 [Q8N1W1-5]
ENST00000426542; ENSP00000412175; ENSG00000214944 [Q8N1W1-1]
ENST00000437974; ENSP00000411459; ENSG00000214944 [Q8N1W1-6]
ENST00000513042; ENSP00000441436; ENSG00000214944 [Q8N1W1-1]
ENST00000545377; ENSP00000441913; ENSG00000214944 [Q8N1W1-6]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
64283

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:64283

UCSC genome browser

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UCSCi
uc010izf.4 human [Q8N1W1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB082529 mRNA Translation: BAC02707.1 Different initiation.
AK025470 mRNA Translation: BAB15141.1
AK025816 mRNA Translation: BAB15243.1 Different initiation.
AK094713 mRNA Translation: BAC04405.1
AK304761 mRNA Translation: BAG65516.1
AC008387 Genomic DNA No translation available.
AC026702 Genomic DNA No translation available.
AC091868 Genomic DNA No translation available.
AC093283 Genomic DNA No translation available.
BC012946 mRNA Translation: AAH12946.1 Different initiation.
BC157846 mRNA Translation: AAI57847.1
BC171850 mRNA Translation: AAI71850.1
CCDSiCCDS47231.2 [Q8N1W1-6]
CCDS54870.1 [Q8N1W1-1]
CCDS58957.1 [Q8N1W1-5]
RefSeqiNP_001073948.2, NM_001080479.2 [Q8N1W1-6]
NP_001171164.1, NM_001177693.1 [Q8N1W1-1]
NP_001231293.1, NM_001244364.1 [Q8N1W1-5]
XP_011541848.1, XM_011543546.1 [Q8N1W1-6]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6BC0X-ray2.20A1049-1194[»]
6BC1X-ray2.90C/D1049-1194[»]
SMRiQ8N1W1
ModBaseiSearch...

Protein-protein interaction databases

BioGridi122127, 7 interactors
IntActiQ8N1W1, 5 interactors
MINTiQ8N1W1
STRINGi9606.ENSP00000441913

PTM databases

iPTMnetiQ8N1W1
PhosphoSitePlusiQ8N1W1

Polymorphism and mutation databases

BioMutaiARHGEF28
DMDMi327478563

Proteomic databases

EPDiQ8N1W1
jPOSTiQ8N1W1
MassIVEiQ8N1W1
MaxQBiQ8N1W1
PaxDbiQ8N1W1
PeptideAtlasiQ8N1W1
PRIDEiQ8N1W1
ProteomicsDBi19386
6165
71639 [Q8N1W1-1]
71640 [Q8N1W1-2]
71641 [Q8N1W1-3]
71642 [Q8N1W1-4]

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
64283
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296794; ENSP00000296794; ENSG00000214944 [Q8N1W1-4]
ENST00000296799; ENSP00000296799; ENSG00000214944 [Q8N1W1-5]
ENST00000426542; ENSP00000412175; ENSG00000214944 [Q8N1W1-1]
ENST00000437974; ENSP00000411459; ENSG00000214944 [Q8N1W1-6]
ENST00000513042; ENSP00000441436; ENSG00000214944 [Q8N1W1-1]
ENST00000545377; ENSP00000441913; ENSG00000214944 [Q8N1W1-6]
GeneIDi64283
KEGGihsa:64283
UCSCiuc010izf.4 human [Q8N1W1-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
64283
DisGeNETi64283

GeneCards: human genes, protein and diseases

More...
GeneCardsi
ARHGEF28
HGNCiHGNC:30322 ARHGEF28
HPAiHPA037602
MIMi612790 gene
neXtProtiNX_Q8N1W1
OpenTargetsiENSG00000214944

Human Unidentified Gene-Encoded large proteins database

More...
HUGEi
Search...

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG3520 Eukaryota
KOG4305 Eukaryota
ENOG410XT68 LUCA
GeneTreeiENSGT00940000155831
HOGENOMiHOG000154109
InParanoidiQ8N1W1
KOiK21072
OMAiEIYANCM
OrthoDBi69816at2759
PhylomeDBiQ8N1W1
TreeFamiTF334740

Enzyme and pathway databases

ReactomeiR-HSA-3928662 EPHB-mediated forward signaling

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
ARHGEF28 human

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
64283

Pharos

More...
Pharosi
Q8N1W1

Protein Ontology

More...
PROi
PR:Q8N1W1

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000214944 Expressed in 193 organ(s), highest expression level in stomach
ExpressionAtlasiQ8N1W1 baseline and differential
GenevisibleiQ8N1W1 HS

Family and domain databases

CDDicd00029 C1, 1 hit
cd14680 PH_p190RhoGEF, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037819 ARHGEF28_PH
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR041020 PH_16
IPR001849 PH_domain
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF17838 PH_16, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiARG28_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8N1W1
Secondary accession number(s): B2RXG7
, B4E3K4, B5MDA3, B7ZW32, E9PC75, Q8NCM7, Q96E37, Q9H6L3, Q9H6W0
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: April 5, 2011
Last modified: September 18, 2019
This is version 144 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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