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Entry version 23 (23 May 2018)
Sequence version 1 (18 Sep 2013)
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Protein

Type IV pilus biogenesis factor PilY1

Gene

pilY1

Organism
Pseudomonas aeruginosa (strain PAK)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in pilus assembly, twitching motility and adhesion to host cells. Primes type IV pili (T4P) assembly and is required for inclusion of minor pilins PilV, PilW and PilX to the surface pili (By similarity). Stabilizes assembled pilus fibers likely by antagonizing retraction mediated by PilT. Calcium-binding and calcium release by PilY1 seem to be essential for twitching motility and for regulation of pilus retraction dynamics of PilT. Adhesin for human tissue specifically recognizing a host receptor localized or enriched on basolateral epithelial cell surfaces. Binds host integrins in an calcium-dependent manner in vitro and this interaction may be employed by the bacterium to mediate host epithelial cell binding in vivo.By similarity3 Publications

Miscellaneous

Residues 600-608 comprise a calcium-binding site which together with the other one (residues 851-859) seems important for interaction with integrins of the host.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi851Calcium1 Publication1
Metal bindingi853Calcium1 Publication1
Metal bindingi855Calcium1 Publication1
Metal bindingi857Calcium; via carbonyl oxygen1 Publication1
Metal bindingi859Calcium1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processFimbrium biogenesis
LigandCalcium, Metal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Type IV pilus biogenesis factor PilY11 Publication
Alternative name(s):
Pilus-associated adhesin PilY1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pilY1Imported
ORF Names:PAK_05020Imported, Y880_0129235Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPseudomonas aeruginosa (strain PAK)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1009714 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Fimbrium, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Loss of surface T4P which is not due to the lack of major pilus structural subunit PilA (PubMed:20080557, PubMed:8899718, PubMed:20331639). Lacks measurable twitching motility (PubMed:20080557, PubMed:8899718). T4P fibers are restored when pilus retraction ATPase pilT is deleted at the same time. Does not bind to the injured regions of human ciliated airway epithelium (HAE) during bacterial infection on the contrary to the wild-type which preferentially binds to the injured regions of the HAE (PubMed:20331639).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi859D → A: Loss of calcium-binding. Significantly reduced twitching motility. Dramatically reduced level of surface T4P. Regains ability to produce normal levels of T4P and is capable of trafficking to the T4P fraction; when associated with pilT deletion mutant. 1 Publication1
Mutagenesisi859D → K: Loss of calcium-binding. Significantly reduced twitching motility. Insensitive to the effects of calcium chelator EGTA and produces an abundance of non-functional surface T4P. Capable of trafficking to the T4P fraction. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 30ImportedAdd BLAST30
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000043191731 – 1163Type IV pilus biogenesis factor PilY1ImportedAdd BLAST1133

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts (via C-terminal 532-1163) with host integrins alpha-V/beta-3 (ITGAV/ITGB3) and alpha-V/beta-5 (ITGAV/ITGB5).

1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
S0HPF7

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni600 – 608Calcium-binding1 Publication9
Regioni619 – 621Integrin-binding motif RGD1 Publication3

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the PilY1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008707 PilC_beta_prop_dom
IPR011047 Quinoprotein_ADH-like_supfam

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF05567 Neisseria_PilC, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50998 SSF50998, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

