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Entry version 150 (08 May 2019)
Sequence version 1 (01 Nov 1999)
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Protein

Mitotic checkpoint protein bub-3

Gene

bub-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments (PubMed:18248670, PubMed:19109417, PubMed:25987605). Promotes the formation of stable end-on bipolar attachments of chromosomes (PubMed:25987605). Necessary for expression and kinetochore localization of bub-1 (PubMed:19109417, PubMed:25987605). Plays a role in synapsis checkpoint signaling inducing apoptosis in response to unsynapsed chromosomes and thus controlling chromosomal segregation during oocyte meiosis (PubMed:26483555).4 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • ubiquitin binding Source: GO_Central

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-CEL-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-CEL-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-CEL-179409 APC-Cdc20 mediated degradation of Nek2A

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...
SignaLinki
Q9XWH0

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mitotic checkpoint protein bub-3By similarity
Alternative name(s):
Budding uninhibited by benzimidazole 3By similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:bub-3Imported
ORF Names:Y54G9A.6Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCaenorhabditis elegansImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri6239 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001940 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome II

Organism-specific databases

WormBase

More...
WormBasei
Y54G9A.6 ; CE19233 ; WBGene00013209 ; bub-3

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Viable, but with a reduced brood size (PubMed:25987605). Reduced bub-1 expression and localization at the kinetochore which may lead to the delay in the onset of anaphase during embryogenesis (PubMed:25987605). Defective synapsis with increased numbers of nuclei that contain asymmetrically aligned (clustered) chromosomes (PubMed:26483555). RNAi-mediated knockdown results in reduced survival as compared to wild-type animals in anoxic conditions (PubMed:18248670).3 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004366071 – 343Mitotic checkpoint protein bub-3CuratedAdd BLAST343

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q9XWH0

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9XWH0

PeptideAtlas

More...
PeptideAtlasi
Q9XWH0

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
WBGene00013209 Expressed in 5 organ(s), highest expression level in germ line (C elegans)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

May interact with bub-1; for localization at the kinetochore and the onset of anaphase.

2 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
san-1O020535EBI-331258,EBI-316339

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-400 Bub-1-Bub-3 complex
CPX-401 Mitotic checkpoint complex, Mad-1-Mad-2-Bub-1-Bub-3 subcomplex

Database of interacting proteins

More...
DIPi
DIP-26143N

Protein interaction database and analysis system

More...
IntActi
Q9XWH0, 7 interactors

STRING: functional protein association networks

More...
STRINGi
6239.Y54G9A.6

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati21 – 62WD 1Sequence analysisAdd BLAST42
Repeati67 – 105WD 2Sequence analysisAdd BLAST39
Repeati107 – 146WD 3Sequence analysisAdd BLAST40
Repeati150 – 187WD 4Sequence analysisAdd BLAST38
Repeati192 – 232WD 5Sequence analysisAdd BLAST41
Repeati249 – 288WD 6Sequence analysisAdd BLAST40
Repeati291 – 331WD 7Sequence analysisAdd BLAST41

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the WD repeat BUB3 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1036 Eukaryota
ENOG410XRBG LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00950000183091

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000208822

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q9XWH0

KEGG Orthology (KO)

More...
KOi
K02180

Identification of Orthologs from Complete Genome Data

More...
OMAi
SWDSTLH

Database of Orthologous Groups

More...
OrthoDBi
1048963at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9XWH0

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.130.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00400 WD40, 2 hits

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00320 GPROTEINBRPT

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00320 WD40, 5 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50978 SSF50978, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9XWH0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTYQAATIV AAPNEFRVPF PPFVQISKVQ FQREAGSRLL AASGWDGTCR
60 70 80 90 100
VYEVGKLGDI SEKLVFTHGK PLLTCTFAGY NKVAFGGVDH NVKLADIETG
110 120 130 140 150
NGTQLGSHAL AVRCMEFNPM SSLIVSGGWD SSVKLWDARS YGNGAIESVN
160 170 180 190 200
VSSSVYAMDV LKHTILVGTK DRKIFMYDSR KLREPLQVRD SPLKYQTRAV
210 220 230 240 250
QFFPTGEAFV VSSIEGRVAV EYVDQSGEEM MKRKYAFKCH REKDTDGTEL
260 270 280 290 300
IHPVHTVAFH PKYGTFATGG ADGIVNIWDP FNRKRIIQLH KFETSISSLS
310 320 330 340
FNEDGSQLAI ATSYQYEKEI DPSPLPNNSI TIRHITDPES RPK
Length:343
Mass (Da):38,127
Last modified:November 1, 1999 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i021AD0398C6BD5ED
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
BX284602 Genomic DNA Translation: CAA21698.1

Protein sequence database of the Protein Information Resource

More...
PIRi
T27185

NCBI Reference Sequences

More...
RefSeqi
NP_496879.1, NM_064478.4

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
Y54G9A.6; Y54G9A.6; WBGene00013209

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
175018

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
cel:CELE_Y54G9A.6

UCSC genome browser

More...
UCSCi
Y54G9A.6 c. elegans

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284602 Genomic DNA Translation: CAA21698.1
PIRiT27185
RefSeqiNP_496879.1, NM_064478.4

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

ComplexPortaliCPX-400 Bub-1-Bub-3 complex
CPX-401 Mitotic checkpoint complex, Mad-1-Mad-2-Bub-1-Bub-3 subcomplex
DIPiDIP-26143N
IntActiQ9XWH0, 7 interactors
STRINGi6239.Y54G9A.6

Proteomic databases

EPDiQ9XWH0
PaxDbiQ9XWH0
PeptideAtlasiQ9XWH0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY54G9A.6; Y54G9A.6; WBGene00013209
GeneIDi175018
KEGGicel:CELE_Y54G9A.6
UCSCiY54G9A.6 c. elegans

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
175018
WormBaseiY54G9A.6 ; CE19233 ; WBGene00013209 ; bub-3

Phylogenomic databases

eggNOGiKOG1036 Eukaryota
ENOG410XRBG LUCA
GeneTreeiENSGT00950000183091
HOGENOMiHOG000208822
InParanoidiQ9XWH0
KOiK02180
OMAiSWDSTLH
OrthoDBi1048963at2759
PhylomeDBiQ9XWH0

Enzyme and pathway databases

ReactomeiR-CEL-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-CEL-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-CEL-179409 APC-Cdc20 mediated degradation of Nek2A
SignaLinkiQ9XWH0

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q9XWH0

Gene expression databases

BgeeiWBGene00013209 Expressed in 5 organ(s), highest expression level in germ line (C elegans)

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 2 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBUB3_CAEEL
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9XWH0
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 6, 2016
Last sequence update: November 1, 1999
Last modified: May 8, 2019
This is version 150 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
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