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UniProtKB - Q9JIA7 (SPHK2_MOUSE)

Entry version 161 (16 Oct 2019)
Sequence version 2 (27 Mar 2002)
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Protein

Sphingosine kinase 2

Gene

Sphk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides (PubMed:16118219, PubMed:10751414). In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis (PubMed:16118219). In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration. The SPP produced in mitochondria binds PHB2 and modulates the regulation via PHB2 of complex IV assembly and respiration (PubMed:20959514). In nucleus, plays a role in epigenetic regulation of gene expression. Interacts with HDAC1 and HDAC2 and, through SPP production, inhibits their enzymatic activity, preventing the removal of acetyl groups from lysine residues with histones. Upregulates acetylation of histone H3-K9, histone H4-K5 and histone H2B-K12. In nucleus, may have an inhibitory effect on DNA synthesis and cell cycle (By similarity). In mast cells, is the main regulator of SPP production which mediates calcium influx, NF-kappa-B activation, cytokine production, such as TNF and IL6, and degranulation of mast cells (PubMed:17346996). In dopaminergic neurons, is involved in promoting mitochondrial functions regulating ATP and ROS levels (PubMed:25637806). Also involved in the regulation of glucose and lipid metabolism (PubMed:30593892).By similarity6 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=3.4 µM for sphing-4-enine1 Publication
  1. Vmax=0.1 nmol/min/mg enzyme1 Publication
  2. Vmax=0.3 nmol/min/mg enzyme (in the presence of 50 mM KCl)
  3. Vmax=1 nmol/min/mg enzyme (in the presence of 200 mM KCl)

pH dependencei

Optimum pH is 7.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei212Proton donor/acceptorBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei217ATPPROSITE-ProRule annotation1
Binding sitei309SubstrateBy similarity1
Binding sitei316ATPPROSITE-ProRule annotation1
Binding sitei322ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi153 – 155ATPPROSITE-ProRule annotation3
Nucleotide bindingi185 – 189ATPPROSITE-ProRule annotation5
Nucleotide bindingi242 – 244ATPPROSITE-ProRule annotation3
Nucleotide bindingi586 – 588ATPPROSITE-ProRule annotation3

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.1.91 3474

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-1660661 Sphingolipid de novo biosynthesis

SABIO-RK: Biochemical Reaction Kinetics Database

More...SABIO-RKi		Q9JIA7

Chemistry databases

SwissLipids knowledge resource for lipid biology

More...SwissLipidsi		SLP:000000113

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Sphingosine kinase 2Curated (EC:2.7.1.913 Publications)
Short name:
SK 2
Short name:
SPK 2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Sphk2Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 7

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:1861380 Sphk2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Lysosome, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Mutant mice are viable, fertile and have normal longevity. They show reduced SPP levels (PubMed:16314531). Mice have decreased fat mass but increased lean mass, they display increased energy expenditure compared to wild-type. Aging mice are protected from metabolic decline and obesity. 52-week old male mutant mice have decreased weight and fat mass, and increased glucose tolerance and insulin sensitivity compared to control mice (PubMed:30593892). Double knockout for SPHK1 and SPHK2 causes embryonic lethality (PubMed:16314531). Between 11.5 dpc and 12.5 dpc embryos exhibit cranial hemorrhage and die at 13.5 dpc (PubMed:16314531). At 11.5 dpc the wall of the dorsal aorta is poorly developed and endothelial cells are severely defective in all blood vessels in the mesenchymal region of the head (PubMed:16314531). Embryos also show a neural tube defect (PubMed:16314531).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi93 – 94RR → EE: Loss of nuclear location. Loss of DNA synthesis inhibition. 1 Publication2
Mutagenesisi213G → E: Abolishes sphingosine kinase activity. Decreases apoptosis induction. Abolishes sphingosine kinase activity. No effect on endoplasmic reticulum location increase upon serum starvation. Abolishes sphingosine kinase activity and decreases apoptosis induction; when associated with A-219. 1 Publication1
Mutagenesisi219L → A: Strongly decreases sphingosine kinase activity. Decreases apoptosis induction. No effect on endoplasmic reticulum location increase upon serum starvation. Abolishes sphingosine kinase activity and decreases apoptosis induction; when associated with E-213. 1 Publication1

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL1075305

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001813591 – 617Sphingosine kinase 2Add BLAST617