S0HPF7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSALHQIGK TSLAAALSGA VLLSAQTTHA AALSVSQQPL MLIQGVAPNM
60 70 80 90 100
LVTLDDSGSM AYAYAPDSLV NSRNNVYFAS NSYNPMYFDP NTQYKLPKKV
110 120 130 140 150
TLSNGQIQVQ DYSKPSFTAA WRNGFTQEGR VNLSRDYRPT VQYQGGSGAG
160 170 180 190 200
TESSIDWYGA PAFYYQYSGG RGCSLTTSSC YTRVEISGAA QQQNFANWYS
210 220 230 240 250
FYRTRALATQ TAANLAFYSL PENARISWQL LNSSSCLIGS GSSNCYNNYL
260 270 280 290 300
RDFTGQHRVN FFNWLENLSV GGGTPLRQAM TRAGEFLKKT GVNGPYAYRP
310 320 330 340 350
GTQTSPEYSC RGSYHILMTD GLWNNDSASV GNADSTSRSL PDGKSYSSQT
360 370 380 390 400
PYRDAASNTL ADQAFHYWAT DARPDIDDNI KPYIPYPDQA NPSAEYWNPR
410 420 430 440 450
NDPATWQHMV TYTLGLGLTT SLTSPKWEGS TYSGGYDEIA AGRLSWPNAS
460 470 480 490 500
NNHSNNVYDL WHAAVNSRGE FFSADSPDQL VAAFQDILNR ISGKDLPASR
510 520 530 540 550
PAISSSLQED DTGDKLTRFA YQTSFASDKN WAGDLTRYSL TTQDKATVQT
560 570 580 590 600
KLWSAQSILD AMPNGGAGRK IMMAGSGTSG LKEFTWGSLS ADQQRQLNRD
610 620 630 640 650
PDRNDVADTK GQDRVAFLRG DRSKENSDNF RTRNSILGDI INSSPATVGK
660 670 680 690 700
AQYLTYLAQP IEPSGNYSTF AEAQKTRAPR VYVGANDGML HGFDTDGNET
710 720 730 740 750
FAFIPSAVFE KLHKLTARGY QGGAHQFYVD GSPVVADAFF GGAWHTVLIG
760 770 780 790 800
SLRAGGKGLF ALDVTDPANI KLLWEIGVDQ EPDLGYSFPK PTVARLHNGK
810 820 830 840 850
WAVVTGNGYS SLNDKAALLI IDLETGAITR KLEVTGRTGV PNGLSSPRLA
860 870 880 890 900
DNNSDGVADY AYAGDLQGNL WRFDLIAGKV NQDDPFSRAN DGPAVASSFR
910 920 930 940 950
VSFGGQPLYS AVDSAGAAQA ITAAPSLVRH PTRKGYIVIF GTGKYFENAD
960 970 980 990 1000
ARADTSRAQT LYGIWDQQTK GEAAGSTPRL TRGNLQQQTL DLQADSTFAS
1010 1020 1030 1040 1050
TARTIRIASQ NPVNWLNNDG STKQSGWYLD FMVNGTLKGE MLIEDMIAIG
1060 1070 1080 1090 1100
QVVLLQTITP NDDPCADGAS NWTYGLDPYT GGRTSFTVFD LARQGVVDSK
1110 1120 1130 1140 1150
SDYSYNKQNV AVSGTEQKGL GGLTLSTNEQ GNPEVCSSGE CLTVNPGPNT
1160
RGRQNWRPIE GKN
Length:1,163
Mass (Da):126,248
Last modified:September 18, 2013 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7FCED3BE18D5FC52
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
EU234515 Genomic DNA Translation: ABW89597.1
ASWU01000022 Genomic DNA Translation: EOT10199.1
AZUG01000415 Genomic DNA Translation: KAJ87266.1

NCBI Reference Sequences

More...
RefSeqi
WP_010793785.1, NZ_KE137323.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
EOT10199; EOT10199; PAK_05020
KAJ87266; KAJ87266; Y880_0129235

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1009714.6.peg.5062

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU234515 Genomic DNA Translation: ABW89597.1
ASWU01000022 Genomic DNA Translation: EOT10199.1
AZUG01000415 Genomic DNA Translation: KAJ87266.1
RefSeqiWP_010793785.1, NZ_KE137323.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HX6X-ray2.10A/B615-1163[»]
SMRiS0HPF7
ModBaseiSearch...
PDBe-KBiSearch...

Genome annotation databases

EnsemblBacteriaiEOT10199; EOT10199; PAK_05020
KAJ87266; KAJ87266; Y880_0129235
PATRICifig|1009714.6.peg.5062

Family and domain databases

InterProiView protein in InterPro
IPR008707 PilC_beta_prop_dom
IPR011047 Quinoprotein_ADH-like_supfam
PfamiView protein in Pfam
PF05567 Neisseria_PilC, 1 hit
SUPFAMiSSF50998 SSF50998, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPILY1_PSEAW
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: S0HPF7
Secondary accession number(s): A8WE64
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: September 18, 2013
Last modified: May 23, 2018
This is version 23 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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