http://purl.uniprot.org/citations/10029623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/10029623 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/10029623 | http://www.w3.org/2000/01/rdf-schema#comment | "Background & aimsAnti-liver cytosol type 1 autoantibodies have been reported in association with anti-liver-kidney microsome type 1 autoantibodies in 30% of patients with autoimmune hepatitis type II. In 10% of cases, anti-liver cytosol type 1 antibodies are the only liver-related circulating autoantibodies. The liver cytosol antigen is a liver-specific 62-kilodalton protein present in the cell as an oligomer of approximately 240 kilodaltons. The aim of this study was to identify the antigen recognized by anti-liver cytosol antibody.MethodsTo identify the liver cytosol antigen, an anti-liver cytosol type 1-positive serum was used for the screening of a complementary DNA library from HepG2 cells. Double immunodiffusion method was used to show the identity between the cytosolic and the cloned protein.ResultsThe sequence of two isolated clones showed 85.2% homology with the formiminotransferase cyclodeaminase (FTCD) enzyme from pig liver. Antibodies purified by affinity with the recombinant protein and sera from mice immunized with FTCD recognized a 62-kilodalton human cytosolic protein when tested by immunoblot. The identity of precipitation lines was found between the cytosolic antigen and FTCD.ConclusionsThis enzyme is a liver-specific antigen recognized by the sera of patients with autoimmune hepatitis."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0016-5085(99)70186-1"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0016-5085(99)70186-1"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Lapierre P."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Lapierre P."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Alvarez F."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Alvarez F."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Hajoui O."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Hajoui O."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Homberg J.-C."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/author | "Homberg J.-C."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/name | "Gastroenterology"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/name | "Gastroenterology"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/pages | "643-649"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/pages | "643-649"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/title | "Formiminotransferase cyclodeaminase is an organ-specific autoantigen recognized by sera of patients with autoimmune hepatitis."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/title | "Formiminotransferase cyclodeaminase is an organ-specific autoantigen recognized by sera of patients with autoimmune hepatitis."xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/volume | "116"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://purl.uniprot.org/core/volume | "116"xsd:string |
http://purl.uniprot.org/citations/10029623 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10029623 |
http://purl.uniprot.org/citations/10029623 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/10029623 |