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei358PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei377PhosphothreonineCombined sources1
Modified residuei384PhosphoserineBy similarity1
Modified residuei386PhosphoserineBy similarity1
Modified residuei578PhosphothreonineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by PKD on Ser-384 and Ser-386 upon PMA treatment. Phosphorylation induces export from the nucleus to the cytoplasm. Phosphorylated by MAPK1 and MAPK2 at Thr-578, phosphorylation is induced by agonists such as EGF and PMA and increases kinase activity.By similarity
Cleaved by CASP1 in apoptotic cells. The truncated form is released from cells.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q9JIA7

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q9JIA7

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q9JIA7

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q9JIA7

PRoteomics IDEntifications database

More...PRIDEi		Q9JIA7

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q9JIA7

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q9JIA7

SwissPalm database of S-palmitoylation events

More...SwissPalmi		Q9JIA7

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in heart, brain, liver, kidney and testis (PubMed:10751414). Expressed by mast cells (at protein level) (PubMed:17346996). In the substantia nigra, expressed by dopaminergic neurons (at protein level) (PubMed:25637806).3 Publications

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

At 10.5 dpc, expression is relatively ubiquitous with the strongest signals detected in the limb buds, eyes and branchial arches and a weaker expression in the telencephalon and spinal cord.1 Publication

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expressopm decreases upon treatment with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) which is used to induce Parkinson disease in mouse model.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000057342 Expressed in 256 organ(s), highest expression level in ear vesicle

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q9JIA7 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q9JIA7 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with histone H3.

Interacts with HDAC1, HDAC2, MBD2 and SIN3A.

Interacts with EEF1A1; the interaction enhances SPHK2 kinase activity (By similarity).

Interacts with PHB2 (PubMed:20959514).

By similarity1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
P396882EBI-985434,EBI-524514

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

Protein interaction database and analysis system

More...IntActi		Q9JIA7, 6 interactors

Molecular INTeraction database

More...MINTi		Q9JIA7

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000072615

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		Q9JIA7

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q9JIA7

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini143 – 290DAGKcPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 140Required for binding to sulfatide and phosphoinositides and for membrane localizationBy similarityAdd BLAST140
Regioni210 – 213Substrate bindingBy similarity4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi87 – 95NLS1 Publication9
Motifi381 – 390NESBy similarity10

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1116 Eukaryota
COG1597 LUCA

Ensembl GeneTree

More...GeneTreei		ENSGT00940000161197

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		HOG000111460

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q9JIA7

KEGG Orthology (KO)

More...KOi		K04718

Identification of Orthologs from Complete Genome Data

More...OMAi		YVSHVTC

Database of Orthologous Groups

More...OrthoDBi		681139at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q9JIA7

TreeFam database of animal gene trees

More...TreeFami		TF354296

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.50.10330, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00781 DAGK_cat, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00046 DAGKc, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF111331 SSF111331, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50146 DAGK, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9JIA7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPPPLLPVA ASTPILHGEF GSYPANGPRF ALTLTTQALH IQRLRPKPEA 
        60         70         80         90        100
RPRDGLVSLD EVSGCGTLQS RSPEDTAAYF CIYTYPRGRR GGRRRATRTF 
       110        120        130        140        150
RADGATTYEE NRAEAQRWAT ALTCLLRGVP LSGDQEITPE LLPRKPRLLI 
       160        170        180        190        200
LVNPFGGRGL AWQRCMDHVV PMISEAGLSF NLIQTERQNH ARELVQGLSL 
       210        220        230        240        250
SEWEGIVTVS GDGLLYEVLN GLLDRPDWED AVRMPIGVLP CGSGNALAGA 
       260        270        280        290        300
VNHHGGFEQV VGVDLLLNCS LLLCRGGSHP LDLLSVTLAS GSRCFSFLSV 
       310        320        330        340        350
AWGFLSDVDI HSERFRALGS ARFTLGAVLG LASLHTYRGR LSYLPATTEP 
       360        370        380        390        400
ALPIPGHSLP RAKSELVLAP APAPAATHSP LHRSVSDLPL PLPQPALVSP 
       410        420        430        440        450
GSPEPLPDLS LNGGGPELTG DWGGAGDAPL SPDPLLPSSP NALKTAQLSP 
       460        470        480        490        500
IAEGPPEMPA SSGFLPPTHS APEASTWGPV DHLLPPLGSP LPQDWVTIEG 
       510        520        530        540        550
EFVLMLGILP SHLCADLMAA PHARFDDGVV HLCWVRSGIS RAALLRILLA 
       560        570        580        590        600
MEHGNHFSLG CPHLGYAAAR AFRLEPLTPR GLLTVDGELV EYGPIQAQVH 
       610 
PGLATLLTGP AGQKPQA
Length:617
Mass (Da):65,618
Last modified:March 27, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i40EE2C2C288BE26A
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GS43A0A1B0GS43_MOUSE
Sphingosine kinase 2
Sphk2
131Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti252N → S in AAF74125 (PubMed:10751414).Curated1
Sequence conflicti510P → T in AAF74125 (PubMed:10751414).Curated1
Sequence conflicti548L → F in AAF74125 (PubMed:10751414).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF245448 mRNA Translation: AAF74125.1
AF415214 mRNA Translation: AAL07500.1
AK004951 mRNA Translation: BAB23694.1
BC006941 mRNA Translation: AAH06941.1
BC053737 mRNA Translation: AAH53737.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS21261.1

NCBI Reference Sequences

More...RefSeqi		NP_001166032.1, NM_001172561.1
NP_064395.2, NM_020011.5
NP_975009.1, NM_203280.3
XP_006541075.1, XM_006541012.3
XP_006541076.1, XM_006541013.3

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000072836; ENSMUSP00000072615; ENSMUSG00000057342
ENSMUST00000107737; ENSMUSP00000103366; ENSMUSG00000057342
ENSMUST00000210060; ENSMUSP00000147391; ENSMUSG00000057342

Database of genes from NCBI RefSeq genomes

More...GeneIDi		56632

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:56632

UCSC genome browser

More...UCSCi		uc009gwt.2 mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245448 mRNA Translation: AAF74125.1
AF415214 mRNA Translation: AAL07500.1
AK004951 mRNA Translation: BAB23694.1
BC006941 mRNA Translation: AAH06941.1
BC053737 mRNA Translation: AAH53737.1
CCDSiCCDS21261.1
RefSeqiNP_001166032.1, NM_001172561.1
NP_064395.2, NM_020011.5
NP_975009.1, NM_203280.3
XP_006541075.1, XM_006541012.3
XP_006541076.1, XM_006541013.3

3D structure databases

SMRiQ9JIA7
ModBaseiSearch...

Protein-protein interaction databases

IntActiQ9JIA7, 6 interactors
MINTiQ9JIA7
STRINGi10090.ENSMUSP00000072615

Chemistry databases

BindingDBiQ9JIA7
ChEMBLiCHEMBL1075305
SwissLipidsiSLP:000000113

PTM databases

iPTMnetiQ9JIA7
PhosphoSitePlusiQ9JIA7
SwissPalmiQ9JIA7

Proteomic databases

EPDiQ9JIA7
jPOSTiQ9JIA7
MaxQBiQ9JIA7
PaxDbiQ9JIA7
PRIDEiQ9JIA7

Protocols and materials databases

The DNASU plasmid repository

More...DNASUi		56632

Genome annotation databases

EnsembliENSMUST00000072836; ENSMUSP00000072615; ENSMUSG00000057342
ENSMUST00000107737; ENSMUSP00000103366; ENSMUSG00000057342
ENSMUST00000210060; ENSMUSP00000147391; ENSMUSG00000057342
GeneIDi56632
KEGGimmu:56632
UCSCiuc009gwt.2 mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		56848
MGIiMGI:1861380 Sphk2

Phylogenomic databases

eggNOGiKOG1116 Eukaryota
COG1597 LUCA
GeneTreeiENSGT00940000161197
HOGENOMiHOG000111460
InParanoidiQ9JIA7
KOiK04718
OMAiYVSHVTC
OrthoDBi681139at2759
PhylomeDBiQ9JIA7
TreeFamiTF354296

Enzyme and pathway databases

BRENDAi2.7.1.91 3474
ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis
SABIO-RKiQ9JIA7

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Sphk2 mouse

Protein Ontology

More...PROi		PR:Q9JIA7

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000057342 Expressed in 256 organ(s), highest expression level in ear vesicle
ExpressionAtlasiQ9JIA7 baseline and differential
GenevisibleiQ9JIA7 MM

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPHK2_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9JIA7
Secondary accession number(s): Q91VA9, Q9DBH6
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: March 27, 2002
Last modified: October 16, 2019
This is version 161 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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