| http://purl.uniprot.org/citations/10022888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10022888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/10022888 | http://www.w3.org/2000/01/rdf-schema#comment | "The assembly pathway of spliceosomal snRNPs in yeast is poorly understood. We devised a screen to identify mutations blocking the assembly of newly synthesized U4 snRNA into a functional snRNP. Fifteen mutant strains failing either to accumulate the newly synthesized U4 snRNA or to assemble a U4/U6 particle were identified and categorized into 13 complementation groups. Thirteen previously identified splicing-defective prp mutants were also assayed for U4 snRNP assembly defects. Mutations in the U4/U6 snRNP components Prp3p, Prp4p, and Prp24p led to disassembly of the U4/U6 snRNP particle and degradation of the U6 snRNA, while prp17-1 and prp19-1 strains accumulated free U4 and U6 snRNA. A detailed analysis of a newly identified mutant, the sad1-1 mutant, is presented. In addition to having the snRNP assembly defect, the sad1-1 mutant is severely impaired in splicing at the restrictive temperature: the RP29 pre-mRNA strongly accumulates and splicing-dependent production of beta-galactosidase from reporter constructs is abolished, while extracts prepared from sad1-1 strains fail to splice pre-mRNA substrates in vitro. The sad1-1 mutant is the only splicing-defective mutant analyzed whose mutation preferentially affects assembly of newly synthesized U4 snRNA into the U4/U6 particle. SAD1 encodes a novel protein of 52 kDa which is essential for cell viability. Sad1p localizes to the nucleus and is not stably associated with any of the U snRNAs. Sad1p contains a putative zinc finger and is phylogenetically highly conserved, with homologues identified in human, Caenorhabditis elegans, Arabidospis, and Drosophila."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.19.3.2008"xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.19.3.2008"xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/author | "Seraphin B."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/author | "Seraphin B."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/author | "Lygerou Z."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/author | "Lygerou Z."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/author | "Christophides G."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/author | "Christophides G."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/pages | "2008-2020"xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/pages | "2008-2020"xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/title | "A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/title | "A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing."xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://purl.uniprot.org/core/volume | "19"xsd:string |
| http://purl.uniprot.org/citations/10022888 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10022888 |
| http://purl.uniprot.org/citations/10022888 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10022888 |
| http://purl.uniprot.org/citations/10022888 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10022888 |
| http://purl.uniprot.org/citations/10022888 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/10022888 |