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Entry version 74 (31 Jul 2019)
Sequence version 1 (01 Mar 2002)
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Protein

Dihydrofolate reductase

Gene

dfr2d

Organism
Escherichia coli
Status
Unreviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein predictedi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (D4587_00460), Dihydrofolate reductase type 8 (dhfrVIII), Dihydrofolate reductase type 9 (dhfrIX), Dihydrofolate reductase (BTQ06_28630), Dihydrofolate reductase, Dihydrofolate reductase (APZ14_00990), Dihydrofolate reductase (dfrB2), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase type A10 (dfrA10), Dihydrofolate reductase (dfrA21), Dihydrofolate reductase (C9Y95_01915), Dihydrofolate reductase (folA), Dihydrofolate reductase (C9224_00785), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase (D9F05_04510), Dihydrofolate reductase (C5715_27395), Dihydrofolate reductase (DUM94_12960), Dihydrofolate reductase type 2, Dihydrofolate reductase (dfrB3), Dihydrofolate reductase type 2, Dihydrofolate reductase type 1 (dhfrI), Dihydrofolate reductase (DS143_29305), Dihydrofolate reductase (C9003_10310), Dihydrofolate reductase type 5 (dhfrV), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (DXF93_00550), Dihydrofolate reductase type 15 (dhfrXV), Dihydrofolate reductase (dfrII), Dihydrofolate reductase (D9J03_00795), Dihydrofolate reductase (EBA78_10940), Dihydrofolate reductase (D7Z87_07315), Dihydrofolate reductase (BHS87_26495), Dihydrofolate reductase (ECs0051), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA26), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (CI641_024860), Dihydrofolate reductase (dfr2d), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase type A13 (dfrA13), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase type 2, Dihydrofolate reductase (C9108_15520), Dihydrofolate reductase (CSB64_27845), Dihydrofolate reductase (C9175_03430), Dihydrofolate reductase (dfr2a), Dihydrofolate reductase type 7 (dhfrVII), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA_2), Dihydrofolate reductase (dfr22), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfr22), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei68Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi32 – 36NADPUniRule annotation5

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductaseUniRule annotation
Biological processOne-carbon metabolismUniRule annotation
LigandNADPUniRule annotation

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00077;UER00158

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Dihydrofolate reductaseUniRule annotation (EC:1.5.1.3UniRule annotation)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:dfr2dImported
Synonyms:dfr2Imported, dfrB4Imported
ORF Names:EGT48_01415Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid pQepImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coliImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri562 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000278385 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unassembled WGS sequence

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotetramer.

UniRule annotation

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Phylogenomic databases

KEGG Orthology (KO)

More...
KOi
K19645

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.30.30.60, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR009159 Dhfr_type_II
IPR008990 Elect_transpt_acc-like_dom_sf
IPR023408 MscS_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF06442 DHFR_2, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000199 Dhfr_type_II, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50090 SSF50090, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8VNN1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNEGKNEVST SAAGRFAFPS NATFALGDRV RKKSGAAWQG RIVGWYCTTL
60 70
TPEGYAVESE SHPGSVQIYP MTALERVA
Length:78
Mass (Da):8,410
Last modified:March 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iAE14E2131AB6BB4E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY973253 Genomic DNA Translation: AAX84553.1
EU847537 Genomic DNA Translation: ACF70726.1
HQ902143 Genomic DNA Translation: ADZ24263.1
JF412519 Genomic DNA Translation: AEX58653.1
KF914308 Genomic DNA Translation: AIV43497.1
KP314737 Genomic DNA Translation: AKF12264.1
KP314738 Genomic DNA Translation: AKF12266.1
KP789952 Genomic DNA Translation: AKU38043.1
AJ429132 Genomic DNA Translation: CAD22098.1
RKJN01000002 Genomic DNA Translation: RSB75670.1

NCBI Reference Sequences

More...
RefSeqi
WP_024167031.1, NZ_SCJW01000116.1

Genome annotation databases

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ag:CAD22098

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY973253 Genomic DNA Translation: AAX84553.1
EU847537 Genomic DNA Translation: ACF70726.1
HQ902143 Genomic DNA Translation: ADZ24263.1
JF412519 Genomic DNA Translation: AEX58653.1
KF914308 Genomic DNA Translation: AIV43497.1
KP314737 Genomic DNA Translation: AKF12264.1
KP314738 Genomic DNA Translation: AKF12266.1
KP789952 Genomic DNA Translation: AKU38043.1
AJ429132 Genomic DNA Translation: CAD22098.1
RKJN01000002 Genomic DNA Translation: RSB75670.1
RefSeqiWP_024167031.1, NZ_SCJW01000116.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Genome annotation databases

KEGGiag:CAD22098

Phylogenomic databases

KOiK19645

Enzyme and pathway databases

UniPathwayiUPA00077;UER00158

Family and domain databases

Gene3Di2.30.30.60, 1 hit
InterProiView protein in InterPro
IPR009159 Dhfr_type_II
IPR008990 Elect_transpt_acc-like_dom_sf
IPR023408 MscS_dom_sf
PfamiView protein in Pfam
PF06442 DHFR_2, 1 hit
PIRSFiPIRSF000199 Dhfr_type_II, 1 hit
SUPFAMiSSF50090 SSF50090, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiQ8VNN1_ECOLX
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8VNN1
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2002
Last sequence update: March 1, 2002
Last modified: July 31, 2019
This is version 74 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, PlasmidImported
